LOCUS       Exported                8175 bp ds-DNA     circular SYN 20-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2017 from SnapGene 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3301..3942
                     /label=PRKRA_NM_003690
     misc_feature    3292..3297
                     /label=BamHI
                     /note="BamHI"
     misc_feature    3301..3942
                     /label=Pair 1
     misc_feature    3301..3942
                     /label=Pair 1
     misc_feature    complement(3301..3942)
                     /label=Pair 1
     gene            3301..3942
                     /label=PRKRA
                     /note="protein kinase, interferon-inducible double stranded
                     RNA dependent activator"
     CDS             3301..3942
                     /codon_start=1
                     /label=PRKRA
                     /note="isoform 1 is encoded by transcript variant 1; 
                     protein activator of the interferon-induced protein kinase;
                     interferon-inducible double stranded RNA-dependent protein 
                     kinase activator A; protein kinase, interferon-inducible 
                     double stranded RNA-dependent activator; PKR-associated 
                     protein X; PKR-associating protein X; protein kinase, 
                     interferon-inducible double-stranded RNA-dependent 
                     activator; interferon-inducible double-stranded 
                     RNA-dependent protein kinase activator A"
                     /translation="KANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPE
                     YTLSQEGGPAHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISL
                     TNVVGHSLGCTWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDID
                     ELSANGQYQCLAELSTSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERK"
     misc_feature    3301..3942
                     /label=Reading Frame
                     /note="Reading Frame"
     misc_feature    3301..3942
                     /label=PRKRA_NM_003690
     misc_feature    3301..3942
                     /label=Reading Frame
                     /note="Reading Frame"
     misc_feature    3301..3942
                     /label=PRKRA_coding_sequence
                     /note="PRKRA_coding_sequence"
     exon            3301..3320
                     /label=PRKRA
     misc_feature    3301..3312
                     /label=PRKRA
                     /note="propagated from UniProtKB/Swiss-Prot (O75569.1); 
                     Region: Sufficient for self-association and interaction 
                     with TARBP2"
     misc_feature    3307..3588
                     /label=PRKRA
                     /note="propagated from UniProtKB/Swiss-Prot (O75569.1); 
                     Region: Sufficient for self-association and interaction 
                     with TARBP2"
     exon            3321..3399
                     /label=PRKRA
     exon            3400..3517
                     /label=PRKRA
     exon            3518..3612
                     /label=PRKRA
     misc_feature    3586..3942
                     /label=PRKRA
                     /note="propagated from UniProtKB/Swiss-Prot (O75569.1); 
                     Region: Sufficient for self-association and interaction 
                     with TARBP2"
     exon            3613..3787
                     /label=PRKRA
     misc_feature    3739..3741
                     /label=PRKRA
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot 
                     (O75569.1); phosphorylation site"
     exon            3788..3942
                     /label=PRKRA
     STS             3816..3898
                     /label=PRKRA
     misc_feature    3862..3864
                     /label=PRKRA
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot 
                     (O75569.1); phosphorylation site"
     primer_bind     complement(3908..3942)
                     /label=Set 1 - Pair 1 (4)
     primer_bind     complement(3908..3942)
                     /label=Set 1 - Pair 1 (3)
     primer_bind     complement(3908..3942)
                     /label=Set 1 - Pair 1 (2)
     primer_bind     complement(3908..3942)
                     /label=Set 1 - Pair 1
     primer_bind     complement(3923..3942)
                     /label=Set 2 - Pair 1 (2)
     primer_bind     complement(3923..3942)
                     /label=Set 2 - Pair 1
     misc_feature    3943..3948
                     /label=MluI
                     /note="MluI"
     misc_feature    3958..3963
                     /label=XhoI
                     /note="XhoI"
     misc_feature    3961..3990
                     /label=Myc-Tag
                     /note="Myc-Tag"
     misc_feature    4009..4032
                     /label=DDK-Tag
                     /note="DDK-Tag"
     misc_feature    4036..4038
                     /label=STOP (TAA)
                     /note="STOP (TAA)"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
     1561 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1621 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1681 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1741 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1801 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1861 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1921 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1981 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2041 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2101 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2161 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2221 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2281 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2341 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2401 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2461 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2521 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2581 cagagatcca gtttggttag taccgggccc gctctagaca tgtccaatat gaccgccatg
     2641 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     2701 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2761 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2821 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2881 tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc
     2941 ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt
     3001 attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata
     3061 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     3121 ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca
     3181 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     3241 tcagaatttt gtaatacgac tcactatagg gcggccggga attcgtcgac tggatccatg
     3301 aaagccaatg caagtatttg ctttgcagtt cctgacccct taatgcctga cccttccaag
     3361 caaccaaaga accagcttaa tcctattggt tcattacagg aattggctat tcatcatggc
     3421 tggagacttc ctgaatatac cctttcccag gagggaggac ctgctcataa gagagaatat
     3481 actacaattt gcaggctaga gtcatttatg gaaactggaa agggggcatc aaaaaagcaa
     3541 gccaaaagga atgctgctga gaaatttctt gccaaattta gtaatatttc tccagagaac
     3601 cacatttctt taacaaatgt agtaggacat tctttaggat gtacttggca ttccttgagg
     3661 aattctcctg gtgaaaagat caacttactg aaaagaagcc tccttagtat tccaaataca
     3721 gattacatcc agctgcttag tgaaattgcc aaggaacaag gttttaatat aacatatttg
     3781 gatatagatg aactgagcgc caatggacaa tatcaatgtc ttgctgaact gtccaccagc
     3841 cccatcacag tctgtcatgg ctccggtatc tcctgtggca atgcacaaag tgatgcagct
     3901 cacaatgctt tgcagtattt aaagataata gcagaaagaa agacgcgtac gcggccgctc
     3961 gagcagaaac tcatctcaga agaggatctg gcagcaaatg atatcctgga ttacaaggat
     4021 gacgacgata aggtttaaac ctaggcgtag cggccgcaaa ttccgcccct ctccctcccc
     4081 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     4141 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     4201 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     4261 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     4321 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     4381 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     4441 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     4501 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     4561 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     4621 ttgaaaaaca cgatgataat atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     4681 acgacgtccc ccgggcagta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     4741 gccacaccgt cgatccagac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     4801 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     4861 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     4921 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     4981 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     5041 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     5101 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     5161 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     5221 cccgcaagcc cggtgcctga tgtacaagta ggattcgtcg agggacctaa taacttcgta
     5281 tagcatacat tatacgaagt tatacatgtt taagggttcc ggttccacta ggtacaattc
     5341 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5401 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     5461 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     5521 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     5581 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     5641 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5701 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     5761 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5821 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5881 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5941 tccccgcatc gataccgtcg acctcgatcg agacctagaa aaacatggag caatcacaag
     6001 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     6061 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     6121 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6181 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     6241 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     6301 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     6361 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     6421 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     6481 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6541 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6601 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     6661 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6721 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6781 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6841 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6901 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6961 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7021 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7081 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7141 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7201 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7261 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7321 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7381 gattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
     7441 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     7501 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     7561 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata
     7621 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     7681 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     7741 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     7801 tcaccgtctt tcattgccat acggaactcc ggatgagcat tcatcaggcg ggcaagaatg
     7861 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     7921 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     7981 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     8041 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8101 tacatatttg aatgtattta gaaaaataaa caaatagggg tcccgcgcac atttccccga
     8161 aaagtgccac ctgac
//
