LOCUS       Exported                6252 bp ds-DNA     circular SYN 22-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAVU6+27-tCorn
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Jaffrey Lab
  TITLE     Direct Submission
  JOURNAL   Exported Dec 22, 2017 from SnapGene 3.0.0b
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1035..1365)
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      1049..1184
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        1398..1638
                     /label=U6 Promoter
                     /note="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    1647..1673
                     /note="27"
     misc_feature    1680..1712
                     /label=tRNA 5' stem(1)
                     /note="tRNA 5' stem"
     misc_feature    1687..1697
                     /note="A-box of tRNA promoter"
     misc_feature    1713..1742
                     /note="Corn"
     misc_feature    1743..1780
                     /label=tRNA 3' stem
                     /note="tRNA 3' stem"
     misc_feature    1756..1766
                     /note="B-box of tRNA promoter"
     misc_feature    1782..1806
                     /note="terminator"
     polyA_signal    1891..2012
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2321..2749)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2776..2880
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             2881..3741
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4943..5077
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(5502..5522)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     intron          5652..5717
                     /note="small t intron"
                     /note="simian virus 40 (SV40) small t antigen intron"
ORIGIN
        1 tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg
       61 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg
      121 gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct
      181 ggcgcgagcc cctgatgctc ttgtccagat catcctgatc gacaagaccg gcttccatcc
      241 gagtacgtgc tcgctcgatg cgatgttcgc ttggtggtcg aatgggcagg tagccggatc
      301 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
      361 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
      421 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
      481 ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag
      541 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
      601 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
      661 tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc
      721 cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc
      781 aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc
      841 ccagtctagc tatcggcatg taagcccact gcaagctacc tgctttctct ttgcgcttgc
      901 gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg
      961 cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg
     1021 gcacggtgaa gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
     1081 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     1141 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     1201 gggactatgg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     1261 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     1321 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagccggat
     1381 ccaaggtcgg gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat
     1441 acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag atattagtac
     1501 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     1561 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     1621 atatcttgtg gaaaggacga aacaccgtgc tcgcttcggc agcacatata ctagtcgacg
     1681 cccggatagc tcagtcggta gagcagcggc cgcgaggaag gaggtctgag gaggtcactg
     1741 cggccgcggg tccagggttc aagtccctgt tcgggcgcca tctagagcgg acttcggtcc
     1801 gctttttact aggacctgca ggcatgcaag cttgacgtcg gttaccgata tccatatggc
     1861 ggccgcatcg atctcgagcc gcggactagt aacttgttta ttgcagctta taatggttac
     1921 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     1981 tgtggtttgt ccaaactcat caatgtatct tatcatgtct tacgtagata agtagcatgg
     2041 cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg
     2101 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     2161 ggcggcctca gtgagcgagc gagcgcgcag agagggagtg gccaaagatc tctggcgtaa
     2221 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggctaatg
     2281 ggaaattgta aacgttaata ttttgttaat attttgttaa aattcgcgtt aaatttttgt
     2341 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     2401 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     2461 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     2521 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     2581 cctaaaggga tgccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     2641 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     2701 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     2761 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2821 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2881 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2941 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3001 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     3061 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     3121 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3181 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3241 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3301 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3361 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3421 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3481 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3541 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3601 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3661 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3721 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3781 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3841 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3901 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3961 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4021 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     4081 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4141 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4201 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcaacac agccagcttg
     4261 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     4321 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4381 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4441 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4501 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4561 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4621 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4681 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagagat
     4741 ctttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     4801 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agagagggag
     4861 tggccaactc catcactagg ggttcctgga ggggtggagt cgtgacgtga attacgtcat
     4921 agggttaggg aggtcctgga tcgatccaga catgataaga tacattgatg agtttggaca
     4981 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     5041 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     5101 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     5161 atgtggtatg gctgattatg atctctagtc aaggcactat acatcaaata ttccttatta
     5221 acccctttac aaattaaaaa gctaaaggta cacaattttt gagcatagtt attaatagca
     5281 gacactctat gcctgtgtgg agtaagaaaa aacagtatgt tatgattata actgttatgc
     5341 ctacttataa aggttacaga atatttttcc ataattttct tgtatagcag tgcagctttt
     5401 tcctttgtgg tgtaaatagc aaagcaagca agagttctat tactaaacac agcatgactc
     5461 aaaaaactta gcaattctga aggaaagtcc ttggggtctt ctacctttct cttctttttt
     5521 ggaggagtag aatgttgaga gtcagcagta gcctcatcat cactagatgg catttcttct
     5581 gagcaaaaca ggttttcctc attaaaggca ttccaccact gctcccattc atcagttcca
     5641 taggttggaa tctaaaatac acaaacaatt agaatcagta gtttaacaca ttatacactt
     5701 aaaaatttta tatttacctt agagctttaa atctctgtag gtagtttgtc caattatgtc
     5761 acaccacaga agtaaggttc cttcacaaag atccgggacc aaagcggcca tcgtgcctcc
     5821 ccactcctgc agttcggggg catggatgcg cggatagccg ctgctggttt cctggatgcc
     5881 gacggatttg cactgccggt agaactccgc gaggtcgtcc agcctcaggc agcagctgaa
     5941 ccaactcgcg aggggatcga gcccggggtg ggcgaagaac tccagcatga gatccccgcg
     6001 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     6061 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
     6121 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     6181 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     6241 tcttcagcaa ta
//
