LOCUS       Exported                7731 bp ds-DNA     circular SYN 20-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCfB5219 (pIntE-4-HphMx-TPex20-TLip2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2017 from SnapGene 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    458..1318
                     /label=AmpR
     misc_feature    complement(1419..2062)
                     /label=pUC ORI
     misc_feature    2861..3380
                     /label=IntE-4_UP
     terminator      complement(3394..3706)
                     /label=TPex20
     misc_feature    3707..3735
                     /label=USER cloning site
     terminator      3736..4672
                     /label=T lip2
     misc_feature    4695..4728
                     /label=loxP
     promoter        4729..5730
                     /label=PrExp1
     CDS             5731..6759
                     /label=HphSyn
     terminator      6760..7172
                     /label=TTef
     misc_feature    7173..7206
                     /label=loxP
     misc_feature    7232..7731
                     /label=IntE-4_DW
ORIGIN
        1 atcgcgtgca ttcgcggccg catttaaatc cccacttcag aagttcctat actttctaga
       61 gaataggaac ttcactatag agtcgaataa gggcgacacc ccataattag cccgggcgaa
      121 aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
      181 gcatggggag tccccacact accatcggcg ctacggcgtt tcacttctga gttcggcatg
      241 gggtcaggtg ggaccaccgc gctactgccg ccaggcaaac aaggggtgtt atgagccata
      301 ttcaggtata aatgggctcg cgataatgtt cagaattggt taattggttg taacactgac
      361 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      421 ctgataaatg cttcaataat attgaaaaag gaagaatatg agtattcaac atttccgtgt
      481 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      541 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      601 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      661 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
      721 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      781 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      841 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
      901 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
      961 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcgatgg caacaacgtt
     1021 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     1081 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     1141 tattgctgat aaatccggag ccggtgagcg tggttctcgc ggtatcatcg cagcgctggg
     1201 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     1261 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaaaa
     1321 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     1381 gagttacgcg cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1441 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1501 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1561 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta gcccaccact
     1621 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1681 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1741 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1801 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1861 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1921 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1981 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2041 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2101 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2161 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ggcgagagta
     2221 gggaactgcc aggcatcaaa ctaagcagaa ggcccctgac ggatggcctt tttgcgtttc
     2281 tacaaactct ttctgtgttg taaaacgacg gccagtctta agctcgggcc ccctgggcgg
     2341 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
     2401 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaagggcg cctgtcactt
     2461 tgcttgatat atgagaatta tttaacctta taaatgagaa aaaagcaacg cactttaaat
     2521 aagatacgtt gctttttcga ttgatgaaca cctataatta aactattcat ctattattta
     2581 tgattttttg tatatacaat atttctagtt tgttaaagag aattaagaaa ataaatctcg
     2641 aaaataataa agggaaaatc agtttttgat atcaaaatta tacatgtcaa cgataataca
     2701 aaatataata caaactataa gatgttatca gtatttatta tgcatttaga ataaattttg
     2761 tgtcgccctt attcgactca ctatagaagt tcctattctc tagaaagtat aggaacttca
     2821 cttcatttta tttaaatttg cggccgcgaa tgcgtgcgat gcggaggagc aatagacata
     2881 cgatttgaca ttttgacatt tcacagtatc ggcagccttg ctatagcaag tgtgtatttg
     2941 tagatagaga caggcaatta gatttggagg ggaccgcaag aaacagatca tcattctgtc
     3001 ggcccctgtt gtccctgtgg ctctgttagt ccgctggtcc gtggccggag cttgtcatgt
     3061 ttctggaata ccctctggcc acattaactc cgctaaagcc tcaaggccgt aaaggcgcca
     3121 gtggcatgtt gcaagcaaag ccgcacgcat aacacgccac atcacgtgag cggtcatagg
     3181 ccggtcgcca gacggctaca accctattac gcgggtgctc tggccaaaca gaccgtggag
     3241 gaactcttcg acacttgata ttaaactcta tgccacttat cgggcctact gatcagactc
     3301 aaggtctatt gttctgagag aggaagtttg gggagaaaga acgggcacat gtgttgatct
     3361 ccatcaccgt ggggagggga acgtgcaacg cttacgcaac taacatgaat gaatacgata
     3421 tacatcaaag actatgatac gcagtattgc acactgtacg agtaagggca ctagccactg
     3481 cactcaagtg aaaccgttgc ccgggtacga gtatgagtat gtacagtatg tttagtattg
     3541 tacttggaca atgcttgtat cgtacattct caagtgtcaa acataaatat ccgttgctat
     3601 atcctcgcac caccacgtag ctcgctatat ccctgtgttg aatccatcca tcttggattg
     3661 ccaattgtgc acacagaacc gggcactcac ttccccatcc acacttcaac ggaatgcgtg
     3721 cgatcgcgtg cattccttct gttcggaatc aacctcaagg ttaacggcca cgatcccctc
     3781 gttgttactc ttggtcagcc cattgtcggt aacgctggct ttgctaactg ggtcgataaa
     3841 ctcttctttg gccaggagaa ccccgatgtc tccaaggtgt ccaaagaccg aaagctctac
     3901 cgaatcaccc accgaggaga tatcgtccct caagtgccct tctgggacgg ttaccagcac
     3961 tgctctggtg aggtctttat tgactggccc ctgatccacc ctcctctctc caacgttgtc
     4021 atgtgccagg gccagagcaa taaacagtgc tctgccggta acactctgct ccagcaggtc
     4081 aatgtgattg gaaaccatct gcagtacttc gtcaccgagg gtgtctgtgg tatctaagct
     4141 atttatcact ctttacaact tctacctcaa ctatctactt taataaatga atatcgttta
     4201 ttctctatga ttactgtata tgcgttcctc taagacaaat cgaaaccagc atgcgatcga
     4261 atggcataca aaagtttctt ccgaagttga tcaatgtcct gatagtcagg cagcttgaga
     4321 agattgacac aggtggaggc cgtagggaac cgatcaacct gtctaccagc gttacgaatg
     4381 gcaaatgacg ggttcaaagc cttgaatcct tgcaatggtg ccttggatac tgatgtcaca
     4441 aacttaagaa gcagccgctt gtcctcttcc tcgaaactct caaacacagt ccagaagtcc
     4501 tttatagttt gatctgtatc cagatagcct ccgtaattgg tgtgtgtctt caaatcccag
     4561 acgtccacat tggcatgtcc tccactgata agcatttgaa gttcatctgc gttgaacatt
     4621 gagacccacg aagggtcaat gagctggtat agaccgccca agaatgcatc tgagaacagc
     4681 tgaagcttcg tacgataact tcgtataatg tatgctatac gaagttataa ggagtttggc
     4741 gcccgttttt tcgagcccca cacgtttcgg tgagtatgag cggcggcaga ttcgagcgtt
     4801 tccggtttcc gcggctggac gagagcccat gatgggggct cccaccacca gcaatcaggg
     4861 ccctgattac acacccacct gtaatgtcat gctgttcatc gtggttaatg ctgctgtgtg
     4921 ctgtgtgtgt gtgttgtttg gcgctcattg ttgcgttatg cagcgtacac cacaatattg
     4981 gaagcttatt agcctttcta ttttttcgtt tgcaaggctt aacaacattg ctgtggagag
     5041 ggatggggat atggaggccg ctggagggag tcggagaggc gttttggagc ggcttggcct
     5101 ggcgcccagc tcgcgaaacg cacctaggac cctttggcac gccgaaatgt gccacttttc
     5161 agtctagtaa cgccttacct acgtcattcc atgcatgcat gtttgcgcct tttttccctt
     5221 gcccttgatc gccacacagt acagtgcact gtacagtgga ggttttgggg gggtcttaga
     5281 tgggagctaa aagcggccta gcggtacact agtgggattg tatggagtgg catggagcct
     5341 aggtggagcc tgacaggacg cacgaccggc tagcccgtga cagacgatgg gtggctcctg
     5401 ttgtccaccg cgtacaaatg tttgggccaa agtcttgtca gccttgcttg cgaacctaat
     5461 tcccaatttt gtcacttcgc acccccattg atcgagccct aacccctgcc catcaggcaa
     5521 tccaattaag ctcgcattgt ctgccttgtt tagtttggct cctgcccgtt tcggcgtcca
     5581 cttgcacaaa cacaaacaag cattatatat aaggctcgtc tctccctccc aaccacactc
     5641 acttttttgc ccgtcttccc ttgctaacac aaaagtcaag aacacaaaca accaccccaa
     5701 cccccttaca cacaagacat atctacagca atgggtaaaa agcctgaact caccgcgacg
     5761 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     5821 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     5881 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     5941 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     6001 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     6061 gctgttctgc agccggtcgc ggaggcaatg gatgccattg ctgcggccga tcttagccag
     6121 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     6181 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     6241 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     6301 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     6361 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     6421 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     6481 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     6541 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     6601 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     6661 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     6721 aaccgacgcc ccagcactcg tccgagggca aaggaataac cccacgttgc cggtcttgcc
     6781 tcctactacc tgtccatcaa tgacgaggtt ctcacccctg cccaggtcga ggctcttatt
     6841 actgagtcca acaccggtgt tcttcccacc accaacctca agggctctcc caacgctgtt
     6901 gcctacaacg gtgttggcat ttaggcaatt aacagatagt ttgccggtga taattctctt
     6961 aacctcccac actcctttga cataacgatt tatgtaacga aactgaaatt tgaccagata
     7021 ttgttgtaaa tagaaaatct ggcttgtagg tggcaaaatg cggcgtcttt gttcatcaat
     7081 tccctctgtg actactcgtc atccctttat gttcgactgt cgtatttctt attttccata
     7141 catatgcaag tgagatgccc gtgtccgaat tcataacttc gtataatgta tgctatacga
     7201 agttatgtga tatcagatcc actagtggcc tcaccgaggg atagggaaca ccgcggaaca
     7261 gggaacatcg actctgagac agaggtgacc tgacgagcaa cgtatgcgag caactcgtat
     7321 ctgcagttgg ttggcctggt cactcgatta attggccatc cgaaagcacc attagcctat
     7381 ctaacgcact aaaatggtgg ggaagtcaaa ggaccatggt tcgttcattg ttggctaacg
     7441 gcccgaaatc cgccaaagtg tgggaaaaaa acataaaaaa tatataaaaa aaagttgctt
     7501 tggattttgg gatgcagcaa ccaaaaactc gcgcaaaagc ccgctcgagt gtctctgaac
     7561 gcaaatacga ccgacgcggt ctccaacatc tcgtcgcggt cccctccccc cttcagccag
     7621 cgtccctcca gacccaggcc acagcataat atttcagagc gttgacaccc agagcggggg
     7681 agctcgtaat aaggtacgcc tgtgcagctg ggcagtacgg tccagtgtta a
//
