LOCUS Exported 5589 bp ds-DNA circular SYN 14-PAŹ-2016 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS pKK-TEV-ProteinA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5589) AUTHORS . TITLE Direct Submission JOURNAL Exported 14 paza 2016 from SnapGene 3.0.3 http://www.snapgene.com COMMENT LSOWNER| FEATURES Location/Qualifiers source 1..5589 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 232..958 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" protein_bind 820..838 /bound_moiety="repressor" /note="tet operator" protein_bind 841..859 /bound_moiety="repressor" /note="tet operator" misc_feature 1002..1022 /note="TEV-L" /note="enocdes peptide cleaved by TEV protease" misc_feature 1063..1083 /note="TEV-R" /note="enocdes peptide cleaved by TEV protease" CDS 1090..1503 /codon_start=1 /note="protein A" /translation="KTAALAQHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSL KDDPSQSANLLAEAKKLNDAQAPKVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK DDPSQSANLLAEAKKLNGAQAPKVDANSAGKST" polyA_signal 1547..1771 /note="BGH poly(A)signal" /note="bovine growth hormone polyadenylation signal" protein_bind 2055..2102 /bound_moiety="FLP recombinase" /note="FRT" CDS 2110..3132 /codon_start=1 /product="aminoglycoside phosphotransferase from E. coli" /note="Hygromycin B" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE " polyA_signal 3262..3392 /note="SV40 poly(A)signal" /note="SV40 polyadenylation signal" rep_origin complement(3775..4448) /direction=LEFT /note="pUC ori" CDS complement(4593..5453) /codon_start=1 /product="beta-lactamase" /note="AmpR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature complement(5454..5552) /note="Amp promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 961 ctctggcgtt taaacttaag cttggtaccg agctcggatc cgaaaacctg tacttccaag 1021 gaaccggtgg tcacctgtac aatcgatcct gcaggagcta gcgagaattt gtattttcag 1081 ggtgatatca aaaccgcggc tcttgcgcaa cacgatgaag ccgtggacaa caaattcaac 1141 aaagaacaac aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa cgaagaacaa 1201 cgaaacgcct tcatccaaag tttaaaagat gacccaagcc aaagcgctaa ccttttagca 1261 gaagctaaaa agctaaatga tgctcaggcg ccgaaagtag acaacaaatt caacaaagaa 1321 caacaaaacg cgttctatga gatcttacat ttacctaact taaacgaaga acaacgaaac 1381 gccttcatcc aaagtttaaa agatgaccca agccaaagcg ctaacctttt agcagaagct 1441 aaaaagctaa atggtgctca ggcgccgaaa gtagacgcga attccgcggg gaagtcaacc 1501 tgactcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt 1561 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact 1621 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat 1681 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc 1741 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc 1801 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 1861 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 1921 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 1981 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 2041 ggttcacgta cctagaagtt cctattccga agttcctatt ctctagaaag tataggaact 2101 tccttggcca aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag 2161 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc 2221 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac 2281 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt 2341 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc 2401 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc 2461 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg 2521 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat 2581 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc 2641 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat 2701 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc 2761 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg 2821 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga 2881 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg 2941 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga 3001 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc 3061 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg 3121 gcaaaggaat agcacgtact acgagatttc gattccaccg ccgccttcta tgaaaggttg 3181 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 3241 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc 3301 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 3361 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg 3421 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 3481 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 3541 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 3601 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 3661 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 3721 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 3781 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 3841 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 3901 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 3961 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 4021 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 4081 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 4141 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 4201 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 4261 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 4321 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 4381 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 4441 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 4501 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 4561 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 4621 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 4681 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 4741 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 4801 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 4861 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 4921 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 4981 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 5041 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 5101 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 5161 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 5221 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 5281 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 5341 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 5401 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 5461 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 5521 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 5581 cctgacgtc //