LOCUS       Exported                5599 bp ds-DNA     circular SYN 10-JAN-2005
DEFINITION  synthetic circular DNA
ACCESSION   Not-Specified.
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 4, 2018 from SnapGene 4.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..602
                     /gene="CMV promoter"
     misc_feature    679..2014
                     /gene="NCre-ERT2-ORF"
     misc_feature    682..705
                     /gene="FLAG tag"
     misc_feature    721..861
                     /gene="GCN4-ORF"
     misc_feature    919..939
                     /gene="NLS"
     misc_feature    940..1062
                     /label=iCre_aa19-59
                     /note="iCre_aa19-59"
     misc_feature    1069..2010
                     /label=ERT2
                     /note="ERT2"
     misc_feature    2132..2515
                     /gene="SV40 poly A"
     misc_feature    complement(2653..2959)
                     /gene="f1 origin"
     misc_feature    2983..3108
                     /gene="bla promoter"
     misc_feature    3128..3466
                     /gene="SV40 promoter"
     misc_feature    3501..4292
                     /gene="neo/kan resistance ORF"
     misc_feature    4293..4751
                     /gene="HSV-TK poly A"
     misc_feature    4880..5547
                     /gene="pUC origin"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgccaccat ggattacaag gatgacgacg ataagagccc gggcggatcc
      721 aacactgaag ccgccaggcg ttctcgtgcg agaaagttgc aaagaatgaa acaacttgaa
      781 gacaaggttg aagaattgct ttcgaaaaat tatcacttgg aaaatgaggt tgccagatta
      841 aagaaattag ttggcgaacg cgccagccct agcaaccctg gcgccagcaa cggcagcctg
      901 caggaattca gcggccctcc caagaagaag aggaaggtga cctctgatga agtcaggaag
      961 aacctgatgg acatgttcag ggacaggcag gccttctctg aacacacctg gaagatgctc
     1021 ctgtctgtgt gcagatcctg ggctgcctgg tgcaagctga acactagttc tgctggagac
     1081 atgagagctg ccaacctttg gccaagcccg ctcatgatca aacgctctaa gaagaacagc
     1141 ctggccttgt ccctgacggc cgaccagatg gtcagtgcct tgttggatgc tgagcccccc
     1201 atactctatt ccgagtatga tcctaccaga cccttcagtg aagcttcgat gatgggctta
     1261 ctgaccaacc tggcagacag ggagctggtt cacatgatca actgggcgaa gagggtgcca
     1321 ggctttgtgg atttgaccct ccatgatcag gtccaccttc tagaatgtgc ctggctagag
     1381 atcctgatga ttggtctcgt ctggcgctcc atggagcacc cagtgaagct actgtttgct
     1441 cctaacttgc tcttggacag gaaccaggga aaatgtgtag agggcatggt ggagatcttc
     1501 gacatgctgc tggctacatc atctcggttc cgcatgatga atctgcaggg agaggagttt
     1561 gtgtgcctca aatctattat tttgcttaat tctggagtgt acacatttct gtccagcacc
     1621 ctgaagtctc tggaagagaa ggaccatatc caccgagtcc tggacaagat cacagacact
     1681 ttgatccacc tgatggccaa ggcaggcctg accctgcagc agcagcacca gcggctggcc
     1741 cagctcctcc tcatcctctc ccacatcagg cacatgagta acaaaggcat ggagcatctg
     1801 tacagcatga agtgcaagaa cgtggtgccc ctctatgacc tgctgctgga ggcggcggac
     1861 gcccaccgcc tacatgcgcc cactagccgt ggaggggcat ccgtggagga gacggaccaa
     1921 agccacttgg ccactgcggg ctctacttca tcgcattcct tgcaaaagta ttacatcacg
     1981 ggggaggcag agggtttccc tgccacagtc tgaactagta agcttatcga taccgtcgac
     2041 ctcgaggggg ggcccggtac cttaattaat taaggtacca ggtaagtgta cccaattcgc
     2101 cctatagtga gtcgtattac aattcactcg atcgcccttc ccaacagttg cgcagcctga
     2161 atggcgaatg gagatccaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
     2221 ttgtgtattt tagattcaca gtcccaaggc tcatttcagg cccctcagtc ctcacagtct
     2281 gttcatgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     2341 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     2401 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     2461 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt
     2521 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     2581 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     2641 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     2701 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     2761 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     2821 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     2881 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     2941 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     3001 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3061 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagaatcct gaggcggaaa
     3121 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     3181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     3241 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     3301 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3361 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3421 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     3481 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     3541 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     3601 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     3661 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     3721 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     3781 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     3841 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     3901 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     3961 atcaggatga tctggacgaa gaacatcagg ggctcgcgcc agccgaactg ttcgccaggc
     4021 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     4081 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     4141 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gaacttggcg
     4201 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     4261 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     4321 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     4381 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     4441 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     4501 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     4561 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     4621 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     4681 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     4741 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4801 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4861 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4921 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4981 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5041 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5101 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5161 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5221 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5281 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5341 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5401 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5461 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5521 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5581 ggataaccgt attaccgcc
//
