LOCUS       pEMS1942        3695 bp    DNA     circular SYN  2-APR-2012
DEFINITION  Ple265 [cloned in pJ204].
ACCESSION   DNA2.0 Id: 77329
SOURCE      Synthetic.
  ORGANISM  Synthetic
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|617878608|
COMMENT     VNTDBDATE|622307545|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS1942|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "Ple265 [cloned in pJ204]." 1) 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/DNA2.0\">DNA2.0</a>; <a href=\"ncbilink:dnarna/Id:\">Id:</a>; <a href=\"ncbilink:dnarna/77329\">77329</a>" 
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FEATURES             Location/Qualifiers
     source          1..3695
                     /organism=synthetic
                     /vntifkey="98"
     gene            complement(1235..2234)
                     /gene=Ple265
                     /vntifkey="60"
     misc_feature    complement(1235..2234)
                     /product=Ple265
                     /vntifkey="21"
                     /label=Ple265
     misc_feature    complement(2851..3654)
                     /product=pUC_ori
                     /vntifkey="21"
                     /label=pUC_ori
     misc_feature    2566..2679
                     /product=rpn txn terminator
                     /vntifkey="21"
                     /label=rpn\txn\terminator
     misc_feature    2259..2559
                     /product=bla txn terminator
                     /vntifkey="21"
                     /label=bla\txn\terminator
     misc_feature    complement(1038..1212)
                     /product=rrnB1 B2 T1 txn terminator
                     /vntifkey="21"
                     /label=rrnB1\B2\T1\txn\terminator
     misc_feature    complement(2350..2375)
                     /product=pTF3
                     /vntifkey="21"
                     /label=pTF3
     misc_feature    1113..1129
                     /product=pTR
                     /vntifkey="21"
                     /label=pTR
     misc_feature    complement(8..856)
                     /product=Ampicilin
                     /vntifkey="21"
                     /label=Ampicilin
BASE COUNT      901 a      1006 c       874 g       914 t 
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 
     1201 cccgggctaa ttatggggtg tcgccctttt gacgggcgcg cctggactcc agtcactttt 
     1261 ctgctggcct ctgccccggc ccagtgccag agagggcctt ttaaacgtcc aggacgtggg 
     1321 ggtgtggatt ccccccatcc ccaaatcccc agctcatgcc ccatctgctc ccacccaagc 
     1381 ttaagcccca taaagatcat ccagataatt gtttgcccac aacgatgctg gatctggcat 
     1441 ggtgggtagg gggcagggcg acctgagctt ggacctcgct gtgcccagca gcaatgactg 
     1501 gaccccaggg actctcaggt gtgatgtcac aaaaggcctg agcagcgtcc ccacagttgg 
     1561 agaagggagg ccaacagtgt agggtggtag ctggcttagg gaggacttgc aggtgtcccc 
     1621 acccagccac ccaggttccc ccactggctc ctggaagggc agctgccacc tgcagtcggt 
     1681 aaagaaaaag gaggttggag tcaaaggccc cttccctccc tccctctctg cacctgcctc 
     1741 agacaccacc cgccacccta gctccagctc tgtctccttg gcagcaagtc aaggggtctc 
     1801 ccagccaccc ttccttcaac tcactgtccc tggagcctgg ttgcctgccc cagaccccaa 
     1861 aacaagaaag tctggggtct cctgttccca caccacctaa atgccaggtt gagctatctc 
     1921 ctctcctcta tcaccctagc cccaggtggg aacaggaggc gagactccct ggggacagag 
     1981 gcaggcgggg agaggatgtc cctgcctttt accccgagcc actgtggctt tccctcagtg 
     2041 cccaagcagg ggataccact gggaggtccc catccccttc ccttccctcc ccaagacgtc 
     2101 tttacggaaa ccagtggaag tttcatctca gaatgaatgg gctgcactat caccacctct 
     2161 gcccgagcct cctctgttcc acaaagggcc aagttcacac tctctccctc tatgccaatg 
     2221 aagtgtccac gcgtttcagc gaagggcgac acaaaattta ttctaaatgc ataataaata 
     2281 ctgataacat cttatagttt gtattatatt ttgtattatc gttgacatgt ataattttga 
     2341 tatcaaaaac tgattttccc tttattattt tcgagattta ttttcttaat tctctttaac 
     2401 aaactagaaa tattgtatat acaaaaaatc ataaataata gatgaatagt ttaattatag 
     2461 gtgttcatca atcgaaaaag caacgtatct tatttaaagt gcgttgcttt tttctcattt 
     2521 ataaggttaa ataattctca tatatcaagc aaagtgacag gcgcccttaa atattctgac 
     2581 aaatgctctt tccctaaact ccccccataa aaaaacccgc cgaagcgggt ttttacgtta 
     2641 tttgcggatt aacgattact cgttatcaga accgcccagg gggcccgagc ttaagactgg 
     2701 ccgtcgtttt acaacacaga aagagtttgt agaaacgcaa aaaggccatc cgtcaggggc 
     2761 cttctgctta gtttgatgcc tggcagttcc ctactctcgc cttccgcttc ctcgctcact 
     2821 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     2881 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     2941 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     3001 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     3061 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     3121 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 
     3181 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     3241 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     3301 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     3361 aggtatgtag gcggtgctac agagttcttg aagtggtggg ctaactacgg ctacactaga 
     3421 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     3481 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     3541 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     3601 gacgctcagt ggaacgacgc gcgcgtaact cacgttaagg gattttggtc atgagcttgc 
     3661 gccgtcccgt caagtcagcg taatgctctg ctttt 
//
