LOCUS       Exported                7343 bp ds-DNA     linear   SYN 20-DEC-2017
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCfB4586 (pIntB-Ura-TPex20-TLip2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2017 from SnapGene 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    14..513
                     /label=IntB_up
     terminator      complement(527..839)
                     /label=TPex20
     misc_feature    840..868
                     /label=USER cloning site
     terminator      869..1805
                     /label=T lip2
     misc_feature    1828..1861
                     /label=loxP
     promoter        1862..2863
                     /label=PrExp1
     CDS             2864..3718
                     /label=Ura3
     terminator      3719..3951
                     /label=Tcyc1
     misc_feature    3952..3985
                     /label=loxP
     misc_feature    4011..4496
                     /label=IntB_down
     misc_feature    4954..5814
                     /label=AmpR
     misc_feature    complement(5915..6558)
                     /label=pUC ORI
ORIGIN
        1 gaatgcgtgc gatcccacag ttctcactca gatcatggag actctaacct tgagacatca
       61 attatcagct ctcgaggata atgttagtgc agttccagga ctcattgtgc aactgtcacc
      121 acggcatttt gggtctgttc ttttgaagta cagaaatatc ctcattgttg gtatactttg
      181 ggactttttc ttgttacaga agaataaaaa aacctcgact gatgtactaa ttacatggtt
      241 aacatcccca aggtcaaagt acagatattg taccgacttc tgaaatttgt gggatccaca
      301 cacggacctc tgcgatgata caatatcatg gttccatggt ctcttgaatc acaccactca
      361 ataataaaca ccagctcttt caatccaact tagctttctt ccacttgaac ataggccatc
      421 cccccttctt tctattttac ataaatagca agatccttac ccctacatgt ctcataacac
      481 aatctcaact tgacttccca taagaagttc actacgtgca acgcttacgc aactaacatg
      541 aatgaatacg atatacatca aagactatga tacgcagtat tgcacactgt acgagtaagg
      601 gcactagcca ctgcactcaa gtgaaaccgt tgcccgggta cgagtatgag tatgtacagt
      661 atgtttagta ttgtacttgg acaatgcttg tatcgtacat tctcaagtgt caaacataaa
      721 tatccgttgc tatatcctcg caccaccacg tagctcgcta tatccctgtg ttgaatccat
      781 ccatcttgga ttgccaattg tgcacacaga accgggcact cacttcccca tccacacttc
      841 aacggaatgc gtgcgatcgc gtgcattcct tctgttcgga atcaacctca aggttaacgg
      901 ccacgatccc ctcgttgtta ctcttggtca gcccattgtc ggtaacgctg gctttgctaa
      961 ctgggtcgat aaactcttct ttggccagga gaaccccgat gtctccaagg tgtccaaaga
     1021 ccgaaagctc taccgaatca cccaccgagg agatatcgtc cctcaagtgc ccttctggga
     1081 cggttaccag cactgctctg gtgaggtctt tattgactgg cccctgatcc accctcctct
     1141 ctccaacgtt gtcatgtgcc agggccagag caataaacag tgctctgccg gtaacactct
     1201 gctccagcag gtcaatgtga ttggaaacca tctgcagtac ttcgtcaccg agggtgtctg
     1261 tggtatctaa gctatttatc actctttaca acttctacct caactatcta ctttaataaa
     1321 tgaatatcgt ttattctcta tgattactgt atatgcgttc ctctaagaca aatcgaaacc
     1381 agcatgcgat cgaatggcat acaaaagttt cttccgaagt tgatcaatgt cctgatagtc
     1441 aggcagcttg agaagattga cacaggtgga ggccgtaggg aaccgatcaa cctgtctacc
     1501 agcgttacga atggcaaatg acgggttcaa agccttgaat ccttgcaatg gtgccttgga
     1561 tactgatgtc acaaacttaa gaagcagccg cttgtcctct tcctcgaaac tctcaaacac
     1621 agtccagaag tcctttatag tttgatctgt atccagatag cctccgtaat tggtgtgtgt
     1681 cttcaaatcc cagacgtcca cattggcatg tcctccactg ataagcattt gaagttcatc
     1741 tgcgttgaac attgagaccc acgaagggtc aatgagctgg tatagaccgc ccaagaatgc
     1801 atctgagaac agctgaagct tcgtacgata acttcgtata atgtatgcta tacgaagtta
     1861 taaggagttt ggcgcccgtt ttttcgagcc ccacacgttt cggtgagtat gagcggcggc
     1921 agattcgagc gtttccggtt tccgcggctg gacgagagcc catgatgggg gctcccacca
     1981 ccagcaatca gggccctgat tacacaccca cctgtaatgt catgctgttc atcgtggtta
     2041 atgctgctgt gtgctgtgtg tgtgtgttgt ttggcgctca ttgttgcgtt atgcagcgta
     2101 caccacaata ttggaagctt attagccttt ctattttttc gtttgcaagg cttaacaaca
     2161 ttgctgtgga gagggatggg gatatggagg ccgctggagg gagtcggaga ggcgttttgg
     2221 agcggcttgg cctggcgccc agctcgcgaa acgcacctag gaccctttgg cacgccgaaa
     2281 tgtgccactt ttcagtctag taacgcctta cctacgtcat tccatgcatg catgtttgcg
     2341 ccttttttcc cttgcccttg atcgccacac agtacagtgc actgtacagt ggaggttttg
     2401 ggggggtctt agatgggagc taaaagcggc ctagcggtac actagtggga ttgtatggag
     2461 tggcatggag cctaggtgga gcctgacagg acgcacgacc ggctagcccg tgacagacga
     2521 tgggtggctc ctgttgtcca ccgcgtacaa atgtttgggc caaagtcttg tcagccttgc
     2581 ttgcgaacct aattcccaat tttgtcactt cgcaccccca ttgatcgagc cctaacccct
     2641 gcccatcagg caatccaatt aagctcgcat tgtctgcctt gtttagtttg gctcctgccc
     2701 gtttcggcgt ccacttgcac aaacacaaac aagcattata tataaggctc gtctctccct
     2761 cccaaccaca ctcacttttt tgcccgtctt cccttgctaa cacaaaagtc aagaacacaa
     2821 acaaccaccc caaccccctt acacacaaga catatctaca gcaatgccct cctacgaggc
     2881 ccgagccaac gtccacaagt ccgccttcgc cgcccgagtc ctgaagctgg tcgccgccaa
     2941 gaagaccaac ctgtgcgcct ccctggacgt caccaccacc aaggagctga tcgagctggc
     3001 cgacaaggtc ggcccctacg tctgcatgat caagacccac atcgacatca tcgacgactt
     3061 cacctacgcc ggcaccgtcc tgcccctgaa ggagctggcc ctgaagcacg gcttcttcct
     3121 gttcgaggac cgaaagttcg ccgacatcgg caacaccgtc aagcaccagt accgatgcca
     3181 ccgaatcgcc gagtggtccg acatcaccaa cgcccacggc gtccccggca ccggcatcat
     3241 cgccggcctg cgagccggcg ccgaggagac cgtctccgag cagaagaagg aggacgtctc
     3301 cgactacgag aactcccagt acaaggagtt cctggtcccc tcccccaacg agaagctggc
     3361 ccgaggcctg ctgatgctgg ccgagctgtc ctgcaagggc tccctggcca ccggcgagta
     3421 ctccaagcag accatcgagc tggcccgatc cgaccccgag ttcgtcgtcg gcttcatcgc
     3481 ccagaaccga cccaagggcg actccgagga ctggctgatc ctgacccccg gcgtcggcct
     3541 ggacgacaag ggcgacgccc tgggccagca gtaccgaacc gtcgaggacg tcatgtccac
     3601 cggcaccgac atcatcatcg tcggccgagg cctgtacggc cagaaccgag accccatcga
     3661 ggaggccaag cgataccaga aggccggctg ggaggcctac cagaagatca actgctagtc
     3721 atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa
     3781 aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta
     3841 gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac
     3901 gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc gataacttcg
     3961 tataatgtat gctatacgaa gttatgtgat atcagatcca ctagtggcct acctgctcct
     4021 gcacctaagt tcgtcaaaat cggttttact cagagaatca acaaccctac caactgtaca
     4081 tactgctaac cctgattctt tgaataaccc caataaggct cctgctgacc cttctgccgt
     4141 tctaggaaac cagcttgttg agtacgctgt gaacatcact cttggtatac ccggtcagcc
     4201 cttctcagtt cagattgaca caggctcgtc tgacttgtgg gtgaagagtg acggctcctc
     4261 cggtgcattc aacaagaagg cttcttctac ttttcaggag gacgttccca acggctttgc
     4321 aattgcccac ggagacaaaa cctctgccat tggagattgg gtcaaagata ccatcaatat
     4381 cggtggtgtt agtattgacc agtatagtat tgaccagtat gagttcgcca tggctactca
     4441 gacaaatact gacccggttt ttggtatcgg ctacccgagc aacgaggcgt cttatgatcg
     4501 cgtgcattcg cggccgcatt taaatcccca cttcagaagt tcctatactt tctagagaat
     4561 aggaacttca ctatagagtc gaataagggc gacaccccat aattagcccg ggcgaaaggc
     4621 ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcat
     4681 ggggagtccc cacactacca tcggcgctac ggcgtttcac ttctgagttc ggcatggggt
     4741 caggtgggac caccgcgcta ctgccgccag gcaaacaagg ggtgttatga gccatattca
     4801 ggtataaatg ggctcgcgat aatgttcaga attggttaat tggttgtaac actgacccct
     4861 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4921 taaatgcttc aataatattg aaaaaggaag aatatgagta ttcaacattt ccgtgtcgcc
     4981 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5041 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5101 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5161 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5221 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5281 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5341 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5401 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5461 gccataccaa acgacgagcg tgacaccacg atgcctgtag cgatggcaac aacgttgcgc
     5521 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5581 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5641 gctgataaat ccggagccgg tgagcgtggt tctcgcggta tcatcgcagc gctggggcca
     5701 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5761 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaaaagcag
     5821 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     5881 tacgcgcgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5941 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6001 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6061 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttagccc accacttcaa
     6121 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6181 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6241 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6301 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6361 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6421 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6481 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6541 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6601 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6661 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga
     6721 actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca
     6781 aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct
     6841 gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta
     6901 tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct
     6961 tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga
     7021 tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat
     7081 tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa
     7141 taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat
     7201 ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc
     7261 gcccttattc gactcactat agaagttcct attctctaga aagtatagga acttcacttc
     7321 attttattta aatttgcggc cgc
//
