LOCUS       Exported                7749 bp ds-DNA     circular SYN 20-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCfB6576 (pIntE-3-HphMx-TPex20-TLip2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2017 from SnapGene 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    14..536
                     /label=IntE_3 UP
     terminator      complement(550..862)
                     /label=TPex20
     misc_feature    863..891
                     /label=USER cloning site
     terminator      892..1828
                     /label=T lip2
     misc_feature    1851..1884
                     /label=loxP
     promoter        1885..2886
                     /label=PrExp1
     CDS             2887..3915
                     /label=HphSyn
     terminator      3916..4329
                     /label=Ttef
                     /label=Tcyc1
     misc_feature    4330..4363
                     /label=loxP
     misc_feature    4389..4902
                     /label=IntE-3 DW
     misc_feature    5360..6220
                     /label=AmpR
     misc_feature    complement(6321..6964)
                     /label=pUC ORI
ORIGIN
        1 gaatgcgtgc gattaacttt tttcgtgact ctgttctcca cggtcaggtg tcgtatacgt
       61 aagtactgat actcgctcgt actggtactg tgcggtacat acacagtacg ttggcaccag
      121 tgacaattgt gctatcgata ctgttataag cggcttctat ccatggttta ctgaggagaa
      181 atgttctagc agtgacgtta caagtatcgt cactacatta tgttgccatg ttcgcacaat
      241 acaacataca gacccgtttt caatgcgacg tatacgttca ggtaccttca gtgcatgtgt
      301 atgtaccaca ttatacgaaa cagctcgcac tatataccat gcaacttgta caagtatggg
      361 ccacattgtg acaccaatac agaacgcaca aacggtacac cgacgcagga agtttggaag
      421 agcgttcatg ccgattgtgt actcacagta ccagtacaat acaccggtac ttcttccctg
      481 tatcgtacga acttgtactc ctagtctaaa tgacctcaaa ctcaagacat caaacaacgt
      541 gcaacgctta cgcaactaac atgaatgaat acgatataca tcaaagacta tgatacgcag
      601 tattgcacac tgtacgagta agggcactag ccactgcact caagtgaaac cgttgcccgg
      661 gtacgagtat gagtatgtac agtatgttta gtattgtact tggacaatgc ttgtatcgta
      721 cattctcaag tgtcaaacat aaatatccgt tgctatatcc tcgcaccacc acgtagctcg
      781 ctatatccct gtgttgaatc catccatctt ggattgccaa ttgtgcacac agaaccgggc
      841 actcacttcc ccatccacac ttcaacggaa tgcgtgcgat cgcgtgcatt ccttctgttc
      901 ggaatcaacc tcaaggttaa cggccacgat cccctcgttg ttactcttgg tcagcccatt
      961 gtcggtaacg ctggctttgc taactgggtc gataaactct tctttggcca ggagaacccc
     1021 gatgtctcca aggtgtccaa agaccgaaag ctctaccgaa tcacccaccg aggagatatc
     1081 gtccctcaag tgcccttctg ggacggttac cagcactgct ctggtgaggt ctttattgac
     1141 tggcccctga tccaccctcc tctctccaac gttgtcatgt gccagggcca gagcaataaa
     1201 cagtgctctg ccggtaacac tctgctccag caggtcaatg tgattggaaa ccatctgcag
     1261 tacttcgtca ccgagggtgt ctgtggtatc taagctattt atcactcttt acaacttcta
     1321 cctcaactat ctactttaat aaatgaatat cgtttattct ctatgattac tgtatatgcg
     1381 ttcctctaag acaaatcgaa accagcatgc gatcgaatgg catacaaaag tttcttccga
     1441 agttgatcaa tgtcctgata gtcaggcagc ttgagaagat tgacacaggt ggaggccgta
     1501 gggaaccgat caacctgtct accagcgtta cgaatggcaa atgacgggtt caaagccttg
     1561 aatccttgca atggtgcctt ggatactgat gtcacaaact taagaagcag ccgcttgtcc
     1621 tcttcctcga aactctcaaa cacagtccag aagtccttta tagtttgatc tgtatccaga
     1681 tagcctccgt aattggtgtg tgtcttcaaa tcccagacgt ccacattggc atgtcctcca
     1741 ctgataagca tttgaagttc atctgcgttg aacattgaga cccacgaagg gtcaatgagc
     1801 tggtatagac cgcccaagaa tgcatctgag aacagctgaa gcttcgtacg ataacttcgt
     1861 ataatgtatg ctatacgaag ttataaggag tttggcgccc gttttttcga gccccacacg
     1921 tttcggtgag tatgagcggc ggcagattcg agcgtttccg gtttccgcgg ctggacgaga
     1981 gcccatgatg ggggctccca ccaccagcaa tcagggccct gattacacac ccacctgtaa
     2041 tgtcatgctg ttcatcgtgg ttaatgctgc tgtgtgctgt gtgtgtgtgt tgtttggcgc
     2101 tcattgttgc gttatgcagc gtacaccaca atattggaag cttattagcc tttctatttt
     2161 ttcgtttgca aggcttaaca acattgctgt ggagagggat ggggatatgg aggccgctgg
     2221 agggagtcgg agaggcgttt tggagcggct tggcctggcg cccagctcgc gaaacgcacc
     2281 taggaccctt tggcacgccg aaatgtgcca cttttcagtc tagtaacgcc ttacctacgt
     2341 cattccatgc atgcatgttt gcgccttttt tcccttgccc ttgatcgcca cacagtacag
     2401 tgcactgtac agtggaggtt ttgggggggt cttagatggg agctaaaagc ggcctagcgg
     2461 tacactagtg ggattgtatg gagtggcatg gagcctaggt ggagcctgac aggacgcacg
     2521 accggctagc ccgtgacaga cgatgggtgg ctcctgttgt ccaccgcgta caaatgtttg
     2581 ggccaaagtc ttgtcagcct tgcttgcgaa cctaattccc aattttgtca cttcgcaccc
     2641 ccattgatcg agccctaacc cctgcccatc aggcaatcca attaagctcg cattgtctgc
     2701 cttgtttagt ttggctcctg cccgtttcgg cgtccacttg cacaaacaca aacaagcatt
     2761 atatataagg ctcgtctctc cctcccaacc acactcactt ttttgcccgt cttcccttgc
     2821 taacacaaaa gtcaagaaca caaacaacca ccccaacccc cttacacaca agacatatct
     2881 acagcaatgg gtaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     2941 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     3001 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     3061 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     3121 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     3181 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     3241 gcaatggatg ccattgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     3301 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     3361 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     3421 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     3481 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     3541 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     3601 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     3661 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     3721 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     3781 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     3841 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     3901 agggcaaagg aataacccca cgttgccggt cttgcctcct actacctgtc catcaatgac
     3961 gaggttctca cccctgccca ggtcgaggct cttattactg agtccaacac cggtgttctt
     4021 cccaccacca acctcaaggg ctctcccaac gctgttgcct acaacggtgt tggcatttag
     4081 gcaattaaca gatagtttgc cggtgataat tctcttaacc tcccacactc ctttgacata
     4141 acgatttatg taacgaaact gaaatttgac cagatattgt tgtaaataga aaatctggct
     4201 tgtaggtggc aaaatgcggc gtctttgttc atcaattccc tctgtgacta ctcgtcatcc
     4261 ctttatgttc gactgtcgta tttcttattt tccatacata tgcaagtgag atgcccgtgt
     4321 ccgaattcga taacttcgta taatgtatgc tatacgaagt tatgtgatat cagatccact
     4381 agtggcctga actgtatacc caatgtactt gtacgccttg aatcagtcga tggtctggca
     4441 cagaaatgac cacacatacc cagttaacta agccattttc ttcatcagcg atggaaatgc
     4501 atttaaagat ggttctattt catacaacta cccattgact aatggttatt cttgtaaaat
     4561 gatggagaac ttctactaca agtacaaata ctacacgcga agttccccag attgcaccgc
     4621 cacaaacacc aatcgaaggt ttctctccat acatttttcc catttgggac ctgtgcataa
     4681 ttataattca tcaccataaa aatttcctgt tgcataacaa cctgactcct gaagtttcgc
     4741 gcatgtgtac atattgctcc atcttcaagt acagtacagg cacagtaagg tgacacgtag
     4801 tagaacagtc aaactaacac atcaggccca ccatccacct tacacgtacg gtacttgtag
     4861 aaaagcgctt cgttcaattc aggttttagg catttccttc acatcgcgtg cattcgcggc
     4921 cgcatttaaa tccccacttc agaagttcct atactttcta gagaatagga acttcactat
     4981 agagtcgaat aagggcgaca ccccataatt agcccgggcg aaaggcccag tctttcgact
     5041 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcatgggg agtccccaca
     5101 ctaccatcgg cgctacggcg tttcacttct gagttcggca tggggtcagg tgggaccacc
     5161 gcgctactgc cgccaggcaa acaaggggtg ttatgagcca tattcaggta taaatgggct
     5221 cgcgataatg ttcagaattg gttaattggt tgtaacactg acccctattt gtttattttt
     5281 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5341 atattgaaaa aggaagaata tgagtattca acatttccgt gtcgccctta ttcccttttt
     5401 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5461 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5521 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5581 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5641 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5701 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5761 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5821 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5881 cgagcgtgac accacgatgc ctgtagcgat ggcaacaacg ttgcgcaaac tattaactgg
     5941 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6001 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatccgg
     6061 agccggtgag cgtggttctc gcggtatcat cgcagcgctg gggccagatg gtaagccctc
     6121 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6181 gatcgctgag ataggtgcct cactgattaa gcattggtaa aagcagagca ttacgctgac
     6241 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgcgcgtcgt
     6301 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6361 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6421 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6481 caaatactgt tcttctagtg tagccgtagt tagcccacca cttcaagaac tctgtagcac
     6541 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6601 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6661 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6721 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6781 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6841 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6901 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6961 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7021 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7081 agcgcagcga gtcagtgagc gaggaagcgg aaggcgagag tagggaactg ccaggcatca
     7141 aactaagcag aaggcccctg acggatggcc tttttgcgtt tctacaaact ctttctgtgt
     7201 tgtaaaacga cggccagtct taagctcggg ccccctgggc ggttctgata acgagtaatc
     7261 gttaatccgc aaataacgta aaaacccgct tcggcgggtt tttttatggg gggagtttag
     7321 ggaaagagca tttgtcagaa tatttaaggg cgcctgtcac tttgcttgat atatgagaat
     7381 tatttaacct tataaatgag aaaaaagcaa cgcactttaa ataagatacg ttgctttttc
     7441 gattgatgaa cacctataat taaactattc atctattatt tatgattttt tgtatataca
     7501 atatttctag tttgttaaag agaattaaga aaataaatct cgaaaataat aaagggaaaa
     7561 tcagtttttg atatcaaaat tatacatgtc aacgataata caaaatataa tacaaactat
     7621 aagatgttat cagtatttat tatgcattta gaataaattt tgtgtcgccc ttattcgact
     7681 cactatagaa gttcctattc tctagaaagt ataggaactt cacttcattt tatttaaatt
     7741 tgcggccgc
//
