LOCUS Exported 7711 bp ds-DNA circular SYN 20-DEC-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCfB6574 (pIntE_1-HphMx-TPex20-TLip2) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7711) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 20, 2017 from SnapGene 4.1.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7711 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 14..513 /label=IntE-1_up terminator complement(527..839) /label=TPex20 misc_feature 840..868 /label=USER cloning site terminator 869..1805 /label=T lip2 misc_feature 1828..1861 /label=loxP promoter 1862..2863 /label=PrExp1 CDS 2864..3892 /label=HphSyn terminator 3893..4305 /label=Ttef /label=Tcyc1 misc_feature 4306..4339 /label=loxP misc_feature 4365..4864 /label=IntE-1_down misc_feature 5322..6182 /label=AmpR misc_feature complement(6283..6926) /label=pUC ORI ORIGIN 1 gaatgcgtgc gatttgatat ggtgtaacaa tgataaacca aggcccattg attgagacgg 61 agatattttc ggcgtgtcta gggtgtctac ttgtgcaccg gaacatggaa gatcgtgcgc 121 tgttcacttg caacagaagg ctaacgagag aaactgggcc aacccgtcta tgctcttcat 181 gctactcaat ctattcaaaa tacgccagtg ctccccttgc cttatacaca ggctcttcac 241 tcacatcttg accatcttgg atgtgggtac tgtagctcct gggaaccagt cgtgcgggag 301 catgagaacc acgtcgaaga agatggtgac ccaggaaagc aaaaacttgg gcacattctt 361 gtagataccc tgacctgtcg tggtcgaaga taccgtcgaa aaccagctgt cccattgaca 421 gaaaccctcc tcctgtatct agcagaacgc tggcaataga aaagccatta gtggactttc 481 gcgtgtagtt cgacgcagca gaggatagtg cttacgtgca acgcttacgc aactaacatg 541 aatgaatacg atatacatca aagactatga tacgcagtat tgcacactgt acgagtaagg 601 gcactagcca ctgcactcaa gtgaaaccgt tgcccgggta cgagtatgag tatgtacagt 661 atgtttagta ttgtacttgg acaatgcttg tatcgtacat tctcaagtgt caaacataaa 721 tatccgttgc tatatcctcg caccaccacg tagctcgcta tatccctgtg ttgaatccat 781 ccatcttgga ttgccaattg tgcacacaga accgggcact cacttcccca tccacacttc 841 aacggaatgc gtgcgatcgc gtgcattcct tctgttcgga atcaacctca aggttaacgg 901 ccacgatccc ctcgttgtta ctcttggtca gcccattgtc ggtaacgctg gctttgctaa 961 ctgggtcgat aaactcttct ttggccagga gaaccccgat gtctccaagg tgtccaaaga 1021 ccgaaagctc taccgaatca cccaccgagg agatatcgtc cctcaagtgc ccttctggga 1081 cggttaccag cactgctctg gtgaggtctt tattgactgg cccctgatcc accctcctct 1141 ctccaacgtt gtcatgtgcc agggccagag caataaacag tgctctgccg gtaacactct 1201 gctccagcag gtcaatgtga ttggaaacca tctgcagtac ttcgtcaccg agggtgtctg 1261 tggtatctaa gctatttatc actctttaca acttctacct caactatcta ctttaataaa 1321 tgaatatcgt ttattctcta tgattactgt atatgcgttc ctctaagaca aatcgaaacc 1381 agcatgcgat cgaatggcat acaaaagttt cttccgaagt tgatcaatgt cctgatagtc 1441 aggcagcttg agaagattga cacaggtgga ggccgtaggg aaccgatcaa cctgtctacc 1501 agcgttacga atggcaaatg acgggttcaa agccttgaat ccttgcaatg gtgccttgga 1561 tactgatgtc acaaacttaa gaagcagccg cttgtcctct tcctcgaaac tctcaaacac 1621 agtccagaag tcctttatag tttgatctgt atccagatag cctccgtaat tggtgtgtgt 1681 cttcaaatcc cagacgtcca cattggcatg tcctccactg ataagcattt gaagttcatc 1741 tgcgttgaac attgagaccc acgaagggtc aatgagctgg tatagaccgc ccaagaatgc 1801 atctgagaac agctgaagct tcgtacgata acttcgtata atgtatgcta tacgaagtta 1861 taaggagttt ggcgcccgtt ttttcgagcc ccacacgttt cggtgagtat gagcggcggc 1921 agattcgagc gtttccggtt tccgcggctg gacgagagcc catgatgggg gctcccacca 1981 ccagcaatca gggccctgat tacacaccca cctgtaatgt catgctgttc atcgtggtta 2041 atgctgctgt gtgctgtgtg tgtgtgttgt ttggcgctca ttgttgcgtt atgcagcgta 2101 caccacaata ttggaagctt attagccttt ctattttttc gtttgcaagg cttaacaaca 2161 ttgctgtgga gagggatggg gatatggagg ccgctggagg gagtcggaga ggcgttttgg 2221 agcggcttgg cctggcgccc agctcgcgaa acgcacctag gaccctttgg cacgccgaaa 2281 tgtgccactt ttcagtctag taacgcctta cctacgtcat tccatgcatg catgtttgcg 2341 ccttttttcc cttgcccttg atcgccacac agtacagtgc actgtacagt ggaggttttg 2401 ggggggtctt agatgggagc taaaagcggc ctagcggtac actagtggga ttgtatggag 2461 tggcatggag cctaggtgga gcctgacagg acgcacgacc ggctagcccg tgacagacga 2521 tgggtggctc ctgttgtcca ccgcgtacaa atgtttgggc caaagtcttg tcagccttgc 2581 ttgcgaacct aattcccaat tttgtcactt cgcaccccca ttgatcgagc cctaacccct 2641 gcccatcagg caatccaatt aagctcgcat tgtctgcctt gtttagtttg gctcctgccc 2701 gtttcggcgt ccacttgcac aaacacaaac aagcattata tataaggctc gtctctccct 2761 cccaaccaca ctcacttttt tgcccgtctt cccttgctaa cacaaaagtc aagaacacaa 2821 acaaccaccc caaccccctt acacacaaga catatctaca gcaatgggta aaaagcctga 2881 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct 2941 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg 3001 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg 3061 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga 3121 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga 3181 aaccgaactg cccgctgttc tgcagccggt cgcggaggca atggatgcca ttgctgcggc 3241 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac 3301 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt 3361 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc 3421 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct 3481 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc 3541 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca 3601 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta 3661 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga 3721 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg 3781 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact 3841 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat aaccccacgt 3901 tgccggtctt gcctcctact acctgtccat caatgacgag gttctcaccc ctgcccaggt 3961 cgaggctctt attactgagt ccaacaccgg tgttcttccc accaccaacc tcaagggctc 4021 tcccaacgct gttgcctaca acggtgttgg catttaggca attaacagat agtttgccgg 4081 tgataattct cttaacctcc cacactcctt tgacataacg atttatgtaa cgaaactgaa 4141 atttgaccag atattgttgt aaatagaaaa tctggcttgt aggtggcaaa atgcggcgtc 4201 tttgttcatc aattccctct gtgactactc gtcatccctt tatgttcgac tgtcgtattt 4261 cttattttcc atacatatgc aagtgagatg cccgtgtccg aattcataac ttcgtataat 4321 gtatgctata cgaagttatg tgatatcaga tccactagtg gcctccgagc gtcgacaagc 4381 atacagccct cgggtggttg tgtcgaaata caacagccag tcctccacga tccagctcag 4441 atagccctgg gtgttgagat agagacaagt cgagcgagat gttctcagtg gacttgagtt 4501 tgatgttgtg aaagatggtt ggattgagag cacaactcca tagaacatag tagtctcagt 4561 ccatgagcta ggagagaatc tccagtgctg atgtcatttg aagaagaaga ggaagaagaa 4621 gacagataga tcaactcagc cccttccagt tgccgtacaa tgactcaacc aaatacgttc 4681 aagcctgctt tcaagcctgc gtgctctttc tgtctcatta gttgaacgta gatcatctga 4741 gagaacttgg cagaccgtgc aaacacaagg gcgagactga taggacactt gatctgatcc 4801 aaaatttgac agaggaaaac tagttttgaa gttactgaac gtttcttctc caattgcttc 4861 taacatcgcg tgcattcgcg gccgcattta aatccccact tcagaagttc ctatactttc 4921 tagagaatag gaacttcact atagagtcga ataagggcga caccccataa ttagcccggg 4981 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc agttccctac 5041 tctcgcatgg ggagtcccca cactaccatc ggcgctacgg cgtttcactt ctgagttcgg 5101 catggggtca ggtgggacca ccgcgctact gccgccaggc aaacaagggg tgttatgagc 5161 catattcagg tataaatggg ctcgcgataa tgttcagaat tggttaattg gttgtaacac 5221 tgacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 5281 aaccctgata aatgcttcaa taatattgaa aaaggaagaa tatgagtatt caacatttcc 5341 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 5401 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 5461 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 5521 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 5581 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 5641 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 5701 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 5761 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 5821 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagcg atggcaacaa 5881 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 5941 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 6001 ggtttattgc tgataaatcc ggagccggtg agcgtggttc tcgcggtatc atcgcagcgc 6061 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 6121 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 6181 aaaagcagag cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta 6241 acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 6301 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 6361 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 6421 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttagcccac 6481 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 6541 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 6601 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 6661 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 6721 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 6781 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 6841 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 6901 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 6961 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 7021 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaggcgag 7081 agtagggaac tgccaggcat caaactaagc agaaggcccc tgacggatgg cctttttgcg 7141 tttctacaaa ctctttctgt gttgtaaaac gacggccagt cttaagctcg ggccccctgg 7201 gcggttctga taacgagtaa tcgttaatcc gcaaataacg taaaaacccg cttcggcggg 7261 tttttttatg gggggagttt agggaaagag catttgtcag aatatttaag ggcgcctgtc 7321 actttgcttg atatatgaga attatttaac cttataaatg agaaaaaagc aacgcacttt 7381 aaataagata cgttgctttt tcgattgatg aacacctata attaaactat tcatctatta 7441 tttatgattt tttgtatata caatatttct agtttgttaa agagaattaa gaaaataaat 7501 ctcgaaaata ataaagggaa aatcagtttt tgatatcaaa attatacatg tcaacgataa 7561 tacaaaatat aatacaaact ataagatgtt atcagtattt attatgcatt tagaataaat 7621 tttgtgtcgc ccttattcga ctcactatag aagttcctat tctctagaaa gtataggaac 7681 ttcacttcat tttatttaaa tttgcggccg c //