LOCUS       Exported                6361 bp ds-DNA     circular SYN 10-JAN-2005
DEFINITION  synthetic circular DNA
ACCESSION   Not-Specified.
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 4, 2018 from SnapGene 4.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..602
                     /gene="CMV promoter"
     misc_feature    676..2781
                     /gene="ERT2-CCre-ORF"
     misc_feature    679..708
                     /gene="c-myc tag"
     misc_feature    724..1665
                     /label=ERT2
                     /note="ERT2"
     misc_feature    1708..1848
                     /gene="GCN4-ORF"
     misc_feature    1906..1926
                     /gene="NLS"
     misc_feature    1927..2781
                     /gene="iCre_aa60-343"
     misc_feature    2894..3277
                     /gene="SV40 poly A"
     misc_feature    complement(3415..3721)
                     /gene="f1 origin"
     misc_feature    3745..3870
                     /gene="bla promoter"
     misc_feature    3890..4228
                     /gene="SV40 promoter"
     misc_feature    4263..5054
                     /gene="neo/kan resistance ORF"
     misc_feature    5055..5513
                     /gene="HSV-TK poly A"
     misc_feature    5642..6309
                     /gene="pUC origin"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgccatgga gcagaaactc atctctgaag aggatctgag cccgggcgga
      721 tcctctgctg gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc
      781 tctaagaaga acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg
      841 gatgctgagc cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct
      901 tcgatgatgg gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg
      961 gcgaagaggg tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa
     1021 tgtgcctggc tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccagtg
     1081 aagctactgt ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc
     1141 atggtggaga tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg
     1201 cagggagagg agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca
     1261 tttctgtcca gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac
     1321 aagatcacag acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag
     1381 caccagcggc tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa
     1441 ggcatggagc atctgtacag catgaagtgc aagaacgtgg tgcccctcta tgacctgctg
     1501 ctggaggcgg cggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg
     1561 gaggagacgg accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa
     1621 aagtattaca tcacggggga ggcagagggt ttccctgcca cagtcgccag ccctagcaac
     1681 cctggcgcca gcaacggcag cggatccaac actgaagccg ccaggcgttc tcgtgcgaga
     1741 aagttgcaaa gaatgaaaca acttgaagac aaggttgaag aattgctttc gaaaaattat
     1801 cacttggaaa atgaggttgc cagattaaag aaattagttg gcgaacgcgc cagccctagc
     1861 aaccctggcg ccagcaacgg cagcctgcag gaattcagcg gccctcccaa gaagaagagg
     1921 aaggtgaaca ggaaatggtt ccctgctgaa cctgaggatg tgagggacta cctcctgtac
     1981 ctgcaagcca gaggcctggc tgtgaagacc atccaacagc acctgggcca gctcaacatg
     2041 ctgcacagga gatctggcct gcctcgccct tctgactcca atgctgtgtc cctggtgatg
     2101 aggagaatca gaaaggagaa tgtggatgct ggggagagag ccaagcaggc cctggccttt
     2161 gaacgcactg actttgacca agtcagatcc ctgatggaga actctgacag atgccaggac
     2221 atcaggaacc tggccttcct gggcattgcc tacaacaccc tgctgcgcat tgccgaaatt
     2281 gccagaatca gagtgaagga catctcccgc accgatggtg ggagaatgct gatccacatt
     2341 ggcaggacca agaccctggt gtccacagct ggtgtggaga aggccctgtc cctgggggtt
     2401 accaagctgg tggagagatg gatctctgtg tctggtgtgg ctgatgaccc caacaactac
     2461 ctgttctgcc gggtcagaaa gaatggtgtg gctgcccctt ctgccacctc ccaactgtcc
     2521 acccgggccc tggaagggat ctttgaggcc acccaccgcc tgatctatgg tgccaaggat
     2581 gactctgggc agagatacct ggcctggtct ggccactctg ccagagtggg tgctgccagg
     2641 gacatggcca gggctggtgt gtccatccct gaaatcatgc aggctggtgg ctggaccaat
     2701 gtgaacattg tgatgaacta catcagaaac ctggactctg agactggggc catggtgagg
     2761 ctgctcgagg atggggacta gaagcttatc gataccgtcg acctcgaggg ggggcccggt
     2821 accttaatta attaaggtac caggtaagtg tacccaattc gccctatagt gagtcgtatt
     2881 acaattcact cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggagatcca
     2941 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattca
     3001 cagtcccaag gctcatttca ggcccctcag tcctcacagt ctgttcatga tcataatcag
     3061 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     3121 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     3181 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3241 tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata
     3301 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     3361 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     3421 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3481 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     3541 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3601 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3661 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3721 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3781 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3841 tgcttcaata atattgaaaa aggaagaatc ctgaggcgga aagaaccagc tgtggaatgt
     3901 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3961 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4021 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4081 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4141 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4201 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     4261 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4321 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4381 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4441 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4501 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4561 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4621 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4681 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4741 aagaacatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4801 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4861 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4921 acatagcgtt ggctacccgt gatattgctg aagaacttgg cggcgaatgg gctgaccgct
     4981 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     5041 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     5101 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     5161 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     5221 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     5281 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     5341 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     5401 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     5461 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     5521 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5581 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5641 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5701 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5761 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     5821 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5881 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5941 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6001 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6061 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6121 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6181 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6241 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6301 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6361 c
//
