LOCUS       Exported                5818 bp ds-DNA     circular SYN 29-MAR-2018
DEFINITION  iCreERT2-FFrt-KanR-FFrt_110104 [cloned in pJ244].
ACCESSION   DNA2.0
VERSION     .
KEYWORDS    pEMS1725
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   UNKNOWN
  TITLE     Direct Submission
  JOURNAL   Exported Mar 29, 2018 from SnapGene 4.1.7
            http://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="synthetic"
                     /note="/vntifkey=98"
     misc_feature    complement(8..856)
                     /product="Ampicilin"
                     /label=Ampicilin
                     /note="/vntifkey=21"
     misc_feature    complement(1038..1212)
                     /product="rrnB1 B2 T1 txn terminator"
                     /label=rrnB1 B2 T1 txn terminator
                     /label=rrnB1\B2\T1\txn\terminator
                     /note="/vntifkey=21"
     misc_feature    1113..1129
                     /product="pTR"
                     /label=pTR
                     /note="/vntifkey=21"
     gene            1288..4307
                     /gene="iCreERT2-FFrt-KanR-FFrt_110104"
                     /label=iCreERT2-FFrt-KanR-FFrt_110104
                     /note="/vntifkey=60"
     misc_feature    1288..4307
                     /product="iCreERT2-FFrt-KanR-FFrt_110104"
                     /label=iCreERT2-FFrt-KanR-FFrt_110104
                     /note="/vntifkey=21"
     misc_feature    4384..4684
                     /product="bla txn terminator"
                     /label=bla txn terminator
                     /label=bla\txn\terminator
                     /note="/vntifkey=21"
     misc_feature    4475..4500
                     /product="pTF3"
                     /label=pTF3
                     /note="/vntifkey=21"
     misc_feature    4691..4804
                     /product="rpn txn terminator"
                     /label=rpn txn terminator
                     /label=rpn\txn\terminator
                     /note="/vntifkey=21"
     misc_feature    complement(4976..5779)
                     /product="pUC_ori"
                     /label=pUC_ori
                     /note="/vntifkey=21"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta
     1261 gaaagtatag gaacttctga agtggggatg tccaacctgc tgactgtgca ccaaaacctg
     1321 cctgccctcc ctgtggatgc cacctctgat gaagtcagga agaacctgat ggacatgttc
     1381 agggacaggc aggccttctc tgaacacacc tggaagatgc tcctgtctgt gtgcagatcc
     1441 tgggctgcct ggtgcaagct gaacaacagg aaatggttcc ctgctgaacc tgaggatgtg
     1501 agggactacc tcctgtacct gcaagccaga ggcctggctg tgaagaccat ccaacagcac
     1561 ctgggccagc tcaacatgct gcacaggaga tctggcctgc ctcgcccttc tgactccaat
     1621 gctgtgtccc tggtgatgag gagaatcaga aaggagaatg tggatgctgg ggagagagcc
     1681 aagcaggccc tggcctttga acgcactgac tttgaccaag tcagatccct gatggagaac
     1741 tctgacagat gccaggacat caggaacctg gccttcctgg gcattgccta caacaccctg
     1801 ctgcgcattg ccgaaattgc cagaatcaga gtgaaggaca tctcccgcac cgatggtggg
     1861 agaatgctga tccacattgg caggaccaag accctggtgt ccacagctgg tgtggagaag
     1921 gccctgtccc tgggggttac caagctggtg gagagatgga tctctgtgtc tggtgtggct
     1981 gatgacccca acaactacct gttctgccgg gtcagaaaga atggtgtggc tgccccttct
     2041 gccacctccc aactgtccac ccgggccctg gaagggatct ttgaggccac ccaccgcctg
     2101 atctatggtg ccaaggatga ctctgggcag agatacctgg cctggtctgg ccactctgcc
     2161 agagtgggtg ctgccaggga catggccagg gctggtgtgt ccatccctga aatcatgcag
     2221 gctggtggct ggaccaatgt gaacatagtg atgaactaca tcagaaacct ggactctgag
     2281 actggggcca tggtgaggct gctcgaggat ggggacctcg agccatctgc tggagacatg
     2341 agagctgcca acctttggcc aagcccgctc atgatcaaac gctctaagaa gaacagcctg
     2401 gccttgtccc tgacggccga ccagatggtc agtgccttgt tggatgctga gccccccata
     2461 ctctattccg agtatgatcc taccagaccc ttcagtgaag cttcgatgat gggcttactg
     2521 accaacctgg cagacaggga gctggttcac atgatcaact gggcgaagag ggtgccaggc
     2581 tttgtggatt tgaccctcca tgatcaggtc caccttctag aatgtgcctg gctagagatc
     2641 ctgatgattg gtctcgtctg gcgctccatg gagcacccag tgaagctact gtttgctcct
     2701 aacttgctct tggacaggaa ccagggaaaa tgtgtagagg gcatggtgga gatcttcgac
     2761 atgctgctgg ctacatcatc tcggttccgc atgatgaatc tgcagggaga ggagtttgtg
     2821 tgcctcaaat ctattatttt gcttaattct ggagtgtaca catttctgtc cagcaccctg
     2881 aagtctctgg aagagaagga ccatatccac cgagtcctgg acaagatcac agacactttg
     2941 atccacctga tggccaaggc aggcctgacc ctgcagcagc agcaccagcg gctggcccag
     3001 ctcctcctca tcctctccca catcaggcac atgagtaaca aaggcatgga gcatctgtac
     3061 agcatgaagt gcaagaacgt ggtgcccctc tatgacctgc tgctggaggc ggcggacgcc
     3121 caccgcctac atgcgcccac tagccgtgga ggggcatccg tggaggagac ggaccaaagc
     3181 cacttggcca ctgcgggctc tacttcatcg cattccttgc aaaagtatta catcacgggg
     3241 gaggcagagg gtttccctgc cacagcttga gaagttccta ttccgaagtt cctattctct
     3301 agaaagtata ggaacttccc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
     3361 ccctgcaaag taaactggat ggctttctcg ccgccaagga tctgatggcg caggggatca
     3421 agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     3481 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     3541 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     3601 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     3661 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     3721 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     3781 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     3841 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     3901 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     3961 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     4021 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     4081 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     4141 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     4201 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agaagttcct
     4261 attccgaagt tcctattctc tagaaagtat aggaacttca attaattaaa atgaagtgaa
     4321 gttcctatac tttctagaga ataggaactt ctatagtgag tcgaataagg gcgacacaaa
     4381 atttattcta aatgcataat aaatactgat aacatcttat agtttgtatt atattttgta
     4441 ttatcgttga catgtataat tttgatatca aaaactgatt ttccctttat tattttcgag
     4501 atttattttc ttaattctct ttaacaaact agaaatattg tatatacaaa aaatcataaa
     4561 taatagatga atagtttaat tataggtgtt catcaatcga aaaagcaacg tatcttattt
     4621 aaagtgcgtt gcttttttct catttataag gttaaataat tctcatatat caagcaaagt
     4681 gacaggcgcc cttaaatatt ctgacaaatg ctctttccct aaactccccc cataaaaaaa
     4741 cccgccgaag cgggttttta cgttatttgc ggattaacga ttactcgtta tcagaaccgc
     4801 ccagggggcc cgagcttaag actggccgtc gttttacaac acagaaagag tttgtagaaa
     4861 cgcaaaaagg ccatccgtca ggggccttct gcttagtttg atgcctggca gttccctact
     4921 ctcgccttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4981 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5041 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5101 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5161 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5221 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5281 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5341 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5401 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5461 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5521 gtgggctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5581 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5641 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5701 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gacgcgcgcg taactcacgt
     5761 taagggattt tggtcatgag cttgcgccgt cccgtcaagt cagcgtaatg ctctgctt
//
