LOCUS       Exported                4645 bp ds-DNA     circular SYN 10-JAN-2005
DEFINITION  synthetic circular DNA
ACCESSION   Not-Specified.
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 4, 2018 from SnapGene 4.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..602
                     /gene="CMV promoter"
     misc_feature    679..1065
                     /gene="NCre-ORF"
     misc_feature    682..705
                     /gene="FLAG tag"
     misc_feature    721..861
                     /gene="GCN4-ORF"
     misc_feature    862..897
                     /gene="Linker"
     misc_feature    919..939
                     /gene="NLS"
     misc_feature    940..1065
                     /gene="iCre_aa19-59"
     misc_feature    1178..1561
                     /gene="SV40 poly A"
     misc_feature    complement(1699..2005)
                     /gene="f1 origin"
     misc_feature    2029..2154
                     /gene="bla promoter"
     misc_feature    2174..2512
                     /gene="SV40 promoter"
     misc_feature    2547..3338
                     /gene="neo/kan resistance ORF"
     misc_feature    3339..3797
                     /gene="HSV-TK poly A"
     misc_feature    3926..4593
                     /gene="pUC origin"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgccaccat ggattacaag gatgacgacg ataagagccc gggcggatcc
      721 aacactgaag ccgccaggcg ttctcgtgcg agaaagttgc aaagaatgaa acaacttgaa
      781 gacaaggttg aagaattgct ttcgaaaaat tatcacttgg aaaatgaggt tgccagatta
      841 aagaaattag ttggcgaacg cgccagccct agcaaccctg gcgccagcaa cggcagcctg
      901 caggaattca gcggccctcc caagaagaag aggaaggtga cctctgatga agtcaggaag
      961 aacctgatgg acatgttcag ggacaggcag gccttctctg aacacacctg gaagatgctc
     1021 ctgtctgtgt gcagatcctg ggctgcctgg tgcaagctga actagaagct tatcgatacc
     1081 gtcgacctcg agggggggcc cggtacctta attaattaag gtaccaggta agtgtaccca
     1141 attcgcccta tagtgagtcg tattacaatt cactcgatcg cccttcccaa cagttgcgca
     1201 gcctgaatgg cgaatggaga tccaattttt aagtgtataa tgtgttaaac tactgattct
     1261 aattgtttgt gtattttaga ttcacagtcc caaggctcat ttcaggcccc tcagtcctca
     1321 cagtctgttc atgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     1381 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     1441 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     1501 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     1561 acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     1621 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     1681 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     1741 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     1801 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     1861 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     1921 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     1981 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     2041 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2101 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag aatcctgagg
     2161 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     2221 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     2281 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     2341 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     2401 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     2461 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     2521 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     2581 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     2641 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     2701 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     2761 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     2821 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     2881 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     2941 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     3001 ttgtcgatca ggatgatctg gacgaagaac atcaggggct cgcgccagcc gaactgttcg
     3061 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     3121 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     3181 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagaac
     3241 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     3301 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     3361 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     3421 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     3481 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
     3541 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     3601 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     3661 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     3721 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     3781 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     3841 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     3901 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3961 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4021 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4081 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4141 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4201 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4261 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4321 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4381 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4441 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4501 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4561 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     4621 ttctgtggat aaccgtatta ccgcc
//
