LOCUS       Exported                5152 bp ds-DNA     linear   SYN 20-DEC-2017
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCfB6681 (pIntE_3-TPex20-TLip2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2017 from SnapGene 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    14..536
                     /label=IntE_3 UP
     terminator      complement(550..862)
                     /label=TPex20
     misc_feature    863..891
                     /label=USER cloning site
     terminator      892..1828
                     /label=T lip2
     misc_feature    1837..2305
                     /label=IntE-3 DW
     misc_feature    2763..3623
                     /label=AmpR
     misc_feature    complement(3724..4367)
                     /label=pUC ORI
ORIGIN
        1 gaatgcgtgc gattaacttt tttcgtgact ctgttctcca cggtcaggtg tcgtatacgt
       61 aagtactgat actcgctcgt actggtactg tgcggtacat acacagtacg ttggcaccag
      121 tgacaattgt gctatcgata ctgttataag cggcttctat ccatggttta ctgaggagaa
      181 atgttctagc agtgacgtta caagtatcgt cactacatta tgttgccatg ttcgcacaat
      241 acaacataca gacccgtttt caatgcgacg tatacgttca ggtaccttca gtgcatgtgt
      301 atgtaccaca ttatacgaaa cagctcgcac tatataccat gcaacttgta caagtatggg
      361 ccacattgtg acaccaatac agaacgcaca aacggtacac cgacgcagga agtttggaag
      421 agcgttcatg ccgattgtgt actcacagta ccagtacaat acaccggtac ttcttccctg
      481 tatcgtacga acttgtactc ctagtctaaa tgacctcaaa ctcaagacat caaacaacgt
      541 gcaacgctta cgcaactaac atgaatgaat acgatataca tcaaagacta tgatacgcag
      601 tattgcacac tgtacgagta agggcactag ccactgcact caagtgaaac cgttgcccgg
      661 gtacgagtat gagtatgtac agtatgttta gtattgtact tggacaatgc ttgtatcgta
      721 cattctcaag tgtcaaacat aaatatccgt tgctatatcc tcgcaccacc acgtagctcg
      781 ctatatccct gtgttgaatc catccatctt ggattgccaa ttgtgcacac agaaccgggc
      841 actcacttcc ccatccacac ttcaacggaa tgcgtgcgat cgcgtgcatt ccttctgttc
      901 ggaatcaacc tcaaggttaa cggccacgat cccctcgttg ttactcttgg tcagcccatt
      961 gtcggtaacg ctggctttgc taactgggtc gataaactct tctttggcca ggagaacccc
     1021 gatgtctcca aggtgtccaa agaccgaaag ctctaccgaa tcacccaccg aggagatatc
     1081 gtccctcaag tgcccttctg ggacggttac cagcactgct ctggtgaggt ctttattgac
     1141 tggcccctga tccaccctcc tctctccaac gttgtcatgt gccagggcca gagcaataaa
     1201 cagtgctctg ccggtaacac tctgctccag caggtcaatg tgattggaaa ccatctgcag
     1261 tacttcgtca ccgagggtgt ctgtggtatc taagctattt atcactcttt acaacttcta
     1321 cctcaactat ctactttaat aaatgaatat cgtttattct ctatgattac tgtatatgcg
     1381 ttcctctaag acaaatcgaa accagcatgc gatcgaatgg catacaaaag tttcttccga
     1441 agttgatcaa tgtcctgata gtcaggcagc ttgagaagat tgacacaggt ggaggccgta
     1501 gggaaccgat caacctgtct accagcgtta cgaatggcaa atgacgggtt caaagccttg
     1561 aatccttgca atggtgcctt ggatactgat gtcacaaact taagaagcag ccgcttgtcc
     1621 tcttcctcga aactctcaaa cacagtccag aagtccttta tagtttgatc tgtatccaga
     1681 tagcctccgt aattggtgtg tgtcttcaaa tcccagacgt ccacattggc atgtcctcca
     1741 ctgataagca tttgaagttc atctgcgttg aacattgaga cccacgaagg gtcaatgagc
     1801 tggtatagac cgcccaagaa tgcatctgag tggccttctg gcacagaaat gaccacacat
     1861 acccagttaa ctaagccatt ttcttcatca gcgatggaaa tgcatttaaa gatggttcta
     1921 tttcatacaa ctacccattg actaatggtt attcttgtaa aatgatggag aacttctact
     1981 acaagtacaa atactacacg cgaagttccc cagattgcac cgccacaaac accaatcgaa
     2041 ggtttctctc catacatttt tcccatttgg gacctgtgca taattataat tcatcaccat
     2101 aaaaatttcc tgttgcataa caacctgact cctgaagttt cgcgcatgtg tacatattgc
     2161 tccatcttca agtacagtac aggcacagta aggtgacacg tagtagaaca gtcaaactaa
     2221 cacatcaggc ccaccatcca ccttacacgt acggtacttg tagaaaagcg cttcgttcaa
     2281 ttcaggtttt aggcatttcc ttcacatcgc gtgcattcgc ggccgcattt aaatccccac
     2341 ttcagaagtt cctatacttt ctagagaata ggaacttcac tatagagtcg aataagggcg
     2401 acaccccata attagcccgg gcgaaaggcc cagtctttcg actgagcctt tcgttttatt
     2461 tgatgcctgg cagttcccta ctctcgcatg gggagtcccc acactaccat cggcgctacg
     2521 gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac tgccgccagg
     2581 caaacaaggg gtgttatgag ccatattcag gtataaatgg gctcgcgata atgttcagaa
     2641 ttggttaatt ggttgtaaca ctgaccccta tttgtttatt tttctaaata cattcaaata
     2701 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2761 atatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     2821 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     2881 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     2941 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3001 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3061 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3121 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3181 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3241 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3301 tgcctgtagc gatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3361 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3421 gctcggccct tccggctggc tggtttattg ctgataaatc cggagccggt gagcgtggtt
     3481 ctcgcggtat catcgcagcg ctggggccag atggtaagcc ctcccgtatc gtagttatct
     3541 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     3601 cctcactgat taagcattgg taaaagcaga gcattacgct gacttgacgg gacggcgcaa
     3661 gctcatgacc aaaatccctt aacgtgagtt acgcgcgcgt cgttccactg agcgtcagac
     3721 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3781 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3841 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3901 gtgtagccgt agttagccca ccacttcaag aactctgtag caccgcctac atacctcgct
     3961 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4021 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4081 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4141 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4201 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4261 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4321 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4381 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4441 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4501 agcgaggaag cggaaggcga gagtagggaa ctgccaggca tcaaactaag cagaaggccc
     4561 ctgacggatg gcctttttgc gtttctacaa actctttctg tgttgtaaaa cgacggccag
     4621 tcttaagctc gggccccctg ggcggttctg ataacgagta atcgttaatc cgcaaataac
     4681 gtaaaaaccc gcttcggcgg gtttttttat ggggggagtt tagggaaaga gcatttgtca
     4741 gaatatttaa gggcgcctgt cactttgctt gatatatgag aattatttaa ccttataaat
     4801 gagaaaaaag caacgcactt taaataagat acgttgcttt ttcgattgat gaacacctat
     4861 aattaaacta ttcatctatt atttatgatt ttttgtatat acaatatttc tagtttgtta
     4921 aagagaatta agaaaataaa tctcgaaaat aataaaggga aaatcagttt ttgatatcaa
     4981 aattatacat gtcaacgata atacaaaata taatacaaac tataagatgt tatcagtatt
     5041 tattatgcat ttagaataaa ttttgtgtcg cccttattcg actcactata gaagttccta
     5101 ttctctagaa agtataggaa cttcacttca ttttatttaa atttgcggcc gc
//
