LOCUS Exported 2834 bp ds-DNA circular SYN 01-MAR-2018 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2834) AUTHORS MIT TITLE Direct Submission JOURNAL Exported Mar 1, 2018 from SnapGene 4.1.6 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2834 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 1..589 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 617..635 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" RBS 667..689 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" misc_feature 697..1767 /label=Trx-Bx terminator 1833..1880 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(1898..2713) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" ORIGIN 1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 601 cgcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga 661 aataattttg tttaacttta agaaggagat atacatatga gtgataagat tatccatctt 721 actgacgact ccttcgatac cgacgtcctg aaagcggatg gagctatcct tgtggacttt 781 tgggctgagt ggtgtggccc atgtaaaatg atcgccccga ttctggatga gattgcggac 841 gaatatcaag gaaagttgac cgttgctaag cttaacattg accaaaatcc aggaaccgct 901 ccaaaatacg gtattcgtgg catcccaacg cttttgcttt tcaaaaatgg tgaagttgcg 961 gctaccaaag taggtgccct gtcaaaaggt caattaaaag aatttctgga tgcgaacttg 1021 gcaggttctg gttctggcca catgcaccac catcatcatc atagcagtgg tgtaattggg 1081 ggggacgagt gcgacatcaa cgagcacccg ttcttagcat tcatgtatta ttctccgcgc 1141 tacttctgtg gtatgacgct gattaaccaa gagtgggtat tgactgctgc acactgtaac 1201 cgtcgtttca tgcgcattca cctgggaaag catgcaggga gcgtggctaa ttatgatgaa 1261 gttgttcgtt atcctaaaga aaaatttatt tgtccaaaca agaaaaaaaa cgtgattacc 1321 gacaaggata ttatgctgat ccgtttggac cgccctgtca aaaattcgga acacattgcc 1381 ccattgagcc tgccctcaaa ccccccctcc gtagggtcgg tgtgtcgtat tatggggtgg 1441 ggtgcaatca cgacgtcgga agatacgtat cccgatgtac cacattgcgc taacatcaat 1501 cttttcaaca atacagtttg ccgcgaagcg tataacgggc tgcccgcgaa gactctttgt 1561 gcgggcgtct tgcagggtgg cattgacaca tgtggcggtg actcgggagg tccgttaatt 1621 tgtaatgggc agtttcaagg aatcttgagc tggggctcgg acccatgtgc ggaacctcgc 1681 aaacccgcat tttatacaaa ggtattcgat taccttccgt ggatccagtc gattatcgcc 1741 gggaacaaga ctgcaacttg tccttaagtc gaccggctgc taacaaagcc cgaaaggaag 1801 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac 1861 gggtcttgag gggttttttg ctgaaagcca attctgatta gaaaaactca tcgagcatca 1921 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt 1981 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc 2041 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa 2101 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa 2161 gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat 2221 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc 2281 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg 2341 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg 2401 ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct 2461 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa 2521 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc 2581 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc 2641 catataaatc agcatccatg ttggaattta atcgcggctt cgagcaagac gtttcccgtt 2701 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc 2761 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtgag 2821 atcaaaggat cttc //