LOCUS       Extended_Data_2_        8763 bp ds-DNA     circular     12-MAY-2017
DEFINITION  pSicoR complete plasmid sequence.
ACCESSION   .
VERSION     .
KEYWORDS    Supplementary X - sgLenti orthogonal
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 28, 2016 from SnapGene 3.1.4
            http://www.snapgene.com
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    844..1024
                     /note="HIV-1_5_LTR"
                     /label=HIV-1_5_LTR
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2629..2943
                     /note="mouse U6 promoter"
                     /label=mouse U6 promoter
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2948..2954
                     /note="AarI site"
                     /label=AarI site
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3010..3017
                     /note="AscI kill site"
                     /label=AscI kill site
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3055..3061
                     /note="AarI site(1)"
                     /label=AarI site(1)
                     /ApEinfo_label="AarI site"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3066..3158
                     /note="SpCas9-TRACR"
                     /label=SpCas9 TRACR
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(join(3165..3359,3361..3362))
                     /note="250nt ORF chicken beta globin"
                     /label=250nt ORF chicken beta globin
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3369..3638
                     /note="human U6 promoter"
                     /label=human U6 promoter
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3643..3648
                     /note="BfuAI site"
                     /label=BfuA1 site
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3698..3705
                     /note="AscI kill site(1)"
                     /label=AscI kill site(1)
                     /ApEinfo_label="AscI kill site"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3751..3756
                     /note="BfuAI site"
                     /label=BfuA1 site(1)
                     /ApEinfo_label="BfuA1 site"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3761..3841
                     /note="SaCas9 TRACR"
                     /label=SaCas9 TRACR
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3921..4152
                     /note="Short EF1alpha promoter"
                     /label=EFS promoter
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4163..4822
                     /note="Puromycin resistance"
                     /label=Puromycin resistance
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            4835..5542
                     /note="mCherry"
                     /label=mCherry
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5543..5572
                     /note="3x NLS"
                     /label=3x NLS
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5654..6241
                     /note="WPRE"
                     /label=WPRE
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6769..6949
                     /note="truncHIV-1_3_LTR"
                     /label=truncHIV-1_3_LTR
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     1501 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gcgctgatct tcagacctgg
     1561 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1621 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1681 agcagtggga ataggagctt tgttccttgg gttcttcgga gcagcaggaa gcactatggg
     1741 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1801 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1861 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1921 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1981 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     2041 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     2101 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     2161 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     2221 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     2281 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     2341 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2401 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     2461 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2521 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2581 acagggacag cagagatcca gtttggttag taccgggccc gctctagaga tccgacgcgc
     2641 catctctagg cccgcgccgg ccccctcgca cggacttgtg ggagaagctc ggctactccc
     2701 ctgccccggt taatttgcat ataatatttc ctagtaacta tagaggctta atgtgcgata
     2761 aaagacagat aatctgttct ttttaatact agctacattt tacatgatag gcttggattt
     2821 ctataacttc gtatagcata cattatacga agttataaac agcacaaaag gaaactcacc
     2881 ctaactgtaa agtaattgtg tgttttgaga ctataagtat cccttggaga accaccttgt
     2941 tggagacgca ggtgtcactg gcgctgttgg tgccggcacc tgacacagca gaggatgcag
     3001 agcccaaggg gcgcgccaaa gccgaaggag ccacccagca cgcaagccag gaaccacctg
     3061 cgatcgttta agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta
     3121 tcaacttgaa aaagtggcac cgagtcggtg ctttttttgc atgcacagca tccccaaagg
     3181 aggtgagcac tttcttgccg tgggcgcgga ccatggggtt gccaaggatg gcagtggggc
     3241 tggagaggtt cccaaaggac gcaaagaacc tctgggtcca ggggtagacg atcagcagcc
     3301 tggccagggc ttcggcccca cattcggcca cattgacctt gccccagagg ccggtgatga
     3361 gccctaggta gcggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca
     3421 tatacgatac aaggtctgtt agagagataa ttagaattaa tttgactgta aacacaaaga
     3481 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     3541 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     3601 tggctttata tatcttgtgg aaaggacgaa acaccgtgag acgcaggtac ttccccagca
     3661 ccagcgtctt cccttgagtg acagctagga gttgtgaggc gcgccgcagc agcagcagca
     3721 agcgcctaag agagatggct cggcacatgt acctgcgatc gttttagtac tctggaaaca
     3781 gaatctacta aaacaaggca aaatgccgtg tttatctcgt caacttgttg gcgagatttt
     3841 tgcnnnnnnn nnnnnnnnnn nnnagatcgg aagagcacac gtctgaactc cagtcacgcg
     3901 tggataaccg tattaccgcc atgcatgtgc ccgtcagtgg gcagagcgca catcgcccac
     3961 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     4021 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     4081 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     4141 cagaacagtg agctagccca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     4201 cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     4261 gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     4321 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     4381 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     4441 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     4501 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     4561 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     4621 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     4681 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     4741 gacccgcaag cccggtgccg gatcgggaga gggcagagga agtctgctaa catgcggtga
     4801 cgtcgaggag aatcctggcc caccggtcgc caccatggtg agcaagggcg aggaggataa
     4861 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     4921 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     4981 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     5041 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     5101 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     5161 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     5221 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     5281 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     5341 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     5401 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     5461 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     5521 cggcatggac gagctgtaca agggagctga tccaaaaaag aagagaaagg tatagttaat
     5581 taagtcgagg gacctaacat gtttaagggt tccggttcca ctaggtacaa ttcgatatca
     5641 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5701 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5761 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5821 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5881 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5941 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6001 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     6061 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6121 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6181 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6241 atcgataccg tcgacctcga tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     6301 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     6361 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6421 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     6481 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
     6541 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
     6601 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
     6661 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
     6721 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
     6781 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6841 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6901 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag catgtgagca
     6961 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7021 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7081 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7141 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7201 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7261 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7321 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7381 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7441 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7501 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7561 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7621 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7681 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7741 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7801 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7861 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7921 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7981 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8041 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8101 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8161 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8221 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8281 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8341 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8401 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8461 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8521 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8581 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8641 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8701 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8761 gac     
//
