LOCUS       Exported                7586 bp ds-DNA     circular SYN 20-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCfB4132 (pIntB-HpHSyn-TPex20-TLip2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2017 from SnapGene 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    14..513
                     /label=upstream IntB
     terminator      complement(574..886)
                     /label=TPex20
     misc_feature    887..915
                     /label=USER cloning site
     terminator      916..1852
                     /label=T lip2
     misc_feature    1862..1895
                     /label=loxP
     promoter        1896..2897
                     /label=PrExp1
     CDS             2898..3926
                     /label=HphSyn
     terminator      3927..4159
                     /label=Tcyc1
     misc_feature    4160..4193
                     /label=loxP
     misc_feature    4254..4739
                     /label=IntB down
     misc_feature    5197..6057
                     /label=AmpR
     misc_feature    complement(6158..6801)
                     /label=pUC ORI
ORIGIN
        1 gaatgcgtgc gatcccacag ttctcactca gatcatggag actctaacct tgagacatca
       61 attatcagct ctcgaggata atgttagtgc agttccagga ctcattgtgc aactgtcacc
      121 acggcatttt gggtctgttc ttttgaagta cagaaatatc ctcattgttg gtatactttg
      181 ggactttttc ttgttacaga agaataaaaa aacctcgact gatgtactaa ttacatggtt
      241 aacatcccca aggtcaaagt acagatattg taccgacttc tgaaatttgt gggatccaca
      301 cacggacctc tgcgatgata caatatcatg gttccatggt ctcttgaatc acaccactca
      361 ataataaaca ccagctcttt caatccaact tagctttctt ccacttgaac ataggccatc
      421 cccccttctt tctattttac ataaatagca agatccttac ccctacatgt ctcataacac
      481 aatctcaact tgacttccca taagaagttc actcagtcat gaaagtctta gtactggacg
      541 tgcaacgctt cagatgtgac catatactta ggcacgcaac taacatgaat gaatacgata
      601 tacatcaaag actatgatac gcagtattgc acactgtacg agtaagggca ctagccactg
      661 cactcaagtg aaaccgttgc ccgggtacga gtatgagtat gtacagtatg tttagtattg
      721 tacttggaca atgcttgtat cgtacattct caagtgtcaa acataaatat ccgttgctat
      781 atcctcgcac caccacgtag ctcgctatat ccctgtgttg aatccatcca tcttggattg
      841 ccaattgtgc acacagaacc gggcactcac ttccccatcc acacttcaac ggaatgcgtg
      901 cgatcgcgtg cattccttct gttcggaatc aacctcaagg ttaacggcca cgatcccctc
      961 gttgttactc ttggtcagcc cattgtcggt aacgctggct ttgctaactg ggtcgataaa
     1021 ctcttctttg gccaggagaa ccccgatgtc tccaaggtgt ccaaagaccg aaagctctac
     1081 cgaatcaccc accgaggaga tatcgtccct caagtgccct tctgggacgg ttaccagcac
     1141 tgctctggtg aggtctttat tgactggccc ctgatccacc ctcctctctc caacgttgtc
     1201 atgtgccagg gccagagcaa taaacagtgc tctgccggta acactctgct ccagcaggtc
     1261 aatgtgattg gaaaccatct gcagtacttc gtcaccgagg gtgtctgtgg tatctaagct
     1321 atttatcact ctttacaact tctacctcaa ctatctactt taataaatga atatcgttta
     1381 ttctctatga ttactgtata tgcgttcctc taagacaaat cgaaaccagc atgcgatcga
     1441 atggcataca aaagtttctt ccgaagttga tcaatgtcct gatagtcagg cagcttgaga
     1501 agattgacac aggtggaggc cgtagggaac cgatcaacct gtctaccagc gttacgaatg
     1561 gcaaatgacg ggttcaaagc cttgaatcct tgcaatggtg ccttggatac tgatgtcaca
     1621 aacttaagaa gcagccgctt gtcctcttcc tcgaaactct caaacacagt ccagaagtcc
     1681 tttatagttt gatctgtatc cagatagcct ccgtaattgg tgtgtgtctt caaatcccag
     1741 acgtccacat tggcatgtcc tccactgata agcatttgaa gttcatctgc gttgaacatt
     1801 gagacccacg aagggtcaat gagctggtat agaccgccca agaatgcatc tgatctgtca
     1861 tataacttcg tataatgtat gctatacgaa gttataagga gtttggcgcc cgttttttcg
     1921 agccccacac gtttcggtga gtatgagcgg cggcagattc gagcgtttcc ggtttccgcg
     1981 gctggacgag agcccatgat gggggctccc accaccagca atcagggccc tgattacaca
     2041 cccacctgta atgtcatgct gttcatcgtg gttaatgctg ctgtgtgctg tgtgtgtgtg
     2101 ttgtttggcg ctcattgttg cgttatgcag cgtacaccac aatattggaa gcttattagc
     2161 ctttctattt tttcgtttgc aaggcttaac aacattgctg tggagaggga tggggatatg
     2221 gaggccgctg gagggagtcg gagaggcgtt ttggagcggc ttggcctggc gcccagctcg
     2281 cgaaacgcac ctaggaccct ttggcacgcc gaaatgtgcc acttttcagt ctagtaacgc
     2341 cttacctacg tcattccatg catgcatgtt tgcgcctttt ttcccttgcc cttgatcgcc
     2401 acacagtaca gtgcactgta cagtggaggt tttggggggg tcttagatgg gagctaaaag
     2461 cggcctagcg gtacactagt gggattgtat ggagtggcat ggagcctagg tggagcctga
     2521 caggacgcac gaccggctag cccgtgacag acgatgggtg gctcctgttg tccaccgcgt
     2581 acaaatgttt gggccaaagt cttgtcagcc ttgcttgcga acctaattcc caattttgtc
     2641 acttcgcacc cccattgatc gagccctaac ccctgcccat caggcaatcc aattaagctc
     2701 gcattgtctg ccttgtttag tttggctcct gcccgtttcg gcgtccactt gcacaaacac
     2761 aaacaagcat tatatataag gctcgtctct ccctcccaac cacactcact tttttgcccg
     2821 tcttcccttg ctaacacaaa agtcaagaac acaaacaacc accccaaccc ccttacacac
     2881 aagacatatc tacagcaatg ggtaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     2941 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     3001 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     3061 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     3121 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     3181 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     3241 cggtcgcgga ggcaatggat gccattgctg cggccgatct tagccagacg agcgggttcg
     3301 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     3361 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     3421 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     3481 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     3541 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     3601 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     3661 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     3721 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     3781 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     3841 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     3901 gcactcgtcc gagggcaaag gaataatcat gtaattagtt atgtcacgct tacattcacg
     3961 ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
     4021 ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt
     4081 tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
     4141 gaaggttttg ggacgctcga taacttcgta taatgtatgc tatacgaagt tatcagcatc
     4201 gtaatagcct ccaagagatt gatcatcact ctgaatgtac aagcaaccca agtacctgct
     4261 cctgcaccta agttcgtcaa aatcggtttt actcagagaa tcaacaaccc taccaactgt
     4321 acatactgct aaccctgatt ctttgaataa ccccaataag gctcctgctg acccttctgc
     4381 cgttctagga aaccagcttg ttgagtacgc tgtgaacatc actcttggta tacccggtca
     4441 gcccttctca gttcagattg acacaggctc gtctgacttg tgggtgaaga gtgacggctc
     4501 ctccggtgca ttcaacaaga aggcttcttc tacttttcag gaggacgttc ccaacggctt
     4561 tgcaattgcc cacggagaca aaacctctgc cattggagat tgggtcaaag ataccatcaa
     4621 tatcggtggt gttagtattg accagtatag tattgaccag tatgagttcg ccatggctac
     4681 tcagacaaat actgacccgg tttttggtat cggctacccg agcaacgagg cgtcttatga
     4741 tcgcgtgcat tcgcggccgc atttaaatcc ccacttcaga agttcctata ctttctagag
     4801 aataggaact tcactataga gtcgaataag ggcgacaccc cataattagc ccgggcgaaa
     4861 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg
     4921 catggggagt ccccacacta ccatcggcgc tacggcgttt cacttctgag ttcggcatgg
     4981 ggtcaggtgg gaccaccgcg ctactgccgc caggcaaaca aggggtgtta tgagccatat
     5041 tcaggtataa atgggctcgc gataatgttc agaattggtt aattggttgt aacactgacc
     5101 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5161 tgataaatgc ttcaataata ttgaaaaagg aagaatatga gtattcaaca tttccgtgtc
     5221 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5281 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5341 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5401 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5461 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5521 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5581 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5641 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5701 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcgatggc aacaacgttg
     5761 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5821 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5881 attgctgata aatccggagc cggtgagcgt ggttctcgcg gtatcatcgc agcgctgggg
     5941 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6001 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaaaag
     6061 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     6121 agttacgcgc gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6181 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6241 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6301 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag cccaccactt
     6361 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6421 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6481 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6541 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6601 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6661 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6721 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6781 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6841 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     6901 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag gcgagagtag
     6961 ggaactgcca ggcatcaaac taagcagaag gcccctgacg gatggccttt ttgcgtttct
     7021 acaaactctt tctgtgttgt aaaacgacgg ccagtcttaa gctcgggccc cctgggcggt
     7081 tctgataacg agtaatcgtt aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt
     7141 ttatgggggg agtttaggga aagagcattt gtcagaatat ttaagggcgc ctgtcacttt
     7201 gcttgatata tgagaattat ttaaccttat aaatgagaaa aaagcaacgc actttaaata
     7261 agatacgttg ctttttcgat tgatgaacac ctataattaa actattcatc tattatttat
     7321 gattttttgt atatacaata tttctagttt gttaaagaga attaagaaaa taaatctcga
     7381 aaataataaa gggaaaatca gtttttgata tcaaaattat acatgtcaac gataatacaa
     7441 aatataatac aaactataag atgttatcag tatttattat gcatttagaa taaattttgt
     7501 gtcgccctta ttcgactcac tatagaagtt cctattctct agaaagtata ggaacttcac
     7561 ttcattttat ttaaatttgc ggccgc
//
