LOCUS       Exported                2834 bp ds-DNA     circular SYN 01-MAR-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Mar 1, 2018 from SnapGene 4.1.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        617..635
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             667..689
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    697..1767
                     /label=Trx-Bx
     terminator      1833..1880
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1898..2713)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga
      661 aataattttg tttaacttta agaaggagat atacatatga gtgataagat tatccatctt
      721 actgacgact ccttcgatac cgacgtcctg aaagcggatg gagctatcct tgtggacttt
      781 tgggctgagt ggtgtggccc atgtaaaatg atcgccccga ttctggatga gattgcggac
      841 gaatatcaag gaaagttgac cgttgctaag cttaacattg accaaaatcc aggaaccgct
      901 ccaaaatacg gtattcgtgg catcccaacg cttttgcttt tcaaaaatgg tgaagttgcg
      961 gctaccaaag taggtgccct gtcaaaaggt caattaaaag aatttctgga tgcgaacttg
     1021 gcaggttctg gttctggcca catgcaccac catcatcatc atagcagtgg tgtaattggg
     1081 ggggacgagt gcgacatcaa cgagcacccg ttcttagcat tcatgtatta ttctccgcgc
     1141 tacttctgtg gtatgacgct gattaaccaa gagtgggtat tgactgctgc acactgtaac
     1201 cgtcgtttca tgcgcattca cctgggaaag catgcaggga gcgtggctaa ttatgatgaa
     1261 gttgttcgtt atcctaaaga aaaatttatt tgtccaaaca agaaaaaaaa cgtgattacc
     1321 gacaaggata ttatgctgat ccgtttggac cgccctgtca aaaattcgga acacattgcc
     1381 ccattgagcc tgccctcaaa ccccccctcc gtagggtcgg tgtgtcgtat tatggggtgg
     1441 ggtgcaatca cgacgtcgga agatacgtat cccgatgtac cacattgcgc taacatcaat
     1501 cttttcaaca atacagtttg ccgcgaagcg tataacgggc tgcccgcgaa gactctttgt
     1561 gcgggcgtct tgcagggtgg cattgacaca tgtggcggtg actcgggagg tccgttaatt
     1621 tgtaatgggc agtttcaagg aatcttgagc tggggctcgg acccatgtgc ggaacctcgc
     1681 aaacccgcat tttatacaaa ggtattcgat taccttccgt ggatccagtc gattatcgcc
     1741 gggaacaaga ctgcaacttg tccttaagtc gaccggctgc taacaaagcc cgaaaggaag
     1801 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
     1861 gggtcttgag gggttttttg ctgaaagcca attctgatta gaaaaactca tcgagcatca
     1921 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     1981 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     2041 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     2101 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     2161 gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     2221 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
     2281 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     2341 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     2401 ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     2461 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     2521 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     2581 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     2641 catataaatc agcatccatg ttggaattta atcgcggctt cgagcaagac gtttcccgtt
     2701 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
     2761 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtgag
     2821 atcaaaggat cttc
//
