LOCUS Exported 3344 bp ds-DNA circular SYN 01-MAR-2018 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3344) AUTHORS MIT TITLE Direct Submission JOURNAL Exported Mar 1, 2018 from SnapGene 4.1.6 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3344 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 1..589 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 617..635 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" RBS 667..689 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" misc_feature 697..2277 /label=banana terminator 2343..2390 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(2408..3223) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" ORIGIN 1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 601 cgcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga 661 aataattttg tttaacttta agaaggagat atacatatga atgaaattga tgaaaagaac 721 caagccccag tacagcaaga atgcttaaaa gaaatgattc agaacggaca tgcccgtcgt 781 atggggtcgg tcgaggacct gtatgtggcg cttaaccgtc agaaccttta tcgtaacttt 841 tgtacatacg gggagctgag tgactactgc acacgtgacc aacttacact ggcgctgcgt 901 gagatttgcc tgaaaaatcc tactctttta catatcgtgt tgcccacgcg ctggccgaac 961 cacgaaaact actatcgcag ttccgagtat tactcgcgtc cacatcccgt ccacgactac 1021 atctccgtat tacaagaatt gaaacttagt ggagtagtgc ttaatgaaca acctgagtac 1081 tcagcagtga tgaagcagat cttagaagaa ttcaagaatt caaagggcag ttatacagcg 1141 aaaattttta aacttacaac cacactgaca attccttact tcggcccgac tggacccagt 1201 tggcgcctta tctgtcttcc ggaggaacat actgagaaat ggaagaaatt tattttcgtc 1261 agcaaccatt gcatgtcgga cggacgcagt tcaattcatt ttttccatga tttacgcgac 1321 gagttaaata atattaagac ccctcctaaa aagttagact atatttttaa gtacgaggag 1381 gattaccagt tgcttcgtaa acttcccgaa ccaatcgaaa aggtgatcga tttccgccct 1441 ccgtacttat ttatccccaa aagcctttta tcgggattca tttacaacca tcttcgtttc 1501 agcagcaaag gggtctgtat gcgcatggac gacgtggaaa aaactgacga tgttgtaaca 1561 gaaattatta acatttcccc gactgaattc caagccatta aggcgaacat taaatcaaat 1621 atccaaggca aatgtacgat tacccccttt ctgcacgtgt gttggttcgt gagccttcac 1681 aagtggggca aattttttaa gcccttaaat tttgagtggc ttacggatat tttcatcccc 1741 gcagattgtc gttcccaatt acctgatgat gatgagatgc gtcagatgta ccgttacggt 1801 gccaacgtag gtttcattga ctttacgcca tggatttcag aatccgacat gaacgataac 1861 aaagaaaatt tctggcctct gattgaacat taccatgaag ttatttcaga agcactgcgt 1921 aataagaaac accttcacgg attagggttc aacatccaag gatttgtaca aaagtacgtt 1981 aacatcgaca aggtgatgtg cgatcgtgca attggcaaac gtcgtggcgg tacactgttg 2041 tccaatgtgg gactgtttaa tcagctggag gagcctgacg ctaaatacag catttgtgac 2101 cttgccttcg gtcagtttca aggcagctgg caccaagctt tttcattagg ggtgtgtagc 2161 actaatgtaa aagggatgaa cattgtggtc gcgagcacga aaaatgttgt ggggtcacag 2221 gaatcattag aagaactgtg cagtatttac aaagctttgt tgctgggtcc ataataagtc 2281 gaccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat 2341 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaagcca 2401 attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat 2461 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc 2521 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa 2581 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag 2641 tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa 2701 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc 2761 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag 2821 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat 2881 caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc 2941 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca 3001 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt 3061 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt 3121 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta 3181 atcgcggctt cgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac 3241 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt 3301 aacatcagag attttgagac acaacgtgag atcaaaggat cttc //