LOCUS       Exported                7357 bp ds-DNA     linear   SYN 20-DEC-2017
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCfB4778 (pIntE-1-Ura-TPex20-TLip2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2017 from SnapGene 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    14..513
                     /label=IntE-1_up
     terminator      complement(527..839)
                     /label=TPex20
     misc_feature    840..868
                     /label=USER cloning site
     terminator      869..1805
                     /label=T lip2
     misc_feature    1828..1861
                     /label=loxP
     promoter        1862..2863
                     /label=PrExp1
     CDS             2864..3718
                     /label=Ura3
     terminator      3719..3951
                     /label=TCyc
     misc_feature    3952..3985
                     /label=loxP
     misc_feature    4011..4510
                     /label=IntE-1_down
     misc_feature    4968..5828
                     /label=AmpR
     misc_feature    complement(5929..6572)
                     /label=pUC ORI
ORIGIN
        1 gaatgcgtgc gatttgatat ggtgtaacaa tgataaacca aggcccattg attgagacgg
       61 agatattttc ggcgtgtcta gggtgtctac ttgtgcaccg gaacatggaa gatcgtgcgc
      121 tgttcacttg caacagaagg ctaacgagag aaactgggcc aacccgtcta tgctcttcat
      181 gctactcaat ctattcaaaa tacgccagtg ctccccttgc cttatacaca ggctcttcac
      241 tcacatcttg accatcttgg atgtgggtac tgtagctcct gggaaccagt cgtgcgggag
      301 catgagaacc acgtcgaaga agatggtgac ccaggaaagc aaaaacttgg gcacattctt
      361 gtagataccc tgacctgtcg tggtcgaaga taccgtcgaa aaccagctgt cccattgaca
      421 gaaaccctcc tcctgtatct agcagaacgc tggcaataga aaagccatta gtggactttc
      481 gcgtgtagtt cgacgcagca gaggatagtg cttacgtgca acgcttacgc aactaacatg
      541 aatgaatacg atatacatca aagactatga tacgcagtat tgcacactgt acgagtaagg
      601 gcactagcca ctgcactcaa gtgaaaccgt tgcccgggta cgagtatgag tatgtacagt
      661 atgtttagta ttgtacttgg acaatgcttg tatcgtacat tctcaagtgt caaacataaa
      721 tatccgttgc tatatcctcg caccaccacg tagctcgcta tatccctgtg ttgaatccat
      781 ccatcttgga ttgccaattg tgcacacaga accgggcact cacttcccca tccacacttc
      841 aacggaatgc gtgcgatcgc gtgcattcct tctgttcgga atcaacctca aggttaacgg
      901 ccacgatccc ctcgttgtta ctcttggtca gcccattgtc ggtaacgctg gctttgctaa
      961 ctgggtcgat aaactcttct ttggccagga gaaccccgat gtctccaagg tgtccaaaga
     1021 ccgaaagctc taccgaatca cccaccgagg agatatcgtc cctcaagtgc ccttctggga
     1081 cggttaccag cactgctctg gtgaggtctt tattgactgg cccctgatcc accctcctct
     1141 ctccaacgtt gtcatgtgcc agggccagag caataaacag tgctctgccg gtaacactct
     1201 gctccagcag gtcaatgtga ttggaaacca tctgcagtac ttcgtcaccg agggtgtctg
     1261 tggtatctaa gctatttatc actctttaca acttctacct caactatcta ctttaataaa
     1321 tgaatatcgt ttattctcta tgattactgt atatgcgttc ctctaagaca aatcgaaacc
     1381 agcatgcgat cgaatggcat acaaaagttt cttccgaagt tgatcaatgt cctgatagtc
     1441 aggcagcttg agaagattga cacaggtgga ggccgtaggg aaccgatcaa cctgtctacc
     1501 agcgttacga atggcaaatg acgggttcaa agccttgaat ccttgcaatg gtgccttgga
     1561 tactgatgtc acaaacttaa gaagcagccg cttgtcctct tcctcgaaac tctcaaacac
     1621 agtccagaag tcctttatag tttgatctgt atccagatag cctccgtaat tggtgtgtgt
     1681 cttcaaatcc cagacgtcca cattggcatg tcctccactg ataagcattt gaagttcatc
     1741 tgcgttgaac attgagaccc acgaagggtc aatgagctgg tatagaccgc ccaagaatgc
     1801 atctgagaac agctgaagct tcgtacgata acttcgtata atgtatgcta tacgaagtta
     1861 taaggagttt ggcgcccgtt ttttcgagcc ccacacgttt cggtgagtat gagcggcggc
     1921 agattcgagc gtttccggtt tccgcggctg gacgagagcc catgatgggg gctcccacca
     1981 ccagcaatca gggccctgat tacacaccca cctgtaatgt catgctgttc atcgtggtta
     2041 atgctgctgt gtgctgtgtg tgtgtgttgt ttggcgctca ttgttgcgtt atgcagcgta
     2101 caccacaata ttggaagctt attagccttt ctattttttc gtttgcaagg cttaacaaca
     2161 ttgctgtgga gagggatggg gatatggagg ccgctggagg gagtcggaga ggcgttttgg
     2221 agcggcttgg cctggcgccc agctcgcgaa acgcacctag gaccctttgg cacgccgaaa
     2281 tgtgccactt ttcagtctag taacgcctta cctacgtcat tccatgcatg catgtttgcg
     2341 ccttttttcc cttgcccttg atcgccacac agtacagtgc actgtacagt ggaggttttg
     2401 ggggggtctt agatgggagc taaaagcggc ctagcggtac actagtggga ttgtatggag
     2461 tggcatggag cctaggtgga gcctgacagg acgcacgacc ggctagcccg tgacagacga
     2521 tgggtggctc ctgttgtcca ccgcgtacaa atgtttgggc caaagtcttg tcagccttgc
     2581 ttgcgaacct aattcccaat tttgtcactt cgcaccccca ttgatcgagc cctaacccct
     2641 gcccatcagg caatccaatt aagctcgcat tgtctgcctt gtttagtttg gctcctgccc
     2701 gtttcggcgt ccacttgcac aaacacaaac aagcattata tataaggctc gtctctccct
     2761 cccaaccaca ctcacttttt tgcccgtctt cccttgctaa cacaaaagtc aagaacacaa
     2821 acaaccaccc caaccccctt acacacaaga catatctaca gcaatgccct cctacgaggc
     2881 ccgagccaac gtccacaagt ccgccttcgc cgcccgagtc ctgaagctgg tcgccgccaa
     2941 gaagaccaac ctgtgcgcct ccctggacgt caccaccacc aaggagctga tcgagctggc
     3001 cgacaaggtc ggcccctacg tctgcatgat caagacccac atcgacatca tcgacgactt
     3061 cacctacgcc ggcaccgtcc tgcccctgaa ggagctggcc ctgaagcacg gcttcttcct
     3121 gttcgaggac cgaaagttcg ccgacatcgg caacaccgtc aagcaccagt accgatgcca
     3181 ccgaatcgcc gagtggtccg acatcaccaa cgcccacggc gtccccggca ccggcatcat
     3241 cgccggcctg cgagccggcg ccgaggagac cgtctccgag cagaagaagg aggacgtctc
     3301 cgactacgag aactcccagt acaaggagtt cctggtcccc tcccccaacg agaagctggc
     3361 ccgaggcctg ctgatgctgg ccgagctgtc ctgcaagggc tccctggcca ccggcgagta
     3421 ctccaagcag accatcgagc tggcccgatc cgaccccgag ttcgtcgtcg gcttcatcgc
     3481 ccagaaccga cccaagggcg actccgagga ctggctgatc ctgacccccg gcgtcggcct
     3541 ggacgacaag ggcgacgccc tgggccagca gtaccgaacc gtcgaggacg tcatgtccac
     3601 cggcaccgac atcatcatcg tcggccgagg cctgtacggc cagaaccgag accccatcga
     3661 ggaggccaag cgataccaga aggccggctg ggaggcctac cagaagatca actgctagtc
     3721 atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa
     3781 aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta
     3841 gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac
     3901 gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc gataacttcg
     3961 tataatgtat gctatacgaa gttatgtgat atcagatcca ctagtggcct ccgagcgtcg
     4021 acaagcatac agccctcggg tggttgtgtc gaaatacaac agccagtcct ccacgatcca
     4081 gctcagatag ccctgggtgt tgagatagag acaagtcgag cgagatgttc tcagtggact
     4141 tgagtttgat gttgtgaaag atggttggat tgagagcaca actccataga acatagtagt
     4201 ctcagtccat gagctaggag agaatctcca gtgctgatgt catttgaaga agaagaggaa
     4261 gaagaagaca gatagatcaa ctcagcccct tccagttgcc gtacaatgac tcaaccaaat
     4321 acgttcaagc ctgctttcaa gcctgcgtgc tctttctgtc tcattagttg aacgtagatc
     4381 atctgagaga acttggcaga ccgtgcaaac acaagggcga gactgatagg acacttgatc
     4441 tgatccaaaa tttgacagag gaaaactagt tttgaagtta ctgaacgttt cttctccaat
     4501 tgcttctaac atcgcgtgca ttcgcggccg catttaaatc cccacttcag aagttcctat
     4561 actttctaga gaataggaac ttcactatag agtcgaataa gggcgacacc ccataattag
     4621 cccgggcgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggcagtt
     4681 ccctactctc gcatggggag tccccacact accatcggcg ctacggcgtt tcacttctga
     4741 gttcggcatg gggtcaggtg ggaccaccgc gctactgccg ccaggcaaac aaggggtgtt
     4801 atgagccata ttcaggtata aatgggctcg cgataatgtt cagaattggt taattggttg
     4861 taacactgac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4921 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagaatatg agtattcaac
     4981 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5041 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5101 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5161 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5221 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5281 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5341 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5401 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5461 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcgatgg
     5521 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5581 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5641 ctggctggtt tattgctgat aaatccggag ccggtgagcg tggttctcgc ggtatcatcg
     5701 cagcgctggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5761 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5821 attggtaaaa gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     5881 cccttaacgt gagttacgcg cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     5941 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6001 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6061 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6121 gcccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6181 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6241 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6301 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6361 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6421 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6481 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6541 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6601 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6661 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6721 ggcgagagta gggaactgcc aggcatcaaa ctaagcagaa ggcccctgac ggatggcctt
     6781 tttgcgtttc tacaaactct ttctgtgttg taaaacgacg gccagtctta agctcgggcc
     6841 ccctgggcgg ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc
     6901 ggcgggtttt tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaagggcg
     6961 cctgtcactt tgcttgatat atgagaatta tttaacctta taaatgagaa aaaagcaacg
     7021 cactttaaat aagatacgtt gctttttcga ttgatgaaca cctataatta aactattcat
     7081 ctattattta tgattttttg tatatacaat atttctagtt tgttaaagag aattaagaaa
     7141 ataaatctcg aaaataataa agggaaaatc agtttttgat atcaaaatta tacatgtcaa
     7201 cgataataca aaatataata caaactataa gatgttatca gtatttatta tgcatttaga
     7261 ataaattttg tgtcgccctt attcgactca ctatagaagt tcctattctc tagaaagtat
     7321 aggaacttca cttcatttta tttaaatttg cggccgc
//
