LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" /note="recognized as attL1" misc_recomb complement(1613..1711) /label=attL2 /note="attL2 (c)" /note="recognized as attL2" misc_signal complement(1726..1745) /note="T7 Promoter/priming site (c)" primer_bind 1753..1769 /note="M13 Reverse priming site" gene 1882..2691 /note="Kanamycin resistance gene" rep_origin 2812..3485 /note="pUC origin" source 671..1612 /dnas_title="pENTR4_PRKRA_NM_003690" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /chromosome="2" /map="2q31.2" gene 671..1612 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /note="protein kinase, interferon-inducible double stranded RNA dependent activator" /db_xref="GeneID:8575" /db_xref="HGNC:HGNC:9438" /db_xref="HPRD:04573" /db_xref="MIM:603424" exon 671..735 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /inference="alignment:Splign:1.39.8" CDS 671..1612 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /note="isoform 1 is encoded by transcript variant 1; protein activator of the interferon-induced protein kinase; interferon-inducible double stranded RNA-dependent protein kinase activator A; protein kinase, interferon-inducible double stranded RNA-dependent activator; PKR-associated protein X; PKR-associating protein X; protein kinase, interferon-inducible double-stranded RNA-dependent activator; interferon-inducible double-stranded RNA-dependent protein kinase activator A" /codon_start=1 /product="interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 1" /protein_id="NP_003681.1" /db_xref="GI:4505581" /db_xref="CCDS:CCDS2279.1" /db_xref="GeneID:8575" /db_xref="HGNC:HGNC:9438" /db_xref="HPRD:04573" /db_xref="MIM:603424" /translation="MSQSRHRAEAPPLEREDSGTFSLGKMITAKPGKTPIQVLHEYGM KTKNIPVYECERSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKAN ASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYT TICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHSLGCTWHSL RNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIDELSANGQYQCLAEL STSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERK" misc_feature 671..979 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (O75569.1); Region: Sufficient for self-association and interaction with TARBP2" misc_feature 722..724 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /experiment="experimental evidence, no additional details recorded" /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot (O75569.1); phosphorylation site" exon 736..905 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /inference="alignment:Splign:1.39.8" exon 906..987 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /inference="alignment:Splign:1.39.8" PCR_primer 671..690 /primer="ATGTCCCAGAGCAGGCACCG" /set="Set 1" /pair="Pair 1" /current=1 PCR_product 671..1612 /pair="Pair 1" /set="Set 1" /current=1 /score="0.000" PCR_frame 671..1612 /note="Reading Frame" /set="Set 1" misc_feature 974..1255 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (O75569.1); Region: Sufficient for self-association and interaction with TARBP2" exon 988..1066 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /inference="alignment:Splign:1.39.8" exon 1067..1184 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /inference="alignment:Splign:1.39.8" insert 671..1612 /source="PRKRA_NM_003690" /type="BP AttB Insert" PCR_frame 671..1612 /note="Reading Frame" /set="Set 2" misc_feature 1253..1609 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (O75569.1); Region: Sufficient for self-association and interaction with TARBP2" misc_feature 1406..1408 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /experiment="experimental evidence, no additional details recorded" /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot (O75569.1); phosphorylation site" exon 1185..1279 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /inference="alignment:Splign:1.39.8" exon 1280..1454 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /inference="alignment:Splign:1.39.8" exon 1455..1612 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /inference="alignment:Splign:1.39.8" misc_feature 1529..1531 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /experiment="experimental evidence, no additional details recorded" /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot (O75569.1); phosphorylation site" STS 1483..1565 /gene="PRKRA" /gene_synonym="DYT16; HSD14; PACT; RAX" /standard_name="D2S1579E" /db_xref="UniSTS:150663" PCR_primer complement(1575..1612) /primer="TTACTTTCTTTCTGCTATTATCTUUAAAUACUGCAAAG" /set="Set 1" /pair="Pair 1" /current=1 PCR_primer 671..690 /primer="ATGTCCCAGAGCAGGCACCG" /set="Set 1" /pair="Pair 1" /current=1 PCR_primer 659..690 /primer="AAAGCAGGCTATGTCCCAGAGCAGGCACCG" /set="Set 2" /pair="Pair 1" /current=1 /original="ACAAGTTTGTACAAAAAAGCAGGCTATGTCCCAGAGCAGGCACCG" PCR_primer complement(1575..1612) /primer="TTACTTTCTTTCTGCTATTATCTUUAAAUACUGCAAAG" /set="Set 1" /pair="Pair 1" /current=1 PCR_primer complement(1590..1622) /primer="AAAGCTGGGTTTACTTTCTTTCTGCTATTATCT" /set="Set 2" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTACTTTCTTTCTGCTATTATC T" PCR_product 671..1612 /pair="Pair 1" /set="Set 1" /current=1 /score="0.000" PCR_product complement(659..1622) /pair="Pair 1" /set="Set 2" /current=1 /score="0.000" vector join(1623..3488,1..658) /source="pDONR%99221" /type="Donor Vector" clone 659..1622 /source="PRKRA_NM_003690 AttB Insert" /type="Expression Clone" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTTC atgtcccaga gcaggcaccg cgccgaggcc ccgccgctgg agcgcgagga 721 cagtgggacc ttcagtttgg ggaagatgat aacagctaag ccagggaaaa caccgattca 781 ggtattacac gaatacggca tgaagaccaa gaacatccca gtttatgaat gtgaaagatc 841 tgatgtgcaa atacacgtgc ccactttcac cttcagagta accgttggtg acataacctg 901 cacaggtgaa ggtacaagta agaagctggc gaaacataga gctgcagagg ctgccataaa 961 cattttgaaa gccaatgcaa gtatttgctt tgcagttcct gaccccttaa tgcctgaccc 1021 ttccaagcaa ccaaagaacc agcttaatcc tattggttca ttacaggaat tggctattca 1081 tcatggctgg agacttcctg aatataccct ttcccaggag ggaggacctg ctcataagag 1141 agaatatact acaatttgca ggctagagtc atttatggaa actggaaagg gggcatcaaa 1201 aaagcaagcc aaaaggaatg ctgctgagaa atttcttgcc aaatttagta atatttctcc 1261 agagaaccac atttctttaa caaatgtagt aggacattct ttaggatgta cttggcattc 1321 cttgaggaat tctcctggtg aaaagatcaa cttactgaaa agaagcctcc ttagtattcc 1381 aaatacagat tacatccagc tgcttagtga aattgccaag gaacaaggtt ttaatataac 1441 atatttggat atagatgaac tgagcgccaa tggacaatat caatgtcttg ctgaactgtc 1501 caccagcccc atcacagtct gtcatggctc cggtatctcc tgtggcaatg cacaaagtga 1561 tgcagctcac aatgctttgc agtatttaaa gataatagca gaaagaaagt aaACCCAGCT 1621 TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG 1681 GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCC CTATAGTGAG 1741 TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA AAATCTCTGA 1801 TGTTACATTG CACAAGATAA AATAATATCA TCATGAACAA TAAAACTGTC TGCTTACATA 1861 AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG GCCGCGATTA 1921 AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA 1981 TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA 2041 CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG 2101 ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG 2161 TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT 2221 TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT 2281 GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA 2341 ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT 2401 GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC AGTCGTCACT 2461 CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT 2521 GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAACTGC 2581 CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG TATTGATAAT 2641 CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA ATCAGAATTG 2701 GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT GACGGGACGG CGCAAGCTCA 2761 TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT GAGCGTCAGA CCCCGTAGAA 2821 AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA 2881 AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT 2941 CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG 3001 TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC 3061 CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA 3121 CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC 3181 AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC 3241 GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA 3301 GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG 3361 TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA 3421 TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT 3481 CACATGTT //