LOCUS       Exported                6605 bp ds-DNA     linear   SYN 20-DEC-2017
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCfB4779 (pIntE-2-Nat-TPex20-TLip2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2017 from SnapGene 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    14..513
                     /label=IntE-2 UP
     terminator      complement(527..839)
                     /label=TPex20
     misc_feature    840..868
                     /label=USER cloning site
     terminator      869..1805
                     /label=T lip2
     misc_feature    1828..1861
                     /label=loxP
     promoter        1862..2392
                     /label=PrTefintron
     CDS             2393..2962
                     /label=Nat
                     /label=Nat codon optimized
     terminator      2963..3195
                     /label=Tcyc
     misc_feature    3196..3229
                     /label=loxP
     misc_feature    3255..3758
                     /label=IntE-2 DW
     misc_feature    4216..5076
                     /label=AmpR
     misc_feature    complement(5177..5820)
                     /label=pUC ORI
ORIGIN
        1 gaatgcgtgc gatatggtgc caaatcgctt gtcactttga cacaatgaag atattcgatt
       61 cagtcgatac catggccgtc aactttcttg tccgacctct caactttgta tctatgtctc
      121 aggacggagt gttggtccat ccagccccta ccagttccgt ggtgatgttg aaagggctat
      181 ttttatccag atcagagggt cactggagct tgaataggat ccacaagaag ttcaaaggac
      241 tttactggtg tttcttttgt ccgagaactt caacaactcc gtctggtgtc tccaagatag
      301 attctggtgc tctccacgcc tatacatctc caagtactgt acaccacaaa caccttccgg
      361 gtggttgctt acagtttcaa taggcgaccg cttgatttcg gcaaatctac acctgccagc
      421 tacgcatatt gcaggaagca gcatcatgga atggactgta tagccataag acaagaatgc
      481 tctggagaaa tcgtccgatt ttgctgcact aagacgtgca acgcttacgc aactaacatg
      541 aatgaatacg atatacatca aagactatga tacgcagtat tgcacactgt acgagtaagg
      601 gcactagcca ctgcactcaa gtgaaaccgt tgcccgggta cgagtatgag tatgtacagt
      661 atgtttagta ttgtacttgg acaatgcttg tatcgtacat tctcaagtgt caaacataaa
      721 tatccgttgc tatatcctcg caccaccacg tagctcgcta tatccctgtg ttgaatccat
      781 ccatcttgga ttgccaattg tgcacacaga accgggcact cacttcccca tccacacttc
      841 aacggaatgc gtgcgatcgc gtgcattcct tctgttcgga atcaacctca aggttaacgg
      901 ccacgatccc ctcgttgtta ctcttggtca gcccattgtc ggtaacgctg gctttgctaa
      961 ctgggtcgat aaactcttct ttggccagga gaaccccgat gtctccaagg tgtccaaaga
     1021 ccgaaagctc taccgaatca cccaccgagg agatatcgtc cctcaagtgc ccttctggga
     1081 cggttaccag cactgctctg gtgaggtctt tattgactgg cccctgatcc accctcctct
     1141 ctccaacgtt gtcatgtgcc agggccagag caataaacag tgctctgccg gtaacactct
     1201 gctccagcag gtcaatgtga ttggaaacca tctgcagtac ttcgtcaccg agggtgtctg
     1261 tggtatctaa gctatttatc actctttaca acttctacct caactatcta ctttaataaa
     1321 tgaatatcgt ttattctcta tgattactgt atatgcgttc ctctaagaca aatcgaaacc
     1381 agcatgcgat cgaatggcat acaaaagttt cttccgaagt tgatcaatgt cctgatagtc
     1441 aggcagcttg agaagattga cacaggtgga ggccgtaggg aaccgatcaa cctgtctacc
     1501 agcgttacga atggcaaatg acgggttcaa agccttgaat ccttgcaatg gtgccttgga
     1561 tactgatgtc acaaacttaa gaagcagccg cttgtcctct tcctcgaaac tctcaaacac
     1621 agtccagaag tcctttatag tttgatctgt atccagatag cctccgtaat tggtgtgtgt
     1681 cttcaaatcc cagacgtcca cattggcatg tcctccactg ataagcattt gaagttcatc
     1741 tgcgttgaac attgagaccc acgaagggtc aatgagctgg tatagaccgc ccaagaatgc
     1801 atctgagaac agctgaagct tcgtacgata acttcgtata atgtatgcta tacgaagtta
     1861 tagagaccgg gttggcggcg catttgtgtc ccaaaaaaca gccccaattg ccccaattga
     1921 ccccaaattg acccagtagc gggcccaacc ccggcgagag cccccttctc cccacatatc
     1981 aaacctcccc cggttcccac acttgccgtt aagggcgtag ggtactgcag tctggaatct
     2041 acgcttgttc agactttgta ctagtttctt tgtctggcca tccgggtaac ccatgccgga
     2101 cgcaaaatag actactgaaa atttttttgc tttgtggttg ggactttagc caagggtata
     2161 aaagaccacc gtccccgaat tacctttcct cttcttttct ctctctcctt gtcaactcac
     2221 acccgaaatc gttaagcatt tccttctgag tataagaatc attcaaaatg gtgagtttca
     2281 gaggcagcag caattgccac gggctttgag cacacggccg ggtgtggtcc cattcccatc
     2341 gacacaagac gccacgtcat ccgaccagca ctttttgcag tactaaccgc agggtactac
     2401 tttggatgat actgcttaca gatacagaac ttctgttcca ggtgatgctg aagctattga
     2461 agctttggat ggttctttca ctactgacac tgttttcaga gttactgcta ctggtgatgg
     2521 tttcactttg agagaagttc cagttgatcc accattgact aaggtttttc cagatgatga
     2581 atccgatgac gaatctgatg atggtgaaga tggtgatcca gattctagaa cttttgttgc
     2641 ttatggtgat gacggtgact tggctggttt tgttgttgtt tcttattctg gttggaatag
     2701 aagattgacc gtcgaagata ttgaagttgc tccagaacat agaggtcatg gtgttggtag
     2761 agctttgatg ggtttggcta ctgaatttgc tagagaaaga ggtgctggtc atttgtggtt
     2821 ggaagttact aatgttaacg ctccagctat tcatgcctat agaagaatgg gttttacctt
     2881 gtgtggtttg gatactgcat tatacgatgg tactgcatca gatggtgaac aagccttgta
     2941 tatgtctatg ccatgtccat gatcatgtaa ttagttatgt cacgcttaca ttcacgccct
     3001 ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct
     3061 atttattttt ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt
     3121 ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag
     3181 gttttgggac gctcgataac ttcgtataat gtatgctata cgaagttatg tgatatcaga
     3241 tccactagtg gcctgtgtgg attgcgatat tgaagtgttg actcgaagag acagctgcct
     3301 gtcaagttga ttgttagctc cttggcatac agatgcaaga aatgagagtg gccatacggc
     3361 ggcagttagc ataagatact acttgtagga gagagcgctc accaccagct aaatttagga
     3421 ccctggctgt ctatttacat ttttgcaccc tgttggctgc ctagctatgc ggccagtcag
     3481 cccgctggac atgtgtttgg catgtgtttg gcatgtgttt ggtgcaggga gaaccgcgat
     3541 ggtgaccgtc caagcaatta cccgtttgga ccagaagagt aaagagcgac atttgcaaaa
     3601 gaaacgcgct gaaaaagcaa ctccagcgat gaggaactga gagtctgaag tctctggacg
     3661 aggagtggga ggggggatgc cttctgtggg tgccgtacgt gtctaccgag aagttaacaa
     3721 ccctcctgtt ggtatagtct acaaacctgg cgtatttcat cgcgtgcatt cgcggccgca
     3781 tttaaatccc cacttcagaa gttcctatac tttctagaga ataggaactt cactatagag
     3841 tcgaataagg gcgacacccc ataattagcc cgggcgaaag gcccagtctt tcgactgagc
     3901 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc atggggagtc cccacactac
     3961 catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc
     4021 tactgccgcc aggcaaacaa ggggtgttat gagccatatt caggtataaa tgggctcgcg
     4081 ataatgttca gaattggtta attggttgta acactgaccc ctatttgttt atttttctaa
     4141 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4201 tgaaaaagga agaatatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4261 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4321 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4381 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4441 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4501 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4561 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4621 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4681 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4741 cgtgacacca cgatgcctgt agcgatggca acaacgttgc gcaaactatt aactggcgaa
     4801 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4861 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atccggagcc
     4921 ggtgagcgtg gttctcgcgg tatcatcgca gcgctggggc cagatggtaa gccctcccgt
     4981 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5041 gctgagatag gtgcctcact gattaagcat tggtaaaagc agagcattac gctgacttga
     5101 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgcg cgtcgttcca
     5161 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5221 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5281 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5341 tactgttctt ctagtgtagc cgtagttagc ccaccacttc aagaactctg tagcaccgcc
     5401 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5461 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5521 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5581 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5641 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5701 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5761 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5821 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5881 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5941 cagcgagtca gtgagcgagg aagcggaagg cgagagtagg gaactgccag gcatcaaact
     6001 aagcagaagg cccctgacgg atggcctttt tgcgtttcta caaactcttt ctgtgttgta
     6061 aaacgacggc cagtcttaag ctcgggcccc ctgggcggtt ctgataacga gtaatcgtta
     6121 atccgcaaat aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga gtttagggaa
     6181 agagcatttg tcagaatatt taagggcgcc tgtcactttg cttgatatat gagaattatt
     6241 taaccttata aatgagaaaa aagcaacgca ctttaaataa gatacgttgc tttttcgatt
     6301 gatgaacacc tataattaaa ctattcatct attatttatg attttttgta tatacaatat
     6361 ttctagtttg ttaaagagaa ttaagaaaat aaatctcgaa aataataaag ggaaaatcag
     6421 tttttgatat caaaattata catgtcaacg ataatacaaa atataataca aactataaga
     6481 tgttatcagt atttattatg catttagaat aaattttgtg tcgcccttat tcgactcact
     6541 atagaagttc ctattctcta gaaagtatag gaacttcact tcattttatt taaatttgcg
     6601 gccgc
//
