LOCUS       Exported                6563 bp ds-DNA     linear   SYN 20-DEC-2017
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCfB4786 (pIntC-3-Nat-TPex20-TLip2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2017 from SnapGene 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    14..515
                     /label=IntC_3 UP
     terminator      complement(529..841)
                     /label=TPex20
     misc_feature    842..870
                     /label=USER cloning site
     terminator      871..1807
                     /label=T lip2
     misc_feature    1830..1863
                     /label=loxP
     promoter        1864..2394
                     /label=PrTefintron
     CDS             2395..2964
                     /label=Nat
                     /label=Nat codon optimized
     terminator      2965..3197
                     /label=Tcyc
     misc_feature    3198..3231
                     /label=loxP
     misc_feature    3257..3716
                     /label=IntC_3 DW
     misc_feature    4174..5034
                     /label=AmpR
     misc_feature    complement(5135..5778)
                     /label=pUC ORI
ORIGIN
        1 gaatgcgtgc gataaaaaac tgtagtagtg tggtgatgga gtcataaccc gctccggcta
       61 tgtgcggata cagtattgtc tcagacagtg gtcgcgagca atgtcttgtg catcttcata
      121 cttttttttt ttttattttg agtggggagg ttgaggttgg gtcagaggtt gggtcatcca
      181 tgaagaggtt aacagaacga gccacgatag ggggaaggat cttcaactcg atccgctatt
      241 gttctcagtg atggcaaata gattcatcta agacctaccg gtacaagtaa agtacagcac
      301 acgtaatttt tcttgcggct agtctactct cagtgcatgt aacgtgcgga tctagcccat
      361 gaacaggtgg tttttaccct cctgctctcc acatttccag atagatgcag gcttctatgg
      421 ttgttccaca ctcttgtcag aggagatgcc cgtatacgac caccataagc gaccctatta
      481 cggggcccga acaagccccg ttgatagaca ctgtaacgtg caacgcttac gcaactaaca
      541 tgaatgaata cgatatacat caaagactat gatacgcagt attgcacact gtacgagtaa
      601 gggcactagc cactgcactc aagtgaaacc gttgcccggg tacgagtatg agtatgtaca
      661 gtatgtttag tattgtactt ggacaatgct tgtatcgtac attctcaagt gtcaaacata
      721 aatatccgtt gctatatcct cgcaccacca cgtagctcgc tatatccctg tgttgaatcc
      781 atccatcttg gattgccaat tgtgcacaca gaaccgggca ctcacttccc catccacact
      841 tcaacggaat gcgtgcgatc gcgtgcattc cttctgttcg gaatcaacct caaggttaac
      901 ggccacgatc ccctcgttgt tactcttggt cagcccattg tcggtaacgc tggctttgct
      961 aactgggtcg ataaactctt ctttggccag gagaaccccg atgtctccaa ggtgtccaaa
     1021 gaccgaaagc tctaccgaat cacccaccga ggagatatcg tccctcaagt gcccttctgg
     1081 gacggttacc agcactgctc tggtgaggtc tttattgact ggcccctgat ccaccctcct
     1141 ctctccaacg ttgtcatgtg ccagggccag agcaataaac agtgctctgc cggtaacact
     1201 ctgctccagc aggtcaatgt gattggaaac catctgcagt acttcgtcac cgagggtgtc
     1261 tgtggtatct aagctattta tcactcttta caacttctac ctcaactatc tactttaata
     1321 aatgaatatc gtttattctc tatgattact gtatatgcgt tcctctaaga caaatcgaaa
     1381 ccagcatgcg atcgaatggc atacaaaagt ttcttccgaa gttgatcaat gtcctgatag
     1441 tcaggcagct tgagaagatt gacacaggtg gaggccgtag ggaaccgatc aacctgtcta
     1501 ccagcgttac gaatggcaaa tgacgggttc aaagccttga atccttgcaa tggtgccttg
     1561 gatactgatg tcacaaactt aagaagcagc cgcttgtcct cttcctcgaa actctcaaac
     1621 acagtccaga agtcctttat agtttgatct gtatccagat agcctccgta attggtgtgt
     1681 gtcttcaaat cccagacgtc cacattggca tgtcctccac tgataagcat ttgaagttca
     1741 tctgcgttga acattgagac ccacgaaggg tcaatgagct ggtatagacc gcccaagaat
     1801 gcatctgaga acagctgaag cttcgtacga taacttcgta taatgtatgc tatacgaagt
     1861 tatagagacc gggttggcgg cgcatttgtg tcccaaaaaa cagccccaat tgccccaatt
     1921 gaccccaaat tgacccagta gcgggcccaa ccccggcgag agcccccttc tccccacata
     1981 tcaaacctcc cccggttccc acacttgccg ttaagggcgt agggtactgc agtctggaat
     2041 ctacgcttgt tcagactttg tactagtttc tttgtctggc catccgggta acccatgccg
     2101 gacgcaaaat agactactga aaattttttt gctttgtggt tgggacttta gccaagggta
     2161 taaaagacca ccgtccccga attacctttc ctcttctttt ctctctctcc ttgtcaactc
     2221 acacccgaaa tcgttaagca tttccttctg agtataagaa tcattcaaaa tggtgagttt
     2281 cagaggcagc agcaattgcc acgggctttg agcacacggc cgggtgtggt cccattccca
     2341 tcgacacaag acgccacgtc atccgaccag cactttttgc agtactaacc gcagggtact
     2401 actttggatg atactgctta cagatacaga acttctgttc caggtgatgc tgaagctatt
     2461 gaagctttgg atggttcttt cactactgac actgttttca gagttactgc tactggtgat
     2521 ggtttcactt tgagagaagt tccagttgat ccaccattga ctaaggtttt tccagatgat
     2581 gaatccgatg acgaatctga tgatggtgaa gatggtgatc cagattctag aacttttgtt
     2641 gcttatggtg atgacggtga cttggctggt tttgttgttg tttcttattc tggttggaat
     2701 agaagattga ccgtcgaaga tattgaagtt gctccagaac atagaggtca tggtgttggt
     2761 agagctttga tgggtttggc tactgaattt gctagagaaa gaggtgctgg tcatttgtgg
     2821 ttggaagtta ctaatgttaa cgctccagct attcatgcct atagaagaat gggttttacc
     2881 ttgtgtggtt tggatactgc attatacgat ggtactgcat cagatggtga acaagccttg
     2941 tatatgtcta tgccatgtcc atgatcatgt aattagttat gtcacgctta cattcacgcc
     3001 ctccccccac atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc
     3061 ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc
     3121 ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga
     3181 aggttttggg acgctcgata acttcgtata atgtatgcta tacgaagtta tgtgatatca
     3241 gatccactag tggcctgcca tagcactatt gtagagtggc cattgttcgt gtcgatccgt
     3301 acgacgatca tggagcgagc ggccacgaaa ttagatagcc actaaaacta gtcctgtcca
     3361 cggaaggccg tttgttagcc gcttatatcg ttggaacaat acttttgttg ccggcatcta
     3421 atttcataat ttcgtgatta atttttcccg ccaaaaaata aaaaatcaaa aacaaaaaat
     3481 aaaaaggaac ttcggtaaga gcgctcccga aaccggaaat gcatggtttc tctcatgtgt
     3541 gcctcttgct tgtctcaggc cggtccagaa acaaacctcg agcttccgcc tccaagaccc
     3601 tgctttcgga gaagcagcca atggctgttg tttgtcactg ttccctgtct tggttttttt
     3661 ggcccttctt ttcttcttcc ccccatgaaa ccttgtgtgt gtcctgtgca ataattatcg
     3721 cgtgcattcg cggccgcatt taaatcccca cttcagaagt tcctatactt tctagagaat
     3781 aggaacttca ctatagagtc gaataagggc gacaccccat aattagcccg ggcgaaaggc
     3841 ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcat
     3901 ggggagtccc cacactacca tcggcgctac ggcgtttcac ttctgagttc ggcatggggt
     3961 caggtgggac caccgcgcta ctgccgccag gcaaacaagg ggtgttatga gccatattca
     4021 ggtataaatg ggctcgcgat aatgttcaga attggttaat tggttgtaac actgacccct
     4081 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4141 taaatgcttc aataatattg aaaaaggaag aatatgagta ttcaacattt ccgtgtcgcc
     4201 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4261 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4321 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4381 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4441 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4501 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4561 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4621 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4681 gccataccaa acgacgagcg tgacaccacg atgcctgtag cgatggcaac aacgttgcgc
     4741 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4801 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4861 gctgataaat ccggagccgg tgagcgtggt tctcgcggta tcatcgcagc gctggggcca
     4921 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4981 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaaaagcag
     5041 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     5101 tacgcgcgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5161 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5221 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5281 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttagccc accacttcaa
     5341 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5401 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5461 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5521 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5581 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5641 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5701 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5761 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5821 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     5881 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga
     5941 actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca
     6001 aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct
     6061 gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta
     6121 tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct
     6181 tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga
     6241 tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat
     6301 tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa
     6361 taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat
     6421 ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc
     6481 gcccttattc gactcactat agaagttcct attctctaga aagtatagga acttcacttc
     6541 attttattta aatttgcggc cgc
//
