LOCUS Exported 4143 bp ds-DNA circular SYN 03-JAN-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4143) AUTHORS MIT TITLE Direct Submission JOURNAL Exported Jan 3, 2018 from SnapGene 4.0.7 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4143 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(14..45) /label=ecarin_to_pNP1_V_R promoter 26..44 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 135..1985 /label=ecarin primer_bind 1966..2013 /label=ecarin_to_pNP1_V_F terminator 2135..2182 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind complement(2193..2217) /label=pNP1_Lin_R (71C) rep_origin complement(2400..2988) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3160..4020) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4021..4125) /gene="bla" /label=AmpR promoter ORIGIN 1 gctagtggtg ctagccccgc gaaattaata cgactcacta tagggagacc caagctggct 61 agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc 121 tgcagatatc caccatgata cagattcttc tggtaataat atgtttggcc gtattccctt 181 atcaaggttg cagtattatt cttggtagtg gcaatgtcaa tgattacgaa gtggtgtacc 241 cccaaaaggt tactgctctt ccaaaagggg ctgtgcagca gcccgagcag aaatacgagg 301 acgcaatgca gtatgagttt gaggtgaaag gagagccagt agtgttacac ttggaaaaga 361 ataaagaact gttctcggag gattactcag agacgcacta ctcttcggat gatagagaga 421 tcaccacaaa tcctagcgta gaggaccatt gctattatca tggtcgcata caaaacgatg 481 ctgagtcgac ggcctccatt tcggcgtgta atggactgaa aggtcatttt aaattgcggg 541 gtgaaactta tttcatagag ccacttaaga ttcctgacag tgaggcgcat gccgtctaca 601 agtacgagaa tatcgaaaat gaagacgagg cacctaaaat gtgtggagtt actcaggata 661 attgggaaag tgatgagccg atcaaaaaaa cgcttggatt aatagttcct cctcacgaaa 721 gaaagttcga gaagaagttt attgaattag tcgtcgtagt ggatcattca atggttacga 781 aatacaacaa cgactcaacg gcgatccgta cctggatata tgaaatgctg aacacggtca 841 atgagattta tttgcctttc aatataagag tagcattagt agggttagag ttttggtgta 901 acggagactt aattaacgtc acttcgacgg cagacgatac attgcactca ttcggggagt 961 ggcgggcgag tgatctgtta aaccgcaaaa gacacgatca tgctcagttg ttgacgaacg 1021 tcaccttaga tcactctacg ctgggtatta cgtttgtgta tggaatgtgt aaatcggatc 1081 ggtccgtgga gctgatttta gactattcaa acatcacgtt caacatggcc tacataattg 1141 cacatgagat gggtcacagt ctgggaatgt tacatgacac gaaattctgc acttgtggag 1201 ctaagccatg catcatgttt ggcaaagaaa gtatcccacc ccccaaagaa tttagttcat 1261 gttcttatga tcagtataac aagtatttgt tgaagtataa tcctaaatgc atacttgacc 1321 ctccgttgcg gaaagacata gcatcgccgg ctgtgtgtgg gaatgagatc tgggaggaag 1381 gggaagaatg tgattgcggt tcaccagcgg actgtcgcaa cccctgttgt gacgctgcaa 1441 catgcaagct gaaaccagga gcggagtgcg ggaatggtga gtgttgtgat aagtgcaaga 1501 tacgcaaagc cggaactgag tgtagaccgg caagagacga ctgcgatgtg gctgaacact 1561 gtactggaca atccgcggaa tgcccaagaa atgagtttca gcgcaacggt caaccatgtc 1621 ttaataactc gggatactgc tacaatgggg actgtcctat tatgctgaac cagtgcattg 1681 cgctgttctc gccctcagct acagttgcgc aggactcttg cttccaaaga aacttacaag 1741 ggtcatacta cggctattgt accaaagaaa ttggatacta cggaaaaaga ttcccgtgtg 1801 ctccgcagga tgtaaagtgc ggacgtctgt attgtctgga caattctttt aagaagaaca 1861 tgagatgtaa gaacgattac tcctatgcgg acgagaacaa aggtatagtt gagccgggca 1921 ccaagtgtga agacgggaag gtttgcatca atcgtaaatg tgtagatgta aacactgcct 1981 actgaaggat cccgggaatt ctcgagtaag gttaacctgc aggaggcctt taattaaggt 2041 ggtgcggccg cgctagcggt cccgggggat cgatccggct gctaacaaag cccgaaagga 2101 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa 2161 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggaagct tggcactggc 2221 cgaccggggt cgagcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 2281 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 2341 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 2401 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 2461 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 2521 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 2581 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 2641 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 2701 tatcgtcttg agtccaaccc gctaagacac gacttatcgc cactggcagc agccactggt 2761 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 2821 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 2881 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 2941 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 3001 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacagatccg ggattttggt 3061 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 3121 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 3181 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 3241 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 3301 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 3361 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 3421 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 3481 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 3541 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 3601 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 3661 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 3721 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 3781 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 3841 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 3901 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 3961 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 4021 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 4081 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 4141 agt //