LOCUS       pDONR/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
                     /note="recognized as attL1"
     misc_recomb     complement(1652..1750)
                     /label=attL2
                     /note="attL2 (c)"
                     /note="recognized as attL2"
     misc_signal     complement(1765..1784)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1792..1808
                     /note="M13 Reverse priming site"
     gene            1921..2730
                     /note="Kanamycin resistance gene"
     rep_origin      2851..3524
                     /note="pUC origin"
     source          669..1651
                     /dnas_title="pENTR4_ELAVL1_NM_001419.2"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.2"
     gene            669..1651
                     /gene="ELAVL1"
                     /gene_synonym="ELAV1; Hua; HUR; MelG"
                     /note="ELAV like RNA binding protein 1"
                     /db_xref="GeneID:1994"
                     /db_xref="HGNC:HGNC:3312"
                     /db_xref="HPRD:16025"
                     /db_xref="MIM:603466"
     exon            669..842
                     /gene="ELAVL1"
                     /gene_synonym="ELAV1; Hua; HUR; MelG"
                     /inference="alignment:Splign:1.39.8"
     CDS             669..1651
                     /gene="ELAVL1"
                     /gene_synonym="ELAV1; Hua; HUR; MelG"
                     /note="Hu antigen R; embryonic lethal, abnormal vision,
                     drosophila, homolog-like 1; hu-antigen R; ELAV (embryonic
                     lethal, abnormal vision, Drosophila)-like 1 (Hu antigen
                     R)"
                     /codon_start=1
                     /product="ELAV-like protein 1"
                     /protein_id="NP_001410.2"
                     /db_xref="GI:38201714"
                     /db_xref="CCDS:CCDS12193.1"
                     /db_xref="GeneID:1994"
                     /db_xref="HGNC:HGNC:3312"
                     /db_xref="HPRD:16025"
                     /db_xref="MIM:603466"
                     /translation="MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIG
                     EVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEV
                     IKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEA
                     EEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRF
                     RFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVI
                     RDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK"
     misc_feature    674..676
                     /gene="ELAVL1"
                     /gene_synonym="ELAV1; Hua; HUR; MelG"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N-acetylserine; propagated from
                     UniProtKB/Swiss-Prot (Q15717.2); acetylation site"
     misc_feature    674..676
                     /gene="ELAVL1"
                     /gene_synonym="ELAV1; Hua; HUR; MelG"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine; propagated from
                     UniProtKB/Swiss-Prot (Q15717.2); phosphorylation site"
     exon            843..946
                     /gene="ELAVL1"
                     /gene_synonym="ELAV1; Hua; HUR; MelG"
                     /inference="alignment:Splign:1.39.8"
     exon            947..1100
                     /gene="ELAVL1"
                     /gene_synonym="ELAV1; Hua; HUR; MelG"
                     /inference="alignment:Splign:1.39.8"
     PCR_primer      669..699
                     /primer="ATGTCTAATGGTTATGAAGACCACATGGC"
                     /set="Set 1"
                     /pair="Pair 1"
                     /current=1
     PCR_product     669..1651
                     /pair="Pair 1"
                     /set="Set 1"
                     /current=1
                     /score="0.000"
     PCR_frame       669..1651
                     /note="Reading Frame"
                     /set="Set 1"
     exon            1101..1326
                     /gene="ELAVL1"
                     /gene_synonym="ELAV1; Hua; HUR; MelG"
                     /inference="alignment:Splign:1.39.8"
     insert          669..1651
                     /source="ELAVL1_NM_001419.2"
                     /type="BP AttB Insert"
     PCR_frame       669..1651
                     /note="Reading Frame"
                     /set="Set 2"
     misc_feature    1274..1276
                     /gene="ELAVL1"
                     /gene_synonym="ELAV1; Hua; HUR; MelG"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine; propagated from
                     UniProtKB/Swiss-Prot (Q15717.2); phosphorylation site"
     misc_feature    1319..1321
                     /gene="ELAVL1"
                     /gene_synonym="ELAV1; Hua; HUR; MelG"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Omega-N-methylated arginine, by CARM1; propagated
                     from UniProtKB/Swiss-Prot (Q15717.2); methylation site"
     exon            1327..1651
                     /gene="ELAVL1"
                     /gene_synonym="ELAV1; Hua; HUR; MelG"
                     /inference="alignment:Splign:1.39.8"
     PCR_primer      complement(1629..1651)
                     /primer="TTATTTGTGGGACTTGTTGGTTT"
                     /set="Set 1"
                     /pair="Pair 1"
                     /current=1
     PCR_primer      669..699
                     /primer="ATGTCTAATGGTTATGAAGACCACATGGC"
                     /set="Set 1"
                     /pair="Pair 1"
                     /current=1
     PCR_primer      659..693
                     /primer="AAAGCAGGCTATGTCTAATGGTTATGAAGACCA"
                     /set="Set 2"
                     /pair="Pair 1"
                     /current=1
                     /original="ACAAGTTTGTACAAAAAAGCAGGCTATGTCTAATGGTTATGAAGACC
                     A"
     PCR_primer      complement(1629..1651)
                     /primer="TTATTTGTGGGACTTGTTGGTTT"
                     /set="Set 1"
                     /pair="Pair 1"
                     /current=1
     PCR_primer      complement(1629..1661)
                     /primer="AAAGCTGGGTTTATTTGTGGGACTTGTTGGTTT"
                     /set="Set 2"
                     /pair="Pair 1"
                     /current=1
                     /original="ACCACTTTGTACAAGAAAGCTGGGTTTATTTGTGGGACTTGTTGGTT
                     T"
     PCR_product     669..1651
                     /pair="Pair 1"
                     /set="Set 1"
                     /current=1
                     /score="0.000"
     PCR_product     complement(659..1661)
                     /pair="Pair 1"
                     /set="Set 2"
                     /current=1
                     /score="0.000"
     vector          join(1662..3527,1..658)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     clone           659..1661
                     /source="ELAVL1_NM_001419.2 AttB Insert"
                     /type="Expression Clone"
ORIGIN      
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTTC atgtctaatg gttatgaaga ccacatggcc gaagactgca ggggtgacat
      721 cgggagaacg aatttgatcg tcaactacct ccctcagaac atgacccagg atgagttacg
      781 aagcctgttc agcagcattg gtgaagttga atctgcaaaa cttattcggg ataaagtagc
      841 aggacacagc ttgggctatg gctttgtgaa ctacgtgacc gcgaaggatg cagagagagc
      901 gatcaacacg ctgaacggct tgaggctcca gtcaaaaacc attaaggtgt cgtatgctcg
      961 cccgagctca gaggtgatca aagacgccaa cttgtacatc agcgggctcc cgcggaccat
     1021 gacccagaag gacgtagaag acatgttctc tcggtttggg cggatcatca actcgcgggt
     1081 cctcgtggat cagactacag gtttgtccag aggggttgcg tttatccggt ttgacaaacg
     1141 gtcggaggca gaagaggcaa ttaccagttt caatggtcat aaacccccag gttcctctga
     1201 gcccatcaca gtgaagtttg cagccaaccc caaccagaac aaaaacgtgg cactcctctc
     1261 gcagctgtac cactcgccag cgcgacggtt cggaggcccc gttcaccacc aggcgcagag
     1321 attcaggttc tcccccatgg gcgtcgatca catgagcggg ctctctggcg tcaacgtgcc
     1381 aggaaacgcc tcctccggct ggtgcatttt catctacaac ctggggcagg atgccgacga
     1441 ggggatcctc tggcagatgt ttgggccgtt tggtgccgtc accaatgtga aagtgatccg
     1501 cgacttcaac accaacaagt gcaaagggtt tggctttgtg accatgacaa actatgaaga
     1561 agccgcgatg gccatagcca gcctgaacgg ctaccgcctg ggggacaaaa tcttacaggt
     1621 ttccttcaaa accaacaagt cccacaaata aACCCAGCTT TCTTGTACAA AGTTGGCATT
     1681 ATAAGAAAGC ATTGCTTATC AATTTGTTGC AACGAACAGG TCACTATCAG TCAAAATAAA
     1741 ATCATTATTT GCCATCCAGC TGATATCCCC TATAGTGAGT CGTATTACAT GGTCATAGCT
     1801 GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA
     1861 ATAATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC AAGGGGTGTT
     1921 ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT
     1981 TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGC
     2041 TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC
     2101 AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG
     2161 ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC
     2221 GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT
     2281 GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC
     2341 AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT
     2401 GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG
     2461 CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT
     2521 AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC
     2581 GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA
     2641 TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG
     2701 TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG TTAATTGGTT GTAACACTGG
     2761 CAGAGCATTA CGCTGACTTG ACGGGACGGC GCAAGCTCAT GACCAAAATC CCTTAACGTG
     2821 AGTTACGCGT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA
     2881 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG
     2941 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC
     3001 AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG
     3061 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC
     3121 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG
     3181 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC
     3241 ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA
     3301 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT
     3361 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG
     3421 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG
     3481 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTT
//

