LOCUS Kif5aDN_p3E 4499 bp ds-DNA circular 12-DEC-2014 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Insert from/Users/icha/Desktop/kinesins&SUN&nesprins&FAK/Kif5aDN/Kif5a_416 -1033PCR.ape 13 to 1893 COMMENT Backbone from /Users/icha/Desktop/Tol2_GW/tol2kit/211_pDONRP2RP3.gb 2889 to 733 COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 734..749 /label=attB2r /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2615..2703 /vntifkey="21" /label=attL3 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1190..1190 /label=mut /ApEinfo_fwdcolor=#ff3235 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(2607..2614) /label=attB3 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1214..1214 /label=mut(1) /ApEinfo_label=mut /ApEinfo_fwdcolor=#ff243f /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 591..733 /vntifkey="21" /label=attR2 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2357..2357 /label=mut(2) /ApEinfo_label=mut /ApEinfo_fwdcolor=#ff2e40 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2760..2778 /label=M13rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 941..941 /label=mut(3) /ApEinfo_label=mut /ApEinfo_fwdcolor=#ff1512 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 536..553 /label=M13Forw /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2271..2271 /label=mut(4) /ApEinfo_label=mut /ApEinfo_fwdcolor=#ff1812 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 750..2603 /label=Kif5a#1clone real sequence /ApEinfo_fwdcolor=#ffe082 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttCTTGTAC AAAGTGGCTT CCTCTATCGT GGTGCGCATC TCAGAGGAAG 781 AGCGGCAGAA ATACGAGGAA GAGATCCGCA AACTCTACCG CCAGCTGGAC GACAAGGATG 841 ATGAGATCAA TCTCCAGTGT CAGCTGGTGG AGAAGCTGAA GGAGCAGATT CTGGATCAGG 901 ATGAGCTGCT GGCCTCCACC CGCGGCGACA GTGATAAGGT ACAGACGGAG CTGGGTCGTC 961 TGCAGACGGA GAGCGAGAGC GCCAAGGCGG AGGTGCGGGA GGTGCTGCAG GCGCTGGAGG 1021 AGCTGGCCGT CAACTACGAC CAGAAGAGCC AGGAGGTGGA GGAGAAGAGT CTGCACAACA 1081 AACAGCTGGC CGAGCAGCTC AGCCACAAGA TGGCCAACCT GATGGAGCTG GAGGCGGAGC 1141 TAGCGCAGAT GCAGGAAGTG AGCTCACAGC AGAGGAAGCG CATCGCTGAT GTGTTGAACG 1201 GCCTGATGAA GGACCTCAGC GAGTTCAGCA GCATCGTCGG AAACGGAGAA CTCAAGCTAC 1261 CGGTGGAGAT CAGCGGTGCG ATTGAGGAGG AGTTCACCGT GGCACGTCTC TACATCAGCA 1321 AGATTAAATC TGAGGTGAAG TCGATGGTGA AGCGCTGCCG GCAGCTGGAG AACATGCAGC 1381 TGGAGTGCCA TCGCAAGATG GAGGAGACCA GCCGGGAGCT GTCGTCATGC CAACTGCTCA 1441 TCTCACAGCA TGAGGCAAAG ATCCGTTCTC TCACAGAAta catgcagaac gtggagcaga 1501 agaagagaca gctggaggac aactatgatg ctctcagtga agaactctac acactgcaga 1561 actctgagag tcatgtggca gagctggacg gaggagagaa ggcggacaga gaggctgatg 1621 ggaaaaaggc cgctgttcgt ccagctctgc atgcggagtc tcgtcagacg cagctcagcc 1681 gcctgcggga tgaaattaac gagaagcaga gactcatcga cgagctcaca gataagaacc 1741 aggcTCTGGA GTTGGAGCTG GCTCACGTGC GCTCCAGTTT CAGCAATCTG AAGCTGCAGG 1801 ACGACAGCAA GAGCGCCTGT CTGGAGCAGA TGTCGTTTCA ACAGGAGAGA CACGAGCAGT 1861 CCAAACAGGA TCTCAAGGGC CTTGAGGAGA CTGTTGCTCG GGAACTCCAG ACCCTCCACA 1921 ACCTGCGCAA GCTGTTCGTT CAAGACCTCA CGACTCGGGT TAAAAAAAGT TCAGAAATTG 1981 GACCTGATGA TAGTGGGGGG TCTAACACCC AGAAGCAGAA GATTTCCTTT CTTGAGAACA 2041 ACCTGGATCA GCTTACAAAG GTTCATAAAC AGCTGGTACG TGATAATGCA GATCTCCGCT 2101 GTGAGCTTCC GAAGCTGGAG AAACGTCTTC GTTCTACGGC TGAGCGGGTT CGGGCGCTGG 2161 AGACAGCACT GAAGGACGCC AAACAGGGCG CGATGAATGA CCGGCGCCGG TACCAGCAGG 2221 AGGTGGAGCG CATCCGGGAC GCCATGAGGC TGCGTTACCC ACTGCGCCGG GCCAACGCCG 2281 CTCAGATCGC TAAGCCAGTG AGACCCGGGC ATCACGTCAC CGCCACATCG CCCACCAGCT 2341 TCTCCGTCAC ACGCGCAAGC AAACCGGCCA CGTCTTACAG CAATGCCCGA TTCCTGCAGG 2401 ACGAGAAGAT GGAGAACAGC CAGAAGACCA ACACTGTTGG ATACAGCACT GTGAGATCCA 2461 TAGACATACT GAGCTCCTGT CCGCTCGACG TGGAGAACGG AAACAGCACA GATATTAATG 2521 ATAACAGGAG TGATGTTCAC TGCGGCAGCA TGGTGGACGA TGCCTCTCGA GTGTACATCA 2581 TGCAGCAGGA GACTGCAGCC AGTTAAGCAA CTTTattata caaagttggc attataaaaa 2641 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta 2701 tttggagctc catggtagcg ttaacgcggc cgcgatatcc cctatagtga gtcgtattac 2761 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt 2821 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat 2881 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac 2941 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg 3001 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa 3061 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt 3121 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc 3181 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa 3241 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat 3301 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac 3361 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc 3421 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat 3481 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga 3541 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag 3601 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg 3661 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg 3721 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa 3781 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa 3841 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 3901 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 3961 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 4021 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 4081 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 4141 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 4201 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt 4261 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 4321 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 4381 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 4441 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt //