LOCUS       Exported                6612 bp ds-DNA     linear   SYN 20-DEC-2017
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCfB4788 (pIntA-1-Nat-TPex20-TLip2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2017 from SnapGene 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    14..504
                     /label=IntA-1 UP
     terminator      complement(518..830)
                     /label=TPex20
     misc_feature    831..859
                     /label=USER cloning site
     terminator      860..1796
                     /label=T lip2
     misc_feature    1819..1852
                     /label=loxP
     promoter        1853..2383
                     /label=PrTefintron
     CDS             2384..2953
                     /label=Nat
                     /label=Nat codon optimized
     terminator      2954..3186
                     /label=Tcyc
     misc_feature    3187..3220
                     /label=loxP
     misc_feature    3246..3765
                     /label=IntA_1 DW
     misc_feature    4223..5083
                     /label=AmpR
     misc_feature    complement(5184..5827)
                     /label=pUC ORI
ORIGIN
        1 gaatgcgtgc gatttattat cccaaaaata ccccactctc cattatttcc gactgtgcag
       61 attcccatat caacccaatc tccatcaaaa caccgcagcc aaactctggc aaaagtgcaa
      121 catgcgatac ggacacggtg ggcggtgggc atcgatcaac tggccgtagg tgcatctggg
      181 ctggaattac cccacagtgc agtgctaagc agtggtgatg gaggatattg ggggatggaa
      241 gatattgggg gatggaggga gaggactaga cctctcagta acagtgcaag cagtacatgc
      301 aatacaagta acacccacca acacacggac cagacgagac caaccaactg cacagcccta
      361 ccaggaaacg gcagagcttg tggacacaac agactgcgac aagggacccg ctttgccagt
      421 gtgatgcagg aaccatactc ttttcctttt ttcggacagc cggcacacga gcttgacgat
      481 caaaccatat cggccgagtg cgacacgtgc aacgcttacg caactaacat gaatgaatac
      541 gatatacatc aaagactatg atacgcagta ttgcacactg tacgagtaag ggcactagcc
      601 actgcactca agtgaaaccg ttgcccgggt acgagtatga gtatgtacag tatgtttagt
      661 attgtacttg gacaatgctt gtatcgtaca ttctcaagtg tcaaacataa atatccgttg
      721 ctatatcctc gcaccaccac gtagctcgct atatccctgt gttgaatcca tccatcttgg
      781 attgccaatt gtgcacacag aaccgggcac tcacttcccc atccacactt caacggaatg
      841 cgtgcgatcg cgtgcattcc ttctgttcgg aatcaacctc aaggttaacg gccacgatcc
      901 cctcgttgtt actcttggtc agcccattgt cggtaacgct ggctttgcta actgggtcga
      961 taaactcttc tttggccagg agaaccccga tgtctccaag gtgtccaaag accgaaagct
     1021 ctaccgaatc acccaccgag gagatatcgt ccctcaagtg cccttctggg acggttacca
     1081 gcactgctct ggtgaggtct ttattgactg gcccctgatc caccctcctc tctccaacgt
     1141 tgtcatgtgc cagggccaga gcaataaaca gtgctctgcc ggtaacactc tgctccagca
     1201 ggtcaatgtg attggaaacc atctgcagta cttcgtcacc gagggtgtct gtggtatcta
     1261 agctatttat cactctttac aacttctacc tcaactatct actttaataa atgaatatcg
     1321 tttattctct atgattactg tatatgcgtt cctctaagac aaatcgaaac cagcatgcga
     1381 tcgaatggca tacaaaagtt tcttccgaag ttgatcaatg tcctgatagt caggcagctt
     1441 gagaagattg acacaggtgg aggccgtagg gaaccgatca acctgtctac cagcgttacg
     1501 aatggcaaat gacgggttca aagccttgaa tccttgcaat ggtgccttgg atactgatgt
     1561 cacaaactta agaagcagcc gcttgtcctc ttcctcgaaa ctctcaaaca cagtccagaa
     1621 gtcctttata gtttgatctg tatccagata gcctccgtaa ttggtgtgtg tcttcaaatc
     1681 ccagacgtcc acattggcat gtcctccact gataagcatt tgaagttcat ctgcgttgaa
     1741 cattgagacc cacgaagggt caatgagctg gtatagaccg cccaagaatg catctgagaa
     1801 cagctgaagc ttcgtacgat aacttcgtat aatgtatgct atacgaagtt atagagaccg
     1861 ggttggcggc gcatttgtgt cccaaaaaac agccccaatt gccccaattg accccaaatt
     1921 gacccagtag cgggcccaac cccggcgaga gcccccttct ccccacatat caaacctccc
     1981 ccggttccca cacttgccgt taagggcgta gggtactgca gtctggaatc tacgcttgtt
     2041 cagactttgt actagtttct ttgtctggcc atccgggtaa cccatgccgg acgcaaaata
     2101 gactactgaa aatttttttg ctttgtggtt gggactttag ccaagggtat aaaagaccac
     2161 cgtccccgaa ttacctttcc tcttcttttc tctctctcct tgtcaactca cacccgaaat
     2221 cgttaagcat ttccttctga gtataagaat cattcaaaat ggtgagtttc agaggcagca
     2281 gcaattgcca cgggctttga gcacacggcc gggtgtggtc ccattcccat cgacacaaga
     2341 cgccacgtca tccgaccagc actttttgca gtactaaccg cagggtacta ctttggatga
     2401 tactgcttac agatacagaa cttctgttcc aggtgatgct gaagctattg aagctttgga
     2461 tggttctttc actactgaca ctgttttcag agttactgct actggtgatg gtttcacttt
     2521 gagagaagtt ccagttgatc caccattgac taaggttttt ccagatgatg aatccgatga
     2581 cgaatctgat gatggtgaag atggtgatcc agattctaga acttttgttg cttatggtga
     2641 tgacggtgac ttggctggtt ttgttgttgt ttcttattct ggttggaata gaagattgac
     2701 cgtcgaagat attgaagttg ctccagaaca tagaggtcat ggtgttggta gagctttgat
     2761 gggtttggct actgaatttg ctagagaaag aggtgctggt catttgtggt tggaagttac
     2821 taatgttaac gctccagcta ttcatgccta tagaagaatg ggttttacct tgtgtggttt
     2881 ggatactgca ttatacgatg gtactgcatc agatggtgaa caagccttgt atatgtctat
     2941 gccatgtcca tgatcatgta attagttatg tcacgcttac attcacgccc tccccccaca
     3001 tccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt
     3061 tttatagtta tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg
     3121 tacagacgcg tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga
     3181 cgctcgataa cttcgtataa tgtatgctat acgaagttat gtgatatcag atccactagt
     3241 ggcctggggc tggcgtgtga aggagcatgt gcaaagggcc gcgcagactt gtggagtgct
     3301 gccgtacgag tatcggtgta cgacaaggtg gtttgtacaa ggtggtttgt accgtcggac
     3361 atgatcatct gtaccttggc gtactgtact tgtacaacca cttggtgatc agatataacc
     3421 taacagacta atcgaccgag gcattcatac tcacccgaac cagcctaaac gagataaccc
     3481 ccacagttgg ccgttaggtt agagtcttgt tcggttcttg ggttcttcag cttttgtttt
     3541 tccgctgccc gtcattgtct tctctttctt tgtgctccaa acctttcgcc agggcctgga
     3601 cggatgccgt attcaaacag gttcatgtat ggaaatgact ttggatacgg ccttggatac
     3661 gaccttggat acgaccttgg atacgacctt ggatacgacg tcggctgcga ttacgtgcag
     3721 agggagagct acatgttcca tgtgcgatgc cgtgtgagag gtctgatcgc gtgcattcgc
     3781 ggccgcattt aaatccccac ttcagaagtt cctatacttt ctagagaata ggaacttcac
     3841 tatagagtcg aataagggcg acaccccata attagcccgg gcgaaaggcc cagtctttcg
     3901 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcatg gggagtcccc
     3961 acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc aggtgggacc
     4021 accgcgctac tgccgccagg caaacaaggg gtgttatgag ccatattcag gtataaatgg
     4081 gctcgcgata atgttcagaa ttggttaatt ggttgtaaca ctgaccccta tttgtttatt
     4141 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4201 ataatattga aaaaggaaga atatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4261 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4321 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4381 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4441 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4501 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4561 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4621 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4681 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4741 cgacgagcgt gacaccacga tgcctgtagc gatggcaaca acgttgcgca aactattaac
     4801 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4861 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4921 cggagccggt gagcgtggtt ctcgcggtat catcgcagcg ctggggccag atggtaagcc
     4981 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5041 acagatcgct gagataggtg cctcactgat taagcattgg taaaagcaga gcattacgct
     5101 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgcgcgt
     5161 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5221 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5281 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5341 taccaaatac tgttcttcta gtgtagccgt agttagccca ccacttcaag aactctgtag
     5401 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5461 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5521 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5581 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5641 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5701 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5761 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5821 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5881 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     5941 ccgagcgcag cgagtcagtg agcgaggaag cggaaggcga gagtagggaa ctgccaggca
     6001 tcaaactaag cagaaggccc ctgacggatg gcctttttgc gtttctacaa actctttctg
     6061 tgttgtaaaa cgacggccag tcttaagctc gggccccctg ggcggttctg ataacgagta
     6121 atcgttaatc cgcaaataac gtaaaaaccc gcttcggcgg gtttttttat ggggggagtt
     6181 tagggaaaga gcatttgtca gaatatttaa gggcgcctgt cactttgctt gatatatgag
     6241 aattatttaa ccttataaat gagaaaaaag caacgcactt taaataagat acgttgcttt
     6301 ttcgattgat gaacacctat aattaaacta ttcatctatt atttatgatt ttttgtatat
     6361 acaatatttc tagtttgtta aagagaatta agaaaataaa tctcgaaaat aataaaggga
     6421 aaatcagttt ttgatatcaa aattatacat gtcaacgata atacaaaata taatacaaac
     6481 tataagatgt tatcagtatt tattatgcat ttagaataaa ttttgtgtcg cccttattcg
     6541 actcactata gaagttccta ttctctagaa agtataggaa cttcacttca ttttatttaa
     6601 atttgcggcc gc
//
