LOCUS       Exported                7722 bp ds-DNA     circular SYN 20-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCfB5935 (pIntA-1-HphMx-TPex20-TLip2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2017 from SnapGene 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7722
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    14..504
                     /label=IntA-1 UP
     terminator      complement(518..830)
                     /label=TPex20
     misc_feature    831..859
                     /label=USER cloning site
     terminator      860..1796
                     /label=T lip2
     misc_feature    1819..1852
                     /label=loxP
     promoter        1853..2854
                     /label=PrExp1
     CDS             2855..3883
                     /label=HphSyn
     terminator      3884..4296
                     /label=TTef
     misc_feature    4297..4330
                     /label=loxP
     misc_feature    4356..4875
                     /label=IntA_1 DW
     misc_feature    5333..6193
                     /label=AmpR
     misc_feature    complement(6294..6937)
                     /label=pUC ORI
ORIGIN
        1 gaatgcgtgc gatttattat cccaaaaata ccccactctc cattatttcc gactgtgcag
       61 attcccatat caacccaatc tccatcaaaa caccgcagcc aaactctggc aaaagtgcaa
      121 catgcgatac ggacacggtg ggcggtgggc atcgatcaac tggccgtagg tgcatctggg
      181 ctggaattac cccacagtgc agtgctaagc agtggtgatg gaggatattg ggggatggaa
      241 gatattgggg gatggaggga gaggactaga cctctcagta acagtgcaag cagtacatgc
      301 aatacaagta acacccacca acacacggac cagacgagac caaccaactg cacagcccta
      361 ccaggaaacg gcagagcttg tggacacaac agactgcgac aagggacccg ctttgccagt
      421 gtgatgcagg aaccatactc ttttcctttt ttcggacagc cggcacacga gcttgacgat
      481 caaaccatat cggccgagtg cgacacgtgc aacgcttacg caactaacat gaatgaatac
      541 gatatacatc aaagactatg atacgcagta ttgcacactg tacgagtaag ggcactagcc
      601 actgcactca agtgaaaccg ttgcccgggt acgagtatga gtatgtacag tatgtttagt
      661 attgtacttg gacaatgctt gtatcgtaca ttctcaagtg tcaaacataa atatccgttg
      721 ctatatcctc gcaccaccac gtagctcgct atatccctgt gttgaatcca tccatcttgg
      781 attgccaatt gtgcacacag aaccgggcac tcacttcccc atccacactt caacggaatg
      841 cgtgcgatcg cgtgcattcc ttctgttcgg aatcaacctc aaggttaacg gccacgatcc
      901 cctcgttgtt actcttggtc agcccattgt cggtaacgct ggctttgcta actgggtcga
      961 taaactcttc tttggccagg agaaccccga tgtctccaag gtgtccaaag accgaaagct
     1021 ctaccgaatc acccaccgag gagatatcgt ccctcaagtg cccttctggg acggttacca
     1081 gcactgctct ggtgaggtct ttattgactg gcccctgatc caccctcctc tctccaacgt
     1141 tgtcatgtgc cagggccaga gcaataaaca gtgctctgcc ggtaacactc tgctccagca
     1201 ggtcaatgtg attggaaacc atctgcagta cttcgtcacc gagggtgtct gtggtatcta
     1261 agctatttat cactctttac aacttctacc tcaactatct actttaataa atgaatatcg
     1321 tttattctct atgattactg tatatgcgtt cctctaagac aaatcgaaac cagcatgcga
     1381 tcgaatggca tacaaaagtt tcttccgaag ttgatcaatg tcctgatagt caggcagctt
     1441 gagaagattg acacaggtgg aggccgtagg gaaccgatca acctgtctac cagcgttacg
     1501 aatggcaaat gacgggttca aagccttgaa tccttgcaat ggtgccttgg atactgatgt
     1561 cacaaactta agaagcagcc gcttgtcctc ttcctcgaaa ctctcaaaca cagtccagaa
     1621 gtcctttata gtttgatctg tatccagata gcctccgtaa ttggtgtgtg tcttcaaatc
     1681 ccagacgtcc acattggcat gtcctccact gataagcatt tgaagttcat ctgcgttgaa
     1741 cattgagacc cacgaagggt caatgagctg gtatagaccg cccaagaatg catctgagaa
     1801 cagctgaagc ttcgtacgat aacttcgtat aatgtatgct atacgaagtt ataaggagtt
     1861 tggcgcccgt tttttcgagc cccacacgtt tcggtgagta tgagcggcgg cagattcgag
     1921 cgtttccggt ttccgcggct ggacgagagc ccatgatggg ggctcccacc accagcaatc
     1981 agggccctga ttacacaccc acctgtaatg tcatgctgtt catcgtggtt aatgctgctg
     2041 tgtgctgtgt gtgtgtgttg tttggcgctc attgttgcgt tatgcagcgt acaccacaat
     2101 attggaagct tattagcctt tctatttttt cgtttgcaag gcttaacaac attgctgtgg
     2161 agagggatgg ggatatggag gccgctggag ggagtcggag aggcgttttg gagcggcttg
     2221 gcctggcgcc cagctcgcga aacgcaccta ggaccctttg gcacgccgaa atgtgccact
     2281 tttcagtcta gtaacgcctt acctacgtca ttccatgcat gcatgtttgc gccttttttc
     2341 ccttgccctt gatcgccaca cagtacagtg cactgtacag tggaggtttt gggggggtct
     2401 tagatgggag ctaaaagcgg cctagcggta cactagtggg attgtatgga gtggcatgga
     2461 gcctaggtgg agcctgacag gacgcacgac cggctagccc gtgacagacg atgggtggct
     2521 cctgttgtcc accgcgtaca aatgtttggg ccaaagtctt gtcagccttg cttgcgaacc
     2581 taattcccaa ttttgtcact tcgcaccccc attgatcgag ccctaacccc tgcccatcag
     2641 gcaatccaat taagctcgca ttgtctgcct tgtttagttt ggctcctgcc cgtttcggcg
     2701 tccacttgca caaacacaaa caagcattat atataaggct cgtctctccc tcccaaccac
     2761 actcactttt ttgcccgtct tcccttgcta acacaaaagt caagaacaca aacaaccacc
     2821 ccaaccccct tacacacaag acatatctac agcaatgggt aaaaagcctg aactcaccgc
     2881 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     2941 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     3001 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     3061 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
     3121 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     3181 gcccgctgtt ctgcagccgg tcgcggaggc aatggatgcc attgctgcgg ccgatcttag
     3241 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     3301 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     3361 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     3421 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     3481 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     3541 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     3601 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
     3661 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     3721 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     3781 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     3841 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa taaccccacg ttgccggtct
     3901 tgcctcctac tacctgtcca tcaatgacga ggttctcacc cctgcccagg tcgaggctct
     3961 tattactgag tccaacaccg gtgttcttcc caccaccaac ctcaagggct ctcccaacgc
     4021 tgttgcctac aacggtgttg gcatttaggc aattaacaga tagtttgccg gtgataattc
     4081 tcttaacctc ccacactcct ttgacataac gatttatgta acgaaactga aatttgacca
     4141 gatattgttg taaatagaaa atctggcttg taggtggcaa aatgcggcgt ctttgttcat
     4201 caattccctc tgtgactact cgtcatccct ttatgttcga ctgtcgtatt tcttattttc
     4261 catacatatg caagtgagat gcccgtgtcc gaattcataa cttcgtataa tgtatgctat
     4321 acgaagttat gtgatatcag atccactagt ggcctggggc tggcgtgtga aggagcatgt
     4381 gcaaagggcc gcgcagactt gtggagtgct gccgtacgag tatcggtgta cgacaaggtg
     4441 gtttgtacaa ggtggtttgt accgtcggac atgatcatct gtaccttggc gtactgtact
     4501 tgtacaacca cttggtgatc agatataacc taacagacta atcgaccgag gcattcatac
     4561 tcacccgaac cagcctaaac gagataaccc ccacagttgg ccgttaggtt agagtcttgt
     4621 tcggttcttg ggttcttcag cttttgtttt tccgctgccc gtcattgtct tctctttctt
     4681 tgtgctccaa acctttcgcc agggcctgga cggatgccgt attcaaacag gttcatgtat
     4741 ggaaatgact ttggatacgg ccttggatac gaccttggat acgaccttgg atacgacctt
     4801 ggatacgacg tcggctgcga ttacgtgcag agggagagct acatgttcca tgtgcgatgc
     4861 cgtgtgagag gtctgatcgc gtgcattcgc ggccgcattt aaatccccac ttcagaagtt
     4921 cctatacttt ctagagaata ggaacttcac tatagagtcg aataagggcg acaccccata
     4981 attagcccgg gcgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg
     5041 cagttcccta ctctcgcatg gggagtcccc acactaccat cggcgctacg gcgtttcact
     5101 tctgagttcg gcatggggtc aggtgggacc accgcgctac tgccgccagg caaacaaggg
     5161 gtgttatgag ccatattcag gtataaatgg gctcgcgata atgttcagaa ttggttaatt
     5221 ggttgtaaca ctgaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5281 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga atatgagtat
     5341 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5401 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5461 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5521 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     5581 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5641 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5701 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5761 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5821 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5881 gatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5941 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6001 tccggctggc tggtttattg ctgataaatc cggagccggt gagcgtggtt ctcgcggtat
     6061 catcgcagcg ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6121 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6181 taagcattgg taaaagcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     6241 aaaatccctt aacgtgagtt acgcgcgcgt cgttccactg agcgtcagac cccgtagaaa
     6301 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6361 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6421 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6481 agttagccca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6541 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6601 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6661 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6721 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6781 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6841 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6901 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6961 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7021 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7081 cggaaggcga gagtagggaa ctgccaggca tcaaactaag cagaaggccc ctgacggatg
     7141 gcctttttgc gtttctacaa actctttctg tgttgtaaaa cgacggccag tcttaagctc
     7201 gggccccctg ggcggttctg ataacgagta atcgttaatc cgcaaataac gtaaaaaccc
     7261 gcttcggcgg gtttttttat ggggggagtt tagggaaaga gcatttgtca gaatatttaa
     7321 gggcgcctgt cactttgctt gatatatgag aattatttaa ccttataaat gagaaaaaag
     7381 caacgcactt taaataagat acgttgcttt ttcgattgat gaacacctat aattaaacta
     7441 ttcatctatt atttatgatt ttttgtatat acaatatttc tagtttgtta aagagaatta
     7501 agaaaataaa tctcgaaaat aataaaggga aaatcagttt ttgatatcaa aattatacat
     7561 gtcaacgata atacaaaata taatacaaac tataagatgt tatcagtatt tattatgcat
     7621 ttagaataaa ttttgtgtcg cccttattcg actcactata gaagttccta ttctctagaa
     7681 agtataggaa cttcacttca ttttatttaa atttgcggcc gc
//
