LOCUS       Exported                6329 bp ds-DNA     linear   SYN 20-DEC-2017
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCfB6631 (pNat-YLgRNA2_IntD_1)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2017 from SnapGene 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        122..1123
                     /label=Pr-Exp1YL
                     /note="Oriinal location: 122..1123"
                     /note="Unknown feature type:eukaryotic"
     gene            1124..1696
                     /label=Nat
                     /note="Oriinal location: 1124..1696"
     terminator      1697..1929
                     /label=Tcyc
                     /note="Oriinal location: 1697..1929"
     promoter        1939..2924
                     /label=Pot1 promoter + 50bp
                     /note="Oriinal location: 1939..2924"
                     /note="Unknown feature type:eukaryotic"
     misc_feature    2925..3143
                     /label=Cen1
                     /note="Oriinal location: 2925..3143"
     promoter        3162..3371
                     /label=Pr-tRNA-Gly
                     /note="Oriinal location: 3162..3371"
                     /note="Unknown feature type:eukaryotic"
     misc_feature    3372..3391
                     /label=specific gRNA
     misc_feature    3392..3470
                     /label=tracrRNA
     misc_feature    3471..3490
                     /label=TerRPR1
     misc_feature    4289..4932
                     /label=pUC ORI
     misc_feature    complement(5033..5893)
                     /label=AmpR
ORIGIN
        1 gcggccgcta tgtctgataa aaggatgtaa cataggcaag ctgctcgtga gtgttgagta
       61 cgaaccttag atccaaatca cccgcaccca cggatatact tgcttgaata tacagtagta
      121 taaggagttt ggcgcccgtt ttttcgagcc ccacacgttt cggtgagtat gagcggcggc
      181 agattcgagc gtttccggtt tccgcggctg gacgagagcc catgatgggg gctcccacca
      241 ccagcaatca gggccctgat tacacaccca cctgtaatgt catgctgttc atcgtggtta
      301 atgctgctgt gtgctgtgtg tgtgtgttgt ttggcgctca ttgttgcgtt atgcagcgta
      361 caccacaata ttggaagctt attagccttt ctattttttc gtttgcaagg cttaacaaca
      421 ttgctgtgga gagggatggg gatatggagg ccgctggagg gagtcggaga ggcgttttgg
      481 agcggcttgg cctggcgccc agctcgcgaa acgcacctag gaccctttgg cacgccgaaa
      541 tgtgccactt ttcagtctag taacgcctta cctacgtcat tccatgcatg catgtttgcg
      601 ccttttttcc cttgcccttg atcgccacac agtacagtgc actgtacagt ggaggttttg
      661 ggggggtctt agatgggagc taaaagcggc ctagcggtac actagtggga ttgtatggag
      721 tggcatggag cctaggtgga gcctgacagg acgcacgacc ggctagcccg tgacagacga
      781 tgggtggctc ctgttgtcca ccgcgtacaa atgtttgggc caaagtcttg tcagccttgc
      841 ttgcgaacct aattcccaat tttgtcactt cgcaccccca ttgatcgagc cctaacccct
      901 gcccatcagg caatccaatt aagctcgcat tgtctgcctt gtttagtttg gctcctgccc
      961 gtttcggcgt ccacttgcac aaacacaaac aagcattata tataaggctc gtctctccct
     1021 cccaaccaca ctcacttttt tgcccgtctt cccttgctaa cacaaaagtc aagaacacaa
     1081 acaaccaccc caaccccctt acacacaaga catatctaca gcaatgggta ctactttgga
     1141 tgatactgct tacagataca gaacttctgt tccaggtgat gctgaagcta ttgaagcttt
     1201 ggatggttct ttcactactg acactgtttt cagagttact gctactggtg atggtttcac
     1261 tttgagagaa gttccagttg atccaccatt gactaaggtt tttccagatg atgaatccga
     1321 tgacgaatct gatgatggtg aagatggtga tccagattct agaacttttg ttgcttatgg
     1381 tgatgacggt gacttggctg gttttgttgt tgtttcttat tctggttgga atagaagatt
     1441 gaccgtcgaa gatattgaag ttgctccaga acatagaggt catggtgttg gtagagcttt
     1501 gatgggtttg gctactgaat ttgctagaga aagaggtgct ggtcatttgt ggttggaagt
     1561 tactaatgtt aacgctccag ctattcatgc ctatagaaga atgggtttta ccttgtgtgg
     1621 tttggatact gcattatacg atggtactgc atcagatggt gaacaagcct tgtatatgtc
     1681 tatgccatgt ccatgatcat gtaattagtt atgtcacgct tacattcacg ccctcccccc
     1741 acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat
     1801 ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt
     1861 ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg
     1921 ggacgctcga gcaggcttgg aggcgacgtg gcagtggctc gcgggaggat cggcgggaaa
     1981 ctccgggata tccgtcgaga gtttacacgt gaatgggcag cgcaatccgt tgacgacgat
     2041 acgactggca aagtagcgac gatacctgcc agacaggtga catgtgcagg ccgcactaac
     2101 aaggaaacgg gcgctggggg gggcgggctt ctagactttg cccttgaaca ggaatctagt
     2161 gggggcttgt ctttccgcca atgggggagc gcctgttgag cgaccgtgca tgctggaacg
     2221 ccaagtgtat gtacagctgg tggtctcgca gcggtatgtg acgggactta catctctcgt
     2281 tttttcatga ccacgttttc acaggctcgg aggtacgtta aagttttgaa ggctgcatct
     2341 gaaccgaggt atgggggagt ttgaagagca acagtgttgg ggctgagggg gccaagatcg
     2401 gggcaagcag aggtcttaga tcaattgtgg ggatcccaaa gggctcgtta tcaccttttt
     2461 ccacccaatt cgggtcccaa ttgatccact actggcttgc ccaagttacc ccagaaatgc
     2521 cgccccggat ttctccaaaa acctaataag cttcatggaa cttggtggaa gtgactttct
     2581 acagagtgga gagaaccgtg gacacgtggc aatggcgctg accgtgtccc cgagccgaat
     2641 cgacgtgagg ggagaacgga gtatctgcgg tcatgtgacc ttccagagcg gcgtcgccag
     2701 tgtgcacgcg gtgaccccca gtttgggtct ctgtcacacg catactacct cggctctcca
     2761 catgctgaac tttatctttc gtggggatca taccgaaagt tgcaactacc aggtgtatat
     2821 aaagcctggt agactccccc cactttggac ctcatccaac caagacacac aaaaatggac
     2881 cgacttaaca acctcgccac ccagctcgag cagaaccccg ccaattcgta gataatggaa
     2941 tacaaatgga tatccagagt atacacatgg atagtataca ctgacacgac aattctgtat
     3001 ctctttatgt taactactgt gaggcattaa atagagcttg atatataaaa tgttacattt
     3061 cacagtctga acttttgcag attacctaat ttggtaagat attaattatg aactgaaagt
     3121 tgatggcatc cctaaatttg atgcaacgga atgcgtgcga tagtgaatca ttgctaacag
     3181 atcaagtaat cgtatcaatc gttccacgtc ttatccagac tcgatttccg ccctaaccta
     3241 aggaaactgc tgagtaattt cgaggttggt tcctaacaaa agccaagaaa cgcgccgatg
     3301 gtttagtggt aaaatccatc gttgccatcg atgggccccc ggttcgattc cgggtcggcg
     3361 caggttggtt agcagacgca gacggagact ggttttagag ctagaaatag caagttaaaa
     3421 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtggtgc tttttttgtg
     3481 tgggtacggt atcgcgtgca ttcgcggccg caaatttaaa taaaatgaag tgaagttcct
     3541 atactttcta gagaatagga acttctatag tgagtcgaat aagggcgaca caaaatttat
     3601 tctaaatgca taataaatac tgataacatc ttatagtttg tattatattt tgtattatcg
     3661 ttgacatgta taattttgat atcaaaaact gattttccct ttattatttt cgagatttat
     3721 tttcttaatt ctctttaaca aactagaaat attgtatata caaaaaatca taaataatag
     3781 atgaatagtt taattatagg tgttcatcaa tcgaaaaagc aacgtatctt atttaaagtg
     3841 cgttgctttt ttctcattta taaggttaaa taattctcat atatcaagca aagtgacagg
     3901 cgcccttaaa tattctgaca aatgctcttt ccctaaactc cccccataaa aaaacccgcc
     3961 gaagcgggtt tttacgttat ttgcggatta acgattactc gttatcagaa ccgcccaggg
     4021 ggcccgagct taagactggc cgtcgtttta caacacagaa agagtttgta gaaacgcaaa
     4081 aaggccatcc gtcaggggcc ttctgcttag tttgatgcct ggcagttccc tactctcgcc
     4141 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4201 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4501 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtgggc
     4741 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4861 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4921 gatcttttct acggggtctg acgctcagtg gaacgacgcg cgcgtaactc acgttaaggg
     4981 attttggtca tgagcttgcg ccgtcccgtc aagtcagcgt aatgctctgc ttttaccaat
     5041 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5101 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagcgctg
     5161 cgatgatacc gcgagaacca cgctcaccgg ctccggattt atcagcaata aaccagccag
     5221 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5281 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5341 ccatcgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5401 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5461 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5521 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5581 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5641 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5701 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5761 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5821 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5881 gttgaatact catattcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5941 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gtcagtgtta
     6001 caaccaatta accaattctg aacattatcg cgagcccatt tatacctgaa tatggctcat
     6061 aacacccctt gtttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac
     6121 tcagaagtga aacgccgtag cgccgatggt agtgtgggga ctccccatgc gagagtaggg
     6181 aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgcccggg
     6241 ctaattatgg ggtgtcgccc ttattcgact ctatagtgaa gttcctattc tctagaaagt
     6301 ataggaactt ctgaagtggg gatttaaat
//
