LOCUS Exported 7357 bp ds-DNA linear SYN 20-DEC-2017 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pCfB4778 (pIntE-1-Ura-TPex20-TLip2) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7357) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 20, 2017 from SnapGene 4.1.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7357 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 14..513 /label=IntE-1_up terminator complement(527..839) /label=TPex20 misc_feature 840..868 /label=USER cloning site terminator 869..1805 /label=T lip2 misc_feature 1828..1861 /label=loxP promoter 1862..2863 /label=PrExp1 CDS 2864..3718 /label=Ura3 terminator 3719..3951 /label=TCyc misc_feature 3952..3985 /label=loxP misc_feature 4011..4510 /label=IntE-1_down misc_feature 4968..5828 /label=AmpR misc_feature complement(5929..6572) /label=pUC ORI ORIGIN 1 gaatgcgtgc gatttgatat ggtgtaacaa tgataaacca aggcccattg attgagacgg 61 agatattttc ggcgtgtcta gggtgtctac ttgtgcaccg gaacatggaa gatcgtgcgc 121 tgttcacttg caacagaagg ctaacgagag aaactgggcc aacccgtcta tgctcttcat 181 gctactcaat ctattcaaaa tacgccagtg ctccccttgc cttatacaca ggctcttcac 241 tcacatcttg accatcttgg atgtgggtac tgtagctcct gggaaccagt cgtgcgggag 301 catgagaacc acgtcgaaga agatggtgac ccaggaaagc aaaaacttgg gcacattctt 361 gtagataccc tgacctgtcg tggtcgaaga taccgtcgaa aaccagctgt cccattgaca 421 gaaaccctcc tcctgtatct agcagaacgc tggcaataga aaagccatta gtggactttc 481 gcgtgtagtt cgacgcagca gaggatagtg cttacgtgca acgcttacgc aactaacatg 541 aatgaatacg atatacatca aagactatga tacgcagtat tgcacactgt acgagtaagg 601 gcactagcca ctgcactcaa gtgaaaccgt tgcccgggta cgagtatgag tatgtacagt 661 atgtttagta ttgtacttgg acaatgcttg tatcgtacat tctcaagtgt caaacataaa 721 tatccgttgc tatatcctcg caccaccacg tagctcgcta tatccctgtg ttgaatccat 781 ccatcttgga ttgccaattg tgcacacaga accgggcact cacttcccca tccacacttc 841 aacggaatgc gtgcgatcgc gtgcattcct tctgttcgga atcaacctca aggttaacgg 901 ccacgatccc ctcgttgtta ctcttggtca gcccattgtc ggtaacgctg gctttgctaa 961 ctgggtcgat aaactcttct ttggccagga gaaccccgat gtctccaagg tgtccaaaga 1021 ccgaaagctc taccgaatca cccaccgagg agatatcgtc cctcaagtgc ccttctggga 1081 cggttaccag cactgctctg gtgaggtctt tattgactgg cccctgatcc accctcctct 1141 ctccaacgtt gtcatgtgcc agggccagag caataaacag tgctctgccg gtaacactct 1201 gctccagcag gtcaatgtga ttggaaacca tctgcagtac ttcgtcaccg agggtgtctg 1261 tggtatctaa gctatttatc actctttaca acttctacct caactatcta ctttaataaa 1321 tgaatatcgt ttattctcta tgattactgt atatgcgttc ctctaagaca aatcgaaacc 1381 agcatgcgat cgaatggcat acaaaagttt cttccgaagt tgatcaatgt cctgatagtc 1441 aggcagcttg agaagattga cacaggtgga ggccgtaggg aaccgatcaa cctgtctacc 1501 agcgttacga atggcaaatg acgggttcaa agccttgaat ccttgcaatg gtgccttgga 1561 tactgatgtc acaaacttaa gaagcagccg cttgtcctct tcctcgaaac tctcaaacac 1621 agtccagaag tcctttatag tttgatctgt atccagatag cctccgtaat tggtgtgtgt 1681 cttcaaatcc cagacgtcca cattggcatg tcctccactg ataagcattt gaagttcatc 1741 tgcgttgaac attgagaccc acgaagggtc aatgagctgg tatagaccgc ccaagaatgc 1801 atctgagaac agctgaagct tcgtacgata acttcgtata atgtatgcta tacgaagtta 1861 taaggagttt ggcgcccgtt ttttcgagcc ccacacgttt cggtgagtat gagcggcggc 1921 agattcgagc gtttccggtt tccgcggctg gacgagagcc catgatgggg gctcccacca 1981 ccagcaatca gggccctgat tacacaccca cctgtaatgt catgctgttc atcgtggtta 2041 atgctgctgt gtgctgtgtg tgtgtgttgt ttggcgctca ttgttgcgtt atgcagcgta 2101 caccacaata ttggaagctt attagccttt ctattttttc gtttgcaagg cttaacaaca 2161 ttgctgtgga gagggatggg gatatggagg ccgctggagg gagtcggaga ggcgttttgg 2221 agcggcttgg cctggcgccc agctcgcgaa acgcacctag gaccctttgg cacgccgaaa 2281 tgtgccactt ttcagtctag taacgcctta cctacgtcat tccatgcatg catgtttgcg 2341 ccttttttcc cttgcccttg atcgccacac agtacagtgc actgtacagt ggaggttttg 2401 ggggggtctt agatgggagc taaaagcggc ctagcggtac actagtggga ttgtatggag 2461 tggcatggag cctaggtgga gcctgacagg acgcacgacc ggctagcccg tgacagacga 2521 tgggtggctc ctgttgtcca ccgcgtacaa atgtttgggc caaagtcttg tcagccttgc 2581 ttgcgaacct aattcccaat tttgtcactt cgcaccccca ttgatcgagc cctaacccct 2641 gcccatcagg caatccaatt aagctcgcat tgtctgcctt gtttagtttg gctcctgccc 2701 gtttcggcgt ccacttgcac aaacacaaac aagcattata tataaggctc gtctctccct 2761 cccaaccaca ctcacttttt tgcccgtctt cccttgctaa cacaaaagtc aagaacacaa 2821 acaaccaccc caaccccctt acacacaaga catatctaca gcaatgccct cctacgaggc 2881 ccgagccaac gtccacaagt ccgccttcgc cgcccgagtc ctgaagctgg tcgccgccaa 2941 gaagaccaac ctgtgcgcct ccctggacgt caccaccacc aaggagctga tcgagctggc 3001 cgacaaggtc ggcccctacg tctgcatgat caagacccac atcgacatca tcgacgactt 3061 cacctacgcc ggcaccgtcc tgcccctgaa ggagctggcc ctgaagcacg gcttcttcct 3121 gttcgaggac cgaaagttcg ccgacatcgg caacaccgtc aagcaccagt accgatgcca 3181 ccgaatcgcc gagtggtccg acatcaccaa cgcccacggc gtccccggca ccggcatcat 3241 cgccggcctg cgagccggcg ccgaggagac cgtctccgag cagaagaagg aggacgtctc 3301 cgactacgag aactcccagt acaaggagtt cctggtcccc tcccccaacg agaagctggc 3361 ccgaggcctg ctgatgctgg ccgagctgtc ctgcaagggc tccctggcca ccggcgagta 3421 ctccaagcag accatcgagc tggcccgatc cgaccccgag ttcgtcgtcg gcttcatcgc 3481 ccagaaccga cccaagggcg actccgagga ctggctgatc ctgacccccg gcgtcggcct 3541 ggacgacaag ggcgacgccc tgggccagca gtaccgaacc gtcgaggacg tcatgtccac 3601 cggcaccgac atcatcatcg tcggccgagg cctgtacggc cagaaccgag accccatcga 3661 ggaggccaag cgataccaga aggccggctg ggaggcctac cagaagatca actgctagtc 3721 atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa 3781 aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta 3841 gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac 3901 gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc gataacttcg 3961 tataatgtat gctatacgaa gttatgtgat atcagatcca ctagtggcct ccgagcgtcg 4021 acaagcatac agccctcggg tggttgtgtc gaaatacaac agccagtcct ccacgatcca 4081 gctcagatag ccctgggtgt tgagatagag acaagtcgag cgagatgttc tcagtggact 4141 tgagtttgat gttgtgaaag atggttggat tgagagcaca actccataga acatagtagt 4201 ctcagtccat gagctaggag agaatctcca gtgctgatgt catttgaaga agaagaggaa 4261 gaagaagaca gatagatcaa ctcagcccct tccagttgcc gtacaatgac tcaaccaaat 4321 acgttcaagc ctgctttcaa gcctgcgtgc tctttctgtc tcattagttg aacgtagatc 4381 atctgagaga acttggcaga ccgtgcaaac acaagggcga gactgatagg acacttgatc 4441 tgatccaaaa tttgacagag gaaaactagt tttgaagtta ctgaacgttt cttctccaat 4501 tgcttctaac atcgcgtgca ttcgcggccg catttaaatc cccacttcag aagttcctat 4561 actttctaga gaataggaac ttcactatag agtcgaataa gggcgacacc ccataattag 4621 cccgggcgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggcagtt 4681 ccctactctc gcatggggag tccccacact accatcggcg ctacggcgtt tcacttctga 4741 gttcggcatg gggtcaggtg ggaccaccgc gctactgccg ccaggcaaac aaggggtgtt 4801 atgagccata ttcaggtata aatgggctcg cgataatgtt cagaattggt taattggttg 4861 taacactgac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 4921 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagaatatg agtattcaac 4981 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 5041 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 5101 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 5161 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 5221 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 5281 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 5341 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 5401 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 5461 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcgatgg 5521 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 5581 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 5641 ctggctggtt tattgctgat aaatccggag ccggtgagcg tggttctcgc ggtatcatcg 5701 cagcgctggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 5761 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 5821 attggtaaaa gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat 5881 cccttaacgt gagttacgcg cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc 5941 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 6001 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 6061 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 6121 gcccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 6181 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 6241 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 6301 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 6361 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 6421 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 6481 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 6541 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 6601 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 6661 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 6721 ggcgagagta gggaactgcc aggcatcaaa ctaagcagaa ggcccctgac ggatggcctt 6781 tttgcgtttc tacaaactct ttctgtgttg taaaacgacg gccagtctta agctcgggcc 6841 ccctgggcgg ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc 6901 ggcgggtttt tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaagggcg 6961 cctgtcactt tgcttgatat atgagaatta tttaacctta taaatgagaa aaaagcaacg 7021 cactttaaat aagatacgtt gctttttcga ttgatgaaca cctataatta aactattcat 7081 ctattattta tgattttttg tatatacaat atttctagtt tgttaaagag aattaagaaa 7141 ataaatctcg aaaataataa agggaaaatc agtttttgat atcaaaatta tacatgtcaa 7201 cgataataca aaatataata caaactataa gatgttatca gtatttatta tgcatttaga 7261 ataaattttg tgtcgccctt attcgactca ctatagaagt tcctattctc tagaaagtat 7321 aggaacttca cttcatttta tttaaatttg cggccgc //