LOCUS       Exported                6601 bp ds-DNA     linear   SYN 20-DEC-2017
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCfB4782 (pIntC-2-Nat-TPex20-TLip2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2017 from SnapGene 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    14..513
                     /label=IntC_2 UP
     terminator      complement(527..839)
                     /label=TPex20
     misc_feature    840..868
                     /label=USER cloning site
     terminator      869..1805
                     /label=T lip2
     misc_feature    1828..1861
                     /label=loxP
     promoter        1862..2392
                     /label=PrTefintron
     CDS             2393..2962
                     /label=Nat
                     /label=Nat codon optimized
     terminator      2963..3195
                     /label=Tcyc
     misc_feature    3196..3229
                     /label=loxP
     misc_feature    3255..3754
                     /label=IntC_2 DW
     misc_feature    4212..5072
                     /label=AmpR
     misc_feature    complement(5173..5816)
                     /label=pUC ORI
ORIGIN
        1 gaatgcgtgc gataattcga cgacctgaac actcggcgac tcattgaaat accggttggt
       61 gtttctggac aggtgtcaat ggacgattcg tggagaaagc tcacttgtgg agatgggtat
      121 ctggagacac cgaaagagat caacttgtag gtattgtttt gaatgcttct ctgggtggtt
      181 gcgcacccgc tctatccttc gaacacaggc cccagggtga tgtgggatat ggtcaatgag
      241 atgtttgcca acaagtacca acttcgtgaa gacgaagatc caccttccac cttggtgatg
      301 ggccagagac gcgaaggacg acatccaaca acaacaacaa caacaacaac aacaacaacc
      361 gcagcagcag aaacgatacg aggactgcgc ttctgaggct ggaaacagca gtgttcagct
      421 tatggtcaac gagaaacaca atctcgctcc ggactgctac aagccgtcta agactacaca
      481 aagctaacag acaaatgtag gtcttagatg gaaacgtgca acgcttacgc aactaacatg
      541 aatgaatacg atatacatca aagactatga tacgcagtat tgcacactgt acgagtaagg
      601 gcactagcca ctgcactcaa gtgaaaccgt tgcccgggta cgagtatgag tatgtacagt
      661 atgtttagta ttgtacttgg acaatgcttg tatcgtacat tctcaagtgt caaacataaa
      721 tatccgttgc tatatcctcg caccaccacg tagctcgcta tatccctgtg ttgaatccat
      781 ccatcttgga ttgccaattg tgcacacaga accgggcact cacttcccca tccacacttc
      841 aacggaatgc gtgcgatcgc gtgcattcct tctgttcgga atcaacctca aggttaacgg
      901 ccacgatccc ctcgttgtta ctcttggtca gcccattgtc ggtaacgctg gctttgctaa
      961 ctgggtcgat aaactcttct ttggccagga gaaccccgat gtctccaagg tgtccaaaga
     1021 ccgaaagctc taccgaatca cccaccgagg agatatcgtc cctcaagtgc ccttctggga
     1081 cggttaccag cactgctctg gtgaggtctt tattgactgg cccctgatcc accctcctct
     1141 ctccaacgtt gtcatgtgcc agggccagag caataaacag tgctctgccg gtaacactct
     1201 gctccagcag gtcaatgtga ttggaaacca tctgcagtac ttcgtcaccg agggtgtctg
     1261 tggtatctaa gctatttatc actctttaca acttctacct caactatcta ctttaataaa
     1321 tgaatatcgt ttattctcta tgattactgt atatgcgttc ctctaagaca aatcgaaacc
     1381 agcatgcgat cgaatggcat acaaaagttt cttccgaagt tgatcaatgt cctgatagtc
     1441 aggcagcttg agaagattga cacaggtgga ggccgtaggg aaccgatcaa cctgtctacc
     1501 agcgttacga atggcaaatg acgggttcaa agccttgaat ccttgcaatg gtgccttgga
     1561 tactgatgtc acaaacttaa gaagcagccg cttgtcctct tcctcgaaac tctcaaacac
     1621 agtccagaag tcctttatag tttgatctgt atccagatag cctccgtaat tggtgtgtgt
     1681 cttcaaatcc cagacgtcca cattggcatg tcctccactg ataagcattt gaagttcatc
     1741 tgcgttgaac attgagaccc acgaagggtc aatgagctgg tatagaccgc ccaagaatgc
     1801 atctgagaac agctgaagct tcgtacgata acttcgtata atgtatgcta tacgaagtta
     1861 tagagaccgg gttggcggcg catttgtgtc ccaaaaaaca gccccaattg ccccaattga
     1921 ccccaaattg acccagtagc gggcccaacc ccggcgagag cccccttctc cccacatatc
     1981 aaacctcccc cggttcccac acttgccgtt aagggcgtag ggtactgcag tctggaatct
     2041 acgcttgttc agactttgta ctagtttctt tgtctggcca tccgggtaac ccatgccgga
     2101 cgcaaaatag actactgaaa atttttttgc tttgtggttg ggactttagc caagggtata
     2161 aaagaccacc gtccccgaat tacctttcct cttcttttct ctctctcctt gtcaactcac
     2221 acccgaaatc gttaagcatt tccttctgag tataagaatc attcaaaatg gtgagtttca
     2281 gaggcagcag caattgccac gggctttgag cacacggccg ggtgtggtcc cattcccatc
     2341 gacacaagac gccacgtcat ccgaccagca ctttttgcag tactaaccgc agggtactac
     2401 tttggatgat actgcttaca gatacagaac ttctgttcca ggtgatgctg aagctattga
     2461 agctttggat ggttctttca ctactgacac tgttttcaga gttactgcta ctggtgatgg
     2521 tttcactttg agagaagttc cagttgatcc accattgact aaggtttttc cagatgatga
     2581 atccgatgac gaatctgatg atggtgaaga tggtgatcca gattctagaa cttttgttgc
     2641 ttatggtgat gacggtgact tggctggttt tgttgttgtt tcttattctg gttggaatag
     2701 aagattgacc gtcgaagata ttgaagttgc tccagaacat agaggtcatg gtgttggtag
     2761 agctttgatg ggtttggcta ctgaatttgc tagagaaaga ggtgctggtc atttgtggtt
     2821 ggaagttact aatgttaacg ctccagctat tcatgcctat agaagaatgg gttttacctt
     2881 gtgtggtttg gatactgcat tatacgatgg tactgcatca gatggtgaac aagccttgta
     2941 tatgtctatg ccatgtccat gatcatgtaa ttagttatgt cacgcttaca ttcacgccct
     3001 ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct
     3061 atttattttt ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt
     3121 ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag
     3181 gttttgggac gctcgataac ttcgtataat gtatgctata cgaagttatg tgatatcaga
     3241 tccactagtg gcctagaact cctcggatac ctccgagtgt gcagcccaca attatagggt
     3301 cgagactgaa gtcaggatgc agtcgtcgag atcgccgaat tgagcagtgt caggaacctg
     3361 aacagctgaa tgaagatgcc gaaatgagag ctagaattaa gccgaaagag ctggaactgg
     3421 aactggagct ggaattggag agtgacgatt ccctacaagt tggcaatggt actggcccag
     3481 tcattctctg tctatttcat cgaagagcat ctgtggggtg agtactaccc tgtagtacat
     3541 tgtgctgtgt ccttgtggat tagtgccact ctagaggcta tatagacagc aatggtatac
     3601 cattcgcaca gcagtttcac tgtatagtac accatgggac tctgatcctc tctaccgagg
     3661 tctggttggt tttgacaggc gtttttatca ggcgttttta tcaggcgttt ttatcaggcg
     3721 tttttatcag gcgtttttgt caggcgtttc aatcatcgcg tgcattcgcg gccgcattta
     3781 aatccccact tcagaagttc ctatactttc tagagaatag gaacttcact atagagtcga
     3841 ataagggcga caccccataa ttagcccggg cgaaaggccc agtctttcga ctgagccttt
     3901 cgttttattt gatgcctggc agttccctac tctcgcatgg ggagtcccca cactaccatc
     3961 ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact
     4021 gccgccaggc aaacaagggg tgttatgagc catattcagg tataaatggg ctcgcgataa
     4081 tgttcagaat tggttaattg gttgtaacac tgacccctat ttgtttattt ttctaaatac
     4141 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4201 aaaggaagaa tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4261 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4321 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4381 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4441 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4501 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4561 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4621 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4681 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4741 acaccacgat gcctgtagcg atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4801 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4861 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatcc ggagccggtg
     4921 agcgtggttc tcgcggtatc atcgcagcgc tggggccaga tggtaagccc tcccgtatcg
     4981 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5041 agataggtgc ctcactgatt aagcattggt aaaagcagag cattacgctg acttgacggg
     5101 acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgcgcgtc gttccactga
     5161 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5221 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5281 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5341 gttcttctag tgtagccgta gttagcccac cacttcaaga actctgtagc accgcctaca
     5401 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5461 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5521 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5581 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5641 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5701 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5761 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5821 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5881 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     5941 gagtcagtga gcgaggaagc ggaaggcgag agtagggaac tgccaggcat caaactaagc
     6001 agaaggcccc tgacggatgg cctttttgcg tttctacaaa ctctttctgt gttgtaaaac
     6061 gacggccagt cttaagctcg ggccccctgg gcggttctga taacgagtaa tcgttaatcc
     6121 gcaaataacg taaaaacccg cttcggcggg tttttttatg gggggagttt agggaaagag
     6181 catttgtcag aatatttaag ggcgcctgtc actttgcttg atatatgaga attatttaac
     6241 cttataaatg agaaaaaagc aacgcacttt aaataagata cgttgctttt tcgattgatg
     6301 aacacctata attaaactat tcatctatta tttatgattt tttgtatata caatatttct
     6361 agtttgttaa agagaattaa gaaaataaat ctcgaaaata ataaagggaa aatcagtttt
     6421 tgatatcaaa attatacatg tcaacgataa tacaaaatat aatacaaact ataagatgtt
     6481 atcagtattt attatgcatt tagaataaat tttgtgtcgc ccttattcga ctcactatag
     6541 aagttcctat tctctagaaa gtataggaac ttcacttcat tttatttaaa tttgcggccg
     6601 c
//
