LOCUS       Exported                6840 bp ds-DNA     circular SYN 19-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV.CAG.SF-Venus-iGluSnFR.A184V
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 19, 2017 from SnapGene Viewer 3.0.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    260..427
                     /note="ITR"
     misc_feature    454..1388
                     /note="CBA promoter"
     misc_feature    1259..1281
                     /note=""
     misc_feature    1402..1464
                     /note="IgG K-leader"
     misc_feature    1465..1470
                     /note="MCS"
     misc_feature    1471..2217
                     /note="YbeJ 5-253"
     misc_feature    2008..2010
                     /note="A184V"
     misc_feature    2218..2493
                     /note="cpGFP 147-238"
     misc_feature    2236..2238
                     /note="M153T (SF)"
     misc_feature    2290..2292
                     /note="I171V (SF)"
     misc_feature    2302..2304
                     /note="G175S (SF)"
     misc_feature    2317..2319
                     /note="Y180D (SF)"
     misc_feature    2386..2388
                     /note="T203Y (Venus)"
     misc_feature    2395..2397
                     /note="A206V"
     misc_feature    2494..2511
                     /note="Linker"
     misc_feature    2512..2949
                     /note="cpSFGFP 1-146"
     misc_feature    2599..2601
                     /note="S30R"
     misc_feature    2626..2628
                     /note="Y38N Superfolder"
     misc_feature    2647..2649
                     /note="F46L (Venus)"
     misc_feature    2704..2706
                     /note="T65Y (GYG Chromophore)"
     misc_feature    2725..2727
                     /note="S72A (Venus)"
     misc_feature    2788..2790
                     /note="I93V"
     misc_feature    2824..2826
                     /note="N105T"
     misc_feature    2950..3027
                     /note="YbeJ 254-279"
     misc_feature    3040..3072
                     /note="Myc Epitope"
     misc_feature    3073..3219
                     /note="PDGFR 513-561"
     misc_feature    3235..3823
                     /note="WPRE"
     misc_feature    3845..4083
                     /note="PolyA"
     misc_feature    4111..4283
                     /note="ITR"
     misc_feature    5041..5898
                     /note="Amp-R"
ORIGIN
        1 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
       61 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      121 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      181 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt
      241 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      301 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      361 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      421 atctacgtag ccatgctcta ggaagatcgt accattgacg tcaataatga cgtatgttcc
      481 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggactatt tacggtaaac
      541 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      601 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      661 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
      721 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
      781 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc
      841 ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag
      901 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
      961 gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg ccccgctccg
     1021 ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg
     1081 ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt
     1141 cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga
     1201 gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg
     1261 ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc
     1321 ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc
     1381 aaagaattgg atcccgccac catggagaca gacacactcc tgctatgggt actgctgctc
     1441 tgggttccag gttccactgg tgacagatct gccgcaggca gcacgctgga caaaatcgcc
     1501 aaaaacggtg tgattgtcgt cggtcaccgt gaatcttcag tgcctttctc ttattacgac
     1561 aatcagcaaa aagtggtggg ttactcgcag gattactcca acgccattgt tgaagcagtg
     1621 aaaaagaaac tcaacaaacc ggacttgcag gtaaaactga ttccgattac ctcacaaaac
     1681 cgtattccac tgctgcaaaa cggcactttc gattttgaat gtggttctac caccaacaac
     1741 gtcgaacgcc aaaaacaggc ggctttctct gacactattt tcgtggtcgg tacgcgcctg
     1801 ttgaccaaaa agggtggcga tatcaaagat tttgccaacc tgaaagacaa agccgtagtc
     1861 gtcacttccg gcactacctc tgaagttttg ctcaacaaac tgaatgaaga gcaaaaaatg
     1921 aatatgcgca tcatcagcgc caaagatcac ggtgactctt tccgcaccct ggaaagcggt
     1981 cgtgccgttg cctttatgat ggatgacgtg ctgctggccg gtgaacgtgc gaaagcgaag
     2041 aaaccagaca actgggaaat cgtcggcaag ccgcagtctc aggaggccta cggttgtatg
     2101 ttgcgtaaag atgatccgca gttcaaaaag ctgatggatg acaccatcgc tcaggtgcag
     2161 acctccggtg aagcggaaaa atggtttgat aagtggttca aaaatccaat tctggtgagc
     2221 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggcgaa cttcaagatc
     2281 cgccacaacg tggaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     2341 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctatca gtccgtgctg
     2401 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     2461 gggatcactc tcggcatgga cgagctgtac aagggcggta ccggagggag catgagcaag
     2521 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     2581 ggccacaagt tcagcgtgcg cggcgagggc gagggcgatg ccaccaacgg caagctgacc
     2641 ctgaagctca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     2701 ctgggctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     2761 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcagctt caaggacgac
     2821 ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     2881 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     2941 aactttaaca accccctgaa catgaatttc gaactgtcag acgaaatgaa agcactgttc
     3001 aaagaaccga atgacaaggc actgaaactg caggtcgacg aacaaaaact catctcagaa
     3061 gaggatctga atgctgtggg ccaggacacg caggaggtca tcgtggtgcc acactccttg
     3121 ccctttaagg tggtggtgat ctcagccatc ctggccctgg tggtgctcac catcatctcc
     3181 cttatcatcc tcatcatgct ttggcagaag aagccacgtt agaagcttat cgataatcaa
     3241 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3301 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3361 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3421 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3481 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3541 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3601 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3661 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3721 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3781 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgaccc
     3841 gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     3901 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     3961 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     4021 gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     4081 atcgataagg atcttcctag agcatggcta cgtagataag tagcatggcg ggttaatcat
     4141 taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     4201 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     4261 gagcgagcga gcgcgcagcc ttaattaacc taattcactg gccgtcgttt tacaacgtcg
     4321 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     4381 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     4441 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4501 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4561 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4621 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4681 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4741 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4801 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4861 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     4921 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4981 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5041 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5101 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5161 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5221 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5281 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5341 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5401 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5461 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5521 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5581 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5641 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5701 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5761 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5821 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5881 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5941 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6001 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6061 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6181 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6421 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6481 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6721 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6781 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
//
