LOCUS Exported 5880 bp ds-DNA circular SYN 28-MAY-2017 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS pKK-TEV-CyOFP1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5880) AUTHORS . TITLE Direct Submission JOURNAL Exported Sunday, May 28, 2017 from SnapGene 3.3.3 http://www.snapgene.com COMMENT LSOWNER| FEATURES Location/Qualifiers source 1..5880 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 232..958 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" protein_bind 820..838 /bound_moiety="repressor" /note="tet operator" protein_bind 841..859 /bound_moiety="repressor" /note="tet operator" misc_feature 1002..1022 /note="TEV-L" /note="enocdes peptide cleaved by TEV protease" misc_feature 1063..1083 /note="TEV-R" /note="enocdes peptide cleaved by TEV protease" CDS 1090..1794 /codon_start=1 /note="CyOFP1" /translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTN RIKVVEGGPLPFAFDILATHFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG VLTATQDTSLQDGELIYNVKVRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD KALKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY SNLGGGMDELYK" polyA_signal 1838..2062 /note="BGH poly(A)signal" /note="bovine growth hormone polyadenylation signal" protein_bind 2346..2393 /bound_moiety="FLP recombinase" /note="FRT" CDS 2401..3423 /codon_start=1 /product="aminoglycoside phosphotransferase from E. coli" /note="Hygromycin B" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE " polyA_signal 3553..3683 /note="SV40 poly(A)signal" /note="SV40 polyadenylation signal" rep_origin complement(4066..4739) /direction=LEFT /note="pUC ori" CDS complement(4884..5744) /codon_start=1 /product="beta-lactamase" /note="AmpR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature complement(5745..5843) /note="Amp promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 961 ctctggcgtt taaacttaag cttggtaccg agctcggatc cgaaaacctg tacttccaag 1021 gaaccggtgg tcacctgtac aatcgatcct gcaggagcta gcgagaattt gtattttcag 1081 ggtgatatca tggtgagcaa gggcgaggag ctgatcaagg agaacatgag aagcaagctg 1141 tacctggaag gcagcgtgaa cggccaccag ttcaagtgca cccacgaagg ggagggcaag 1201 ccctacgagg gcaagcagac caacaggatc aaggtggtgg agggaggccc cctgccgttc 1261 gcattcgaca tcctggccac ccactttatg tacgggagca aggtgttcat caagtacccc 1321 gccgacctcc ccgattattt taagcagtcc ttccctgagg gcttcacatg ggagagagtc 1381 atggtgttcg aagacggggg cgtgctgacc gccacccagg acaccagcct ccaggacggc 1441 gagctcatct acaacgtcaa ggtcagaggg gtgaacttcc cagccaacgg ccccgtgatg 1501 cagaagaaaa cactgggctg ggagcccagc accgagacca tgtaccccgc tgacggcggc 1561 ctggaaggca gatgcgacaa ggccctgaag ctcgtgggcg ggggccacct gcacgtcaac 1621 ttcaagacca catacaagtc caagaaaccc gtgaagatgc ccggcgtcca ctacgtggac 1681 cgcagactgg aaagaatcaa ggaggccgac aacgagacct acgtcgagca gtacgagcac 1741 gctgtggcca gatactccaa cctgggcgga ggcatggacg agctgtacaa gtaactcgag 1801 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca 1861 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 1921 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 1981 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 2041 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg 2101 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 2161 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 2221 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 2281 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 2341 acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttccttggcc 2401 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc 2461 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta 2521 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt 2581 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg 2641 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa 2701 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg 2761 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc 2821 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac 2881 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg 2941 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc 3001 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg 3061 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt 3121 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg 3181 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc 3241 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc 3301 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt 3361 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa 3421 tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga 3481 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc 3541 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 3601 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 3661 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca 3721 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 3781 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 3841 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 3901 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 3961 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 4021 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 4081 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 4141 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 4201 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 4261 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 4321 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 4381 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 4441 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 4501 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 4561 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 4621 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 4681 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 4741 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 4801 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 4861 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 4921 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 4981 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 5041 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 5101 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 5161 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 5221 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 5281 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 5341 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 5401 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 5461 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 5521 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 5581 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 5641 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 5701 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 5761 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 5821 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc //