LOCUS       Exported                7715 bp ds-DNA     circular SYN 20-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCfB6575 (pIntE_2-HphMx-TPex20-TLip2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2017 from SnapGene 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    14..513
                     /label=IntE-2 UP
     terminator      complement(527..839)
                     /label=TPex20
     misc_feature    840..868
                     /label=USER cloning site
     terminator      869..1805
                     /label=T lip2
     misc_feature    1828..1861
                     /label=loxP
     promoter        1862..2863
                     /label=PrExp1
     CDS             2864..3892
                     /label=HphSyn
     terminator      3893..4305
                     /label=Ttef
                     /label=Tcyc1
     misc_feature    4306..4339
                     /label=loxP
     misc_feature    4365..4868
                     /label=IntE-2 DW
     misc_feature    5326..6186
                     /label=AmpR
     misc_feature    complement(6287..6930)
                     /label=pUC ORI
ORIGIN
        1 gaatgcgtgc gatatggtgc caaatcgctt gtcactttga cacaatgaag atattcgatt
       61 cagtcgatac catggccgtc aactttcttg tccgacctct caactttgta tctatgtctc
      121 aggacggagt gttggtccat ccagccccta ccagttccgt ggtgatgttg aaagggctat
      181 ttttatccag atcagagggt cactggagct tgaataggat ccacaagaag ttcaaaggac
      241 tttactggtg tttcttttgt ccgagaactt caacaactcc gtctggtgtc tccaagatag
      301 attctggtgc tctccacgcc tatacatctc caagtactgt acaccacaaa caccttccgg
      361 gtggttgctt acagtttcaa taggcgaccg cttgatttcg gcaaatctac acctgccagc
      421 tacgcatatt gcaggaagca gcatcatgga atggactgta tagccataag acaagaatgc
      481 tctggagaaa tcgtccgatt ttgctgcact aagacgtgca acgcttacgc aactaacatg
      541 aatgaatacg atatacatca aagactatga tacgcagtat tgcacactgt acgagtaagg
      601 gcactagcca ctgcactcaa gtgaaaccgt tgcccgggta cgagtatgag tatgtacagt
      661 atgtttagta ttgtacttgg acaatgcttg tatcgtacat tctcaagtgt caaacataaa
      721 tatccgttgc tatatcctcg caccaccacg tagctcgcta tatccctgtg ttgaatccat
      781 ccatcttgga ttgccaattg tgcacacaga accgggcact cacttcccca tccacacttc
      841 aacggaatgc gtgcgatcgc gtgcattcct tctgttcgga atcaacctca aggttaacgg
      901 ccacgatccc ctcgttgtta ctcttggtca gcccattgtc ggtaacgctg gctttgctaa
      961 ctgggtcgat aaactcttct ttggccagga gaaccccgat gtctccaagg tgtccaaaga
     1021 ccgaaagctc taccgaatca cccaccgagg agatatcgtc cctcaagtgc ccttctggga
     1081 cggttaccag cactgctctg gtgaggtctt tattgactgg cccctgatcc accctcctct
     1141 ctccaacgtt gtcatgtgcc agggccagag caataaacag tgctctgccg gtaacactct
     1201 gctccagcag gtcaatgtga ttggaaacca tctgcagtac ttcgtcaccg agggtgtctg
     1261 tggtatctaa gctatttatc actctttaca acttctacct caactatcta ctttaataaa
     1321 tgaatatcgt ttattctcta tgattactgt atatgcgttc ctctaagaca aatcgaaacc
     1381 agcatgcgat cgaatggcat acaaaagttt cttccgaagt tgatcaatgt cctgatagtc
     1441 aggcagcttg agaagattga cacaggtgga ggccgtaggg aaccgatcaa cctgtctacc
     1501 agcgttacga atggcaaatg acgggttcaa agccttgaat ccttgcaatg gtgccttgga
     1561 tactgatgtc acaaacttaa gaagcagccg cttgtcctct tcctcgaaac tctcaaacac
     1621 agtccagaag tcctttatag tttgatctgt atccagatag cctccgtaat tggtgtgtgt
     1681 cttcaaatcc cagacgtcca cattggcatg tcctccactg ataagcattt gaagttcatc
     1741 tgcgttgaac attgagaccc acgaagggtc aatgagctgg tatagaccgc ccaagaatgc
     1801 atctgagaac agctgaagct tcgtacgata acttcgtata atgtatgcta tacgaagtta
     1861 taaggagttt ggcgcccgtt ttttcgagcc ccacacgttt cggtgagtat gagcggcggc
     1921 agattcgagc gtttccggtt tccgcggctg gacgagagcc catgatgggg gctcccacca
     1981 ccagcaatca gggccctgat tacacaccca cctgtaatgt catgctgttc atcgtggtta
     2041 atgctgctgt gtgctgtgtg tgtgtgttgt ttggcgctca ttgttgcgtt atgcagcgta
     2101 caccacaata ttggaagctt attagccttt ctattttttc gtttgcaagg cttaacaaca
     2161 ttgctgtgga gagggatggg gatatggagg ccgctggagg gagtcggaga ggcgttttgg
     2221 agcggcttgg cctggcgccc agctcgcgaa acgcacctag gaccctttgg cacgccgaaa
     2281 tgtgccactt ttcagtctag taacgcctta cctacgtcat tccatgcatg catgtttgcg
     2341 ccttttttcc cttgcccttg atcgccacac agtacagtgc actgtacagt ggaggttttg
     2401 ggggggtctt agatgggagc taaaagcggc ctagcggtac actagtggga ttgtatggag
     2461 tggcatggag cctaggtgga gcctgacagg acgcacgacc ggctagcccg tgacagacga
     2521 tgggtggctc ctgttgtcca ccgcgtacaa atgtttgggc caaagtcttg tcagccttgc
     2581 ttgcgaacct aattcccaat tttgtcactt cgcaccccca ttgatcgagc cctaacccct
     2641 gcccatcagg caatccaatt aagctcgcat tgtctgcctt gtttagtttg gctcctgccc
     2701 gtttcggcgt ccacttgcac aaacacaaac aagcattata tataaggctc gtctctccct
     2761 cccaaccaca ctcacttttt tgcccgtctt cccttgctaa cacaaaagtc aagaacacaa
     2821 acaaccaccc caaccccctt acacacaaga catatctaca gcaatgggta aaaagcctga
     2881 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     2941 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     3001 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     3061 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     3121 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     3181 aaccgaactg cccgctgttc tgcagccggt cgcggaggca atggatgcca ttgctgcggc
     3241 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     3301 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     3361 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     3421 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     3481 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     3541 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     3601 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     3661 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     3721 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     3781 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     3841 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat aaccccacgt
     3901 tgccggtctt gcctcctact acctgtccat caatgacgag gttctcaccc ctgcccaggt
     3961 cgaggctctt attactgagt ccaacaccgg tgttcttccc accaccaacc tcaagggctc
     4021 tcccaacgct gttgcctaca acggtgttgg catttaggca attaacagat agtttgccgg
     4081 tgataattct cttaacctcc cacactcctt tgacataacg atttatgtaa cgaaactgaa
     4141 atttgaccag atattgttgt aaatagaaaa tctggcttgt aggtggcaaa atgcggcgtc
     4201 tttgttcatc aattccctct gtgactactc gtcatccctt tatgttcgac tgtcgtattt
     4261 cttattttcc atacatatgc aagtgagatg cccgtgtccg aattcataac ttcgtataat
     4321 gtatgctata cgaagttatg tgatatcaga tccactagtg gcctgtgtgg attgcgatat
     4381 tgaagtgttg actcgaagag acagctgcct gtcaagttga ttgttagctc cttggcatac
     4441 agatgcaaga aatgagagtg gccatacggc ggcagttagc ataagatact acttgtagga
     4501 gagagcgctc accaccagct aaatttagga ccctggctgt ctatttacat ttttgcaccc
     4561 tgttggctgc ctagctatgc ggccagtcag cccgctggac atgtgtttgg catgtgtttg
     4621 gcatgtgttt ggtgcaggga gaaccgcgat ggtgaccgtc caagcaatta cccgtttgga
     4681 ccagaagagt aaagagcgac atttgcaaaa gaaacgcgct gaaaaagcaa ctccagcgat
     4741 gaggaactga gagtctgaag tctctggacg aggagtggga ggggggatgc cttctgtggg
     4801 tgccgtacgt gtctaccgag aagttaacaa ccctcctgtt ggtatagtct acaaacctgg
     4861 cgtatttcat cgcgtgcatt cgcggccgca tttaaatccc cacttcagaa gttcctatac
     4921 tttctagaga ataggaactt cactatagag tcgaataagg gcgacacccc ataattagcc
     4981 cgggcgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggcagttcc
     5041 ctactctcgc atggggagtc cccacactac catcggcgct acggcgtttc acttctgagt
     5101 tcggcatggg gtcaggtggg accaccgcgc tactgccgcc aggcaaacaa ggggtgttat
     5161 gagccatatt caggtataaa tgggctcgcg ataatgttca gaattggtta attggttgta
     5221 acactgaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5281 caataaccct gataaatgct tcaataatat tgaaaaagga agaatatgag tattcaacat
     5341 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5401 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5461 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5521 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5581 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5641 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5701 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5761 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5821 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcgatggca
     5881 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5941 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6001 ggctggttta ttgctgataa atccggagcc ggtgagcgtg gttctcgcgg tatcatcgca
     6061 gcgctggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6121 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6181 tggtaaaagc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     6241 cttaacgtga gttacgcgcg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6301 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6361 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6421 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagc
     6481 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6541 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6601 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6661 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6721 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6781 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6841 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6901 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6961 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7021 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaagg
     7081 cgagagtagg gaactgccag gcatcaaact aagcagaagg cccctgacgg atggcctttt
     7141 tgcgtttcta caaactcttt ctgtgttgta aaacgacggc cagtcttaag ctcgggcccc
     7201 ctgggcggtt ctgataacga gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg
     7261 cgggtttttt tatgggggga gtttagggaa agagcatttg tcagaatatt taagggcgcc
     7321 tgtcactttg cttgatatat gagaattatt taaccttata aatgagaaaa aagcaacgca
     7381 ctttaaataa gatacgttgc tttttcgatt gatgaacacc tataattaaa ctattcatct
     7441 attatttatg attttttgta tatacaatat ttctagtttg ttaaagagaa ttaagaaaat
     7501 aaatctcgaa aataataaag ggaaaatcag tttttgatat caaaattata catgtcaacg
     7561 ataatacaaa atataataca aactataaga tgttatcagt atttattatg catttagaat
     7621 aaattttgtg tcgcccttat tcgactcact atagaagttc ctattctcta gaaagtatag
     7681 gaacttcact tcattttatt taaatttgcg gccgc
//
