LOCUS Exported 6329 bp ds-DNA linear SYN 20-DEC-2017 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pCfB6631 (pNat-YLgRNA2_IntD_1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6329) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 20, 2017 from SnapGene 4.1.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6329 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 122..1123 /label=Pr-Exp1YL /note="Oriinal location: 122..1123" /note="Unknown feature type:eukaryotic" gene 1124..1696 /label=Nat /note="Oriinal location: 1124..1696" terminator 1697..1929 /label=Tcyc /note="Oriinal location: 1697..1929" promoter 1939..2924 /label=Pot1 promoter + 50bp /note="Oriinal location: 1939..2924" /note="Unknown feature type:eukaryotic" misc_feature 2925..3143 /label=Cen1 /note="Oriinal location: 2925..3143" promoter 3162..3371 /label=Pr-tRNA-Gly /note="Oriinal location: 3162..3371" /note="Unknown feature type:eukaryotic" misc_feature 3372..3391 /label=specific gRNA misc_feature 3392..3470 /label=tracrRNA misc_feature 3471..3490 /label=TerRPR1 misc_feature 4289..4932 /label=pUC ORI misc_feature complement(5033..5893) /label=AmpR ORIGIN 1 gcggccgcta tgtctgataa aaggatgtaa cataggcaag ctgctcgtga gtgttgagta 61 cgaaccttag atccaaatca cccgcaccca cggatatact tgcttgaata tacagtagta 121 taaggagttt ggcgcccgtt ttttcgagcc ccacacgttt cggtgagtat gagcggcggc 181 agattcgagc gtttccggtt tccgcggctg gacgagagcc catgatgggg gctcccacca 241 ccagcaatca gggccctgat tacacaccca cctgtaatgt catgctgttc atcgtggtta 301 atgctgctgt gtgctgtgtg tgtgtgttgt ttggcgctca ttgttgcgtt atgcagcgta 361 caccacaata ttggaagctt attagccttt ctattttttc gtttgcaagg cttaacaaca 421 ttgctgtgga gagggatggg gatatggagg ccgctggagg gagtcggaga ggcgttttgg 481 agcggcttgg cctggcgccc agctcgcgaa acgcacctag gaccctttgg cacgccgaaa 541 tgtgccactt ttcagtctag taacgcctta cctacgtcat tccatgcatg catgtttgcg 601 ccttttttcc cttgcccttg atcgccacac agtacagtgc actgtacagt ggaggttttg 661 ggggggtctt agatgggagc taaaagcggc ctagcggtac actagtggga ttgtatggag 721 tggcatggag cctaggtgga gcctgacagg acgcacgacc ggctagcccg tgacagacga 781 tgggtggctc ctgttgtcca ccgcgtacaa atgtttgggc caaagtcttg tcagccttgc 841 ttgcgaacct aattcccaat tttgtcactt cgcaccccca ttgatcgagc cctaacccct 901 gcccatcagg caatccaatt aagctcgcat tgtctgcctt gtttagtttg gctcctgccc 961 gtttcggcgt ccacttgcac aaacacaaac aagcattata tataaggctc gtctctccct 1021 cccaaccaca ctcacttttt tgcccgtctt cccttgctaa cacaaaagtc aagaacacaa 1081 acaaccaccc caaccccctt acacacaaga catatctaca gcaatgggta ctactttgga 1141 tgatactgct tacagataca gaacttctgt tccaggtgat gctgaagcta ttgaagcttt 1201 ggatggttct ttcactactg acactgtttt cagagttact gctactggtg atggtttcac 1261 tttgagagaa gttccagttg atccaccatt gactaaggtt tttccagatg atgaatccga 1321 tgacgaatct gatgatggtg aagatggtga tccagattct agaacttttg ttgcttatgg 1381 tgatgacggt gacttggctg gttttgttgt tgtttcttat tctggttgga atagaagatt 1441 gaccgtcgaa gatattgaag ttgctccaga acatagaggt catggtgttg gtagagcttt 1501 gatgggtttg gctactgaat ttgctagaga aagaggtgct ggtcatttgt ggttggaagt 1561 tactaatgtt aacgctccag ctattcatgc ctatagaaga atgggtttta ccttgtgtgg 1621 tttggatact gcattatacg atggtactgc atcagatggt gaacaagcct tgtatatgtc 1681 tatgccatgt ccatgatcat gtaattagtt atgtcacgct tacattcacg ccctcccccc 1741 acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat 1801 ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt 1861 ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg 1921 ggacgctcga gcaggcttgg aggcgacgtg gcagtggctc gcgggaggat cggcgggaaa 1981 ctccgggata tccgtcgaga gtttacacgt gaatgggcag cgcaatccgt tgacgacgat 2041 acgactggca aagtagcgac gatacctgcc agacaggtga catgtgcagg ccgcactaac 2101 aaggaaacgg gcgctggggg gggcgggctt ctagactttg cccttgaaca ggaatctagt 2161 gggggcttgt ctttccgcca atgggggagc gcctgttgag cgaccgtgca tgctggaacg 2221 ccaagtgtat gtacagctgg tggtctcgca gcggtatgtg acgggactta catctctcgt 2281 tttttcatga ccacgttttc acaggctcgg aggtacgtta aagttttgaa ggctgcatct 2341 gaaccgaggt atgggggagt ttgaagagca acagtgttgg ggctgagggg gccaagatcg 2401 gggcaagcag aggtcttaga tcaattgtgg ggatcccaaa gggctcgtta tcaccttttt 2461 ccacccaatt cgggtcccaa ttgatccact actggcttgc ccaagttacc ccagaaatgc 2521 cgccccggat ttctccaaaa acctaataag cttcatggaa cttggtggaa gtgactttct 2581 acagagtgga gagaaccgtg gacacgtggc aatggcgctg accgtgtccc cgagccgaat 2641 cgacgtgagg ggagaacgga gtatctgcgg tcatgtgacc ttccagagcg gcgtcgccag 2701 tgtgcacgcg gtgaccccca gtttgggtct ctgtcacacg catactacct cggctctcca 2761 catgctgaac tttatctttc gtggggatca taccgaaagt tgcaactacc aggtgtatat 2821 aaagcctggt agactccccc cactttggac ctcatccaac caagacacac aaaaatggac 2881 cgacttaaca acctcgccac ccagctcgag cagaaccccg ccaattcgta gataatggaa 2941 tacaaatgga tatccagagt atacacatgg atagtataca ctgacacgac aattctgtat 3001 ctctttatgt taactactgt gaggcattaa atagagcttg atatataaaa tgttacattt 3061 cacagtctga acttttgcag attacctaat ttggtaagat attaattatg aactgaaagt 3121 tgatggcatc cctaaatttg atgcaacgga atgcgtgcga tagtgaatca ttgctaacag 3181 atcaagtaat cgtatcaatc gttccacgtc ttatccagac tcgatttccg ccctaaccta 3241 aggaaactgc tgagtaattt cgaggttggt tcctaacaaa agccaagaaa cgcgccgatg 3301 gtttagtggt aaaatccatc gttgccatcg atgggccccc ggttcgattc cgggtcggcg 3361 caggttggtt agcagacgca gacggagact ggttttagag ctagaaatag caagttaaaa 3421 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtggtgc tttttttgtg 3481 tgggtacggt atcgcgtgca ttcgcggccg caaatttaaa taaaatgaag tgaagttcct 3541 atactttcta gagaatagga acttctatag tgagtcgaat aagggcgaca caaaatttat 3601 tctaaatgca taataaatac tgataacatc ttatagtttg tattatattt tgtattatcg 3661 ttgacatgta taattttgat atcaaaaact gattttccct ttattatttt cgagatttat 3721 tttcttaatt ctctttaaca aactagaaat attgtatata caaaaaatca taaataatag 3781 atgaatagtt taattatagg tgttcatcaa tcgaaaaagc aacgtatctt atttaaagtg 3841 cgttgctttt ttctcattta taaggttaaa taattctcat atatcaagca aagtgacagg 3901 cgcccttaaa tattctgaca aatgctcttt ccctaaactc cccccataaa aaaacccgcc 3961 gaagcgggtt tttacgttat ttgcggatta acgattactc gttatcagaa ccgcccaggg 4021 ggcccgagct taagactggc cgtcgtttta caacacagaa agagtttgta gaaacgcaaa 4081 aaggccatcc gtcaggggcc ttctgcttag tttgatgcct ggcagttccc tactctcgcc 4141 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 4201 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 4261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 4321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 4381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 4441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 4501 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 4561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 4621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 4681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtgggc 4741 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 4801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 4861 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 4921 gatcttttct acggggtctg acgctcagtg gaacgacgcg cgcgtaactc acgttaaggg 4981 attttggtca tgagcttgcg ccgtcccgtc aagtcagcgt aatgctctgc ttttaccaat 5041 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 5101 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagcgctg 5161 cgatgatacc gcgagaacca cgctcaccgg ctccggattt atcagcaata aaccagccag 5221 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 5281 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 5341 ccatcgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 5401 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 5461 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 5521 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 5581 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 5641 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 5701 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 5761 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 5821 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 5881 gttgaatact catattcttc ctttttcaat attattgaag catttatcag ggttattgtc 5941 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gtcagtgtta 6001 caaccaatta accaattctg aacattatcg cgagcccatt tatacctgaa tatggctcat 6061 aacacccctt gtttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac 6121 tcagaagtga aacgccgtag cgccgatggt agtgtgggga ctccccatgc gagagtaggg 6181 aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgcccggg 6241 ctaattatgg ggtgtcgccc ttattcgact ctatagtgaa gttcctattc tctagaaagt 6301 ataggaactt ctgaagtggg gatttaaat //