LOCUS       Exported               12672 bp ds-DNA     circular SYN 20-OCT-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pJMR52-pJMR50-Gent
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 12672)
  AUTHORS   Brian Pfleger Group
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 5, 2018 from SnapGene 3.1.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12672
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     gene            complement(62..1210)
                     /gene="trfA"
                     /note="trfA"
     CDS             complement(62..1210)
                     /codon_start=1
                     /transl_table=11
                     /gene="trfA"
                     /product="replication protein"
                     /note="trfA"
                     /db_xref="GI:525567860"
                     /protein_id="AGR55406.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      1603..2134
                     /note="oriV"
                     /note="incP origin of replication"
     terminator      2246..2254
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2267..2276)
                     /codon_start=1
                     /note="beta-lactamase"
                     /note="bla; ampicillin resistance"
                     /translation="HW"
     CDS             complement(2290..3467)
                     /note="GmR"
     CDS             complement(2550..3083)
                     /codon_start=1
                     /note="gentamicin-3-acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     misc_feature    3140..3158
                     /note="CMJ134"
     promoter        complement(3272..3300)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /note="Pc promoter"
                     /note="class 1 integron promoter"
     misc_feature    3272..3280
                     /note="-10 box"
     misc_feature    3295..3300
                     /note="-35 box"
     misc_feature    3512..3547
                     /note="FLAG tag /w G-S-G linker"
     CDS             complement(3548..7651)
                     /codon_start=1
                     /note="wtCas9"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    5132..5134
                     /note="HNH like active site"
     misc_feature    7622..7624
                     /note="RuvC-like active site"
     misc_feature    7660..7665
                     /note="RBS"
     misc_feature    7685
                     /note="TSP"
     misc_feature    7686..7745
                     /note="J23105 promoter, -60 to -1"
     misc_feature    7687..7721
                     /note="J23105 Variable region"
     terminator      7774..8039
                     /note="rrnB_terminator"
     terminator      7793..7836
                     /note="rrnB_T1_terminator"
     misc_feature    7992..8009
                     /note="pTrcHis_rev_primer"
     misc_feature    7992..8009
                     /note="pBAD_rev_primer"
     misc_feature    8040..8140
                     /note="Spacer Included in Qi et. al. 2013"
                     /note="Spacer Included in Qi et. al. 2013 ""Repurposing 
                     CRISPR..."""
     misc_feature    8141..8181
                     /note="S. pyogenes terminator"
     misc_feature    8182..8223
                     /note="cas9 Handle"
     misc_feature    8224..8243
                     /note="gRNA targeting region for pBR322 ori"
     misc_feature    8243
                     /note="PLtetO1 TSP"
     misc_feature    8250..8255
                     /note="-10"
     misc_feature    8254..8272
                     /note="tet operator"
     misc_feature    8264..8303
                     /note="rMP162 tail"
     misc_feature    8273..8278
                     /note="-35"
     misc_feature    8279..8297
                     /note="tet operator"
     misc_feature    8354..8431
                     /note="lacIq region"
     misc_feature    8402..8475
                     /note="rMP168"
     misc_feature    8404
                     /note="lacI TSP"
     gene            8432..9055
                     /note="tetR"
     misc_feature    9055..9114
                     /note="rMP167"
     misc_feature    9056..9082
                     /note="rMP160 overhang"
     terminator      9066..9099
                     /note="arcA terminator (bidirectional)"
     misc_feature    9083..9109
                     /note="rMP166 overhang"
     terminator      9189..9220
                     /note="TonB terminator"
                     /note="TonB terminator (in correct orientation if 
                     transcription is from left to right - this direction is 
                     somewhat more efficient than the reverse orientation, 
                     though the terminator is bidirectional)"
     CDS             complement(9271..10149)
                     /codon_start=1
                     /note="araC protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     misc_feature    10300..10328
                     /note="Pc"
     misc_feature    10425..10452
                     /note="ParaB"
     CDS             10504..10920
                     /codon_start=1
                     /note="gam protein"
                     /note="recombination;138"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             10926..11711
                     /codon_start=1
                     /note="bet protein"
                     /note="recombination;261"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             11708..12388
                     /codon_start=1
                     /note="exonuclease"
                     /note="exo"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     misc_feature    12378..12647
                     /note="tL3"
     CDS             12385..12567
                     /codon_start=1
                     /note="orf60a protein"
                     /note="60a"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEEI
                     NNKRGAVCTKHLLLS"
ORIGIN
        1 ttatacctag ggcgatgcag gtggctgctg aacccccagc cggaactgac cccacaaggc
       61 cctagcgttt gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc
      121 ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat
      181 ccgatccgca catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc
      241 tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga
      301 atttcgtgta gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc
      361 aacgggacgt tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt
      421 ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc
      481 attcctcggc cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct
      541 cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct
      601 gctgccacac cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca
      661 cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt
      721 tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg
      781 gccacggcgc aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt
      841 tcagcaacgc ggcctgcttg gcttcgctga cctgttttgc caggtcctcg ccggcggttt
      901 ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg
      961 ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag
     1021 ggggagccag ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg actatcgagc
     1081 cgacggactg gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg
     1141 catcctcggc ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg
     1201 tccgattcat tcaccctcct tgcgggattg ccccggaatt aattccccgg atcgatccgt
     1261 cgatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
     1321 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt
     1381 ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct
     1441 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca
     1501 gcaccgtttc tgcggactgg ctttctacgt ggctgccatt tttggggtga ggccgttcgc
     1561 ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc gggagggttc
     1621 gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt
     1681 taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc
     1741 cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa
     1801 atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg
     1861 cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca
     1921 ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg
     1981 aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg
     2041 cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca
     2101 agttttccgc gaggtatcca caacgccggc ggccctacat ggctctgctg tagtgagtgg
     2161 gttgcgctcc ggcagcggtc ctgatccccc gcagaaaaaa aggatctcaa gaagatcctt
     2221 tgatcttttc tacactagtg cttggattct caccttggtc tgacagttac caatgcgagg
     2281 gggggatcca ccttcgggag cgcctgaagc ccgttctgga cgccctgggg ccgttgaatc
     2341 gggatatgca ggccaaggcc gccgcgatca tcaaggccgt gggcgaaaag ctgctgacgg
     2401 aacagcggga agtccagcgc cagaaacagg cccagcgcca gcaggaacgc gggcgcgcac
     2461 atttccccga aaagtgccac ctggcggcgt tgtgacaatt taccgaacaa ctccgcggcc
     2521 gggaagccga tctcggcttg aacgaattgt taggtggcgg tacttgggtc gatatcaaag
     2581 tgcatcactt cttcccgtat gcccaacttt gtatagagag ccactgcggg atcgtcaccg
     2641 taatctgctt gcacgtagat cacataagca ccaagcgcgt tggcctcatg cttgaggaga
     2701 ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc cggagactgc gagatcatag
     2761 atatagatct cactacgcgg ctgctcaaac ctgggcagaa cgtaagccgc gagagcgcca
     2821 acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg gagcaagttc
     2881 ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga
     2941 ccgaaaagat caagagcagc ccgcatggat ttgacttggt cagggccgag cctacatgtg
     3001 cgaatgatgc ccatacttga gccacctaac tttgttttag ggcgactgcc ctgctgcgta
     3061 acatcgttgc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
     3121 gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca
     3181 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
     3241 gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa
     3301 ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcca tgggcaaata ttatacgcaa
     3361 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     3421 catgtcggca gaatgcttaa tgaattacaa cagtttttat gcatgcgcag tccagttacg
     3481 ctggagtctg aggctcgtcc tgaatgatat ctcatttatc atcatcatct ttataatcac
     3541 cgctgccgtc acctcctagc tgactcaaat caatgcgtgt ttcataaaga ccagtgatgg
     3601 attgatggat aagagtggca tctaaaactt cttttgtaga cgtatatcgt ttacgatcaa
     3661 ttgttgtatc aaaatattta aaagcagcgg gagctccaag attcgtcaac gtaaataaat
     3721 gaataatatt ttctgcttgt tcacgtattg gtttgtctct atgtttgtta tatgcactaa
     3781 gaactttatc taaattggca tctgctaaaa taacacgctt agaaaattca ctgatttgct
     3841 caataatctc atctaaataa tgcttatgct gctccacaaa caattgtttt tgttcgttat
     3901 cttctggact acccttcaac ttttcataat gactagctaa atataaaaaa ttcacatatt
     3961 tgcttggcag agccagctca tttccttttt gtaattctcc ggcactagcc agcatccgtt
     4021 tacgaccgtt ttctaactca aaaagactat atttaggtag tttaatgatt aagtcttttt
     4081 taacttcctt atatccttta gcttctaaaa agtcaatcgg atttttttca aaggaacttc
     4141 tttccataat tgtgatccct agtaactctt taacggattt taacttcttc gatttccctt
     4201 tttccacctt agcaaccact aggactgaat aagctaccgt tggactatca aaaccaccat
     4261 atttttttgg atcccagtct tttttacgag caataagctt gtccgaattt ctttttggta
     4321 aaattgactc cttggagaat ccgcctgtct gtacttctgt tttcttgaca atattgactt
     4381 ggggcatgga caatactttg cgcactgtgg caaaatctcg ccctttatcc cagacaattt
     4441 ctccagtttc cccattagtt tcgattagag ggcgtttgcg aatctctcca tttgcaagtg
     4501 taatttctgt tttgaagaag ttcatgatat tagagtaaaa gaaatatttt gcggttgctt
     4561 tgcctatttc ttgctcagac ttagcaatca ttttacgaac atcataaact ttataatcac
     4621 catagacaaa ctccgattca agttttggat atttcttaat caaagcagtt ccaacgacgg
     4681 catttagata cgcatcatgg gcatgatggt aattgttaat ctcacgtact ttatagaatt
     4741 ggaaatcttt tcggaagtca gaaactaatt tagattttaa ggtaatcact ttaacctctc
     4801 gaataagttt atcattttca tcgtatttag tattcatgcg actatccaaa atttgtgcca
     4861 catgcttagt gatttggcga gtttcaacca attggcgttt gataaaacca gctttatcaa
     4921 gttcactcaa acctccacgt tcagctttcg ttaaattatc aaacttacgt tgagtgatta
     4981 acttggcgtt tagaagttgt ctccaatagt ttttcatctt tttgactact tcttcacttg
     5041 gaacgttatc cgatttacca cgatttttat cagaacgcgt taagacctta ttgtctattg
     5101 aatcgtcttt aaggaaactt tgtggaacaa tgtgatcgac atcataatca cttaaacgat
     5161 taatatctaa ttcttggtcc acatacatgt ctcttccatt ttggagataa tagagataga
     5221 gcttttcatt ttgcaattga gtattttcaa caggatgctc tttaagaatc tgacttccta
     5281 attctttgat accttcttcg attcgtttca tacgctctcg cgaatttttc tggccctttt
     5341 gagttgtctg attttcacgt gccatttcaa taacgatatt ttctggctta tgccgcccca
     5401 ttactttgac caattcatca acaactttta cagtctgtaa aatacctttt ttaatagcag
     5461 ggctaccagc taaatttgca atatgttcat gtaaactatc gccttgtcca gacacttgtg
     5521 ctttttgaat gtcttcttta aatgtcaaac tatcatcatg gatcagctgc ataaaattgc
     5581 gattggcaaa accatctgat ttcaaaaaat ctaatattgt tttgccagat tgcttatccc
     5641 taataccatt aatcaatttt cgagacaaac gtccccaacc agtataacgg cgacgtttaa
     5701 gctgtttcat caccttatca tcaaagaggt gagcatatgt tttaagtctt tcctcaatca
     5761 tctccctatc ttcaaataag gtcaatgtta aaacaatatc ctctaagata tcttcatttt
     5821 cttcattatc caaaaaatct ttatctttaa taatttttag caaatcatgg taggtaccta
     5881 atgaagcatt aaatctatct tcaactcctg aaatttcaac actatcaaaa cattctattt
     5941 ttttgaaata atcttctttt aattgcttaa cggttacttt tcgatttgtt ttgaagagta
     6001 aatcaacaat ggctttcttc tgttcacctg aaagaaatgc tggttttcgc attccttcag
     6061 taacatattt gacctttgtc aattcgttat aaaccgtaaa atactcataa agcaaactat
     6121 gttttggtag tactttttca tttggaagat ttttatcaaa gtttgtcatg cgttcaataa
     6181 atgattgagc tgaagcacct ttatcgacaa cttcttcaaa attccatggg gtaattgttt
     6241 cttcagactt ccgagtcatc catgcaaaac gactattgcc acgcgccaat ggaccaacat
     6301 aataaggaat tcgaaaagtc aagatttttt caatcttctc acgattgtct tttaaaaatg
     6361 gataaaagtc ttcttgtctt ctcaaaatag catgcagctc acccaagtga atttgatggg
     6421 gaatagagcc gttgtcaaag gtccgttgct tgcgcagcaa atcttcacga tttagtttca
     6481 ccaataattc ctcagtacca tccatttttt ctaaaattgg tttgataaat ttataaaatt
     6541 cttcttggct agctccccca tcaatataac ctgcatatcc gttttttgat tgatcaaaaa
     6601 agatttcttt atacttttct ggaagttgtt gtcgaactaa agcttttaaa agagtcaagt
     6661 cttgatgatg ttcatcgtag cgtttaatca ttgaagctga taggggagcc ttagttattt
     6721 cagtatttac tcttaggata tctgaaagta aaatagcatc tgataaattc ttagctgcca
     6781 aaaacaaatc agcatattga tctccaattt gcgccaataa attatctaaa tcatcatcgt
     6841 aagtatcttt tgaaagctgt aatttagcat cttctgccaa atcaaaattt gatttaaaat
     6901 taggggtcaa acccaatgac aaagcaatga gattcccaaa taagccattt ttcttctcac
     6961 cggggagctg agcaatgaga ttttctaatc gtcttgattt actcaatcgt gcagaaagaa
     7021 tcgctttagc atctactcca cttgcgttaa tagggttttc ttcaaataat tgattgtagg
     7081 tttgtaccaa ctggataaat agtttgtcca catcactatt atcaggattt aaatctccct
     7141 caatcaaaaa atgaccacga aacttaatca tatgcgctaa ggccaaatag attaagcgca
     7201 aatccgcttt atcagtagaa tctaccaatt tttttcgcag atgatagata gttggatatt
     7261 tctcatgata agcaacttca tctactatat ttccaaaaat aggatgacgt tcatgcttct
     7321 tgtcttcttc caccaaaaaa gactcttcaa gtcgatgaaa gaaactatca tctactttcg
     7381 ccatctcatt tgaaaaaatc tcctgtagat aacaaatacg attcttccga cgtgtatacc
     7441 ttctacgagc tgtccgtttg agacgagtcg cttccgctgt ctctccactg tcaaataaaa
     7501 gagcccctat aagatttttt ttgatactgt ggcggtctgt atttcccaga accttgaact
     7561 ttttagacgg aaccttatat tcatcagtga tcaccgccca tccgacgcta tttgtgccga
     7621 tatctaagcc tattgagtat ttcttatcca ttgattgtac ctcctggtca gtgcgtcctg
     7681 ctgatagcta gcatagtacc taggactgag ctagccgtaa actctagaag cggccgcgaa
     7741 ttcaactgca gtctagactc gagtaaggat ccagttcacc gacaaacaac agataaaacg
     7801 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     7861 tcgcatgggg agaccccaca ctaccatcgg cgctacggcg tttcacttct gagttcggca
     7921 tggggtcagg tgggaccacc gcgctactgc cgccaggcaa attctgtttt atcagaccgc
     7981 ttctgcgttc tgatttaatc tgtatcaggc tgaaaatctt ctctcatccg ccaaaacagc
     8041 caagctggag accgtttaaa ctcaatgatg atgatgatga tggtcgacgg cgctattcag
     8101 atcctcttct gagatgagtt tttgttcggg cccaagcttc aaaaaaagca ccgactcggt
     8161 gccacttttt caagttgata acggactagc cttattttaa cttgctattt ctagctctaa
     8221 aacggaagct ccctcgtgcg ctcgtgctca gtatctctat cactgatagg gatgtcaatc
     8281 tctatcactg atagggactc gagctatgct actccgtcaa gccgtcaatt gtctgattcg
     8341 ttaccaactc gaggacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg
     8401 cccggaagag agtcaattca gggtggtgaa tatgtctaga ttagataaaa gtaaagtgat
     8461 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
     8521 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
     8581 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
     8641 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
     8701 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
     8761 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
     8821 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
     8881 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
     8941 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
     9001 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt cttaatacca
     9061 ccgtcaaaaa aaacggcgct ttttagcgcc gtttttattt ttcaacctta agcttgaatt
     9121 cgttgacgaa ttctctagat atcgctcaat actgaccatt taaatcatac ctgacctcca
     9181 tagcagaaag tcaaaagcct ccgaccggag gcttttgact tgagggggat ccggcacatt
     9241 tccccgaaaa gtgccacctg catcgattta ttatgacaac ttgacggcta catcattcac
     9301 tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac
     9361 ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat
     9421 ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa
     9481 gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac
     9541 atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg
     9601 acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat
     9661 gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc
     9721 cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc
     9781 atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca
     9841 gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg
     9901 accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa
     9961 ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc
    10021 tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt
    10081 taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc
    10141 ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat
    10201 cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc
    10261 gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa
    10321 aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg
    10381 ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca
    10441 actctctact gtttctccat acccgttttt ttgggaattc gagctctaag gaggttataa
    10501 aaaatggata ttaatactga aactgagatc aagcaaaagc attcactaac cccctttcct
    10561 gttttcctaa tcagcccggc atttcgcggg cgatattttc acagctattt caggagttca
    10621 gccatgaacg cttattacat tcaggatcgt cttgaggctc agagctgggc gcgtcactac
    10681 cagcagctcg cccgtgaaga gaaagaggca gaactggcag acgacatgga aaaaggcctg
    10741 ccccagcacc tgtttgaatc gctatgcatc gatcatttgc aacgccacgg ggccagcaaa
    10801 aaatccatta cccgtgcgtt tgatgacgat gttgagtttc aggagcgcat ggcagaacac
    10861 atccggtaca tggttgaaac cattgctcac caccaggttg atattgattc agaggtataa
    10921 aacgaatgag tactgcactc gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg
    10981 attctgtcga cccacaggaa ctgatcacca ctcttcgcca gacggcattt aaaggtgatg
    11041 ccagcgatgc gcagttcatc gcattactga tcgttgccaa ccagtacggc cttaatccgt
    11101 ggacgaaaga aatttacgcc tttcctgata agcagaatgg catcgttccg gtggtgggcg
    11161 ttgatggctg gtcccgcatc atcaatgaaa accagcagtt tgatggcatg gactttgagc
    11221 aggacaatga atcctgtaca tgccggattt accgcaagga ccgtaatcat ccgatctgcg
    11281 ttaccgaatg gatggatgaa tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa
    11341 tcacggggcc gtggcagtcg catcccaaac ggatgttacg tcataaagcc atgattcagt
    11401 gtgcccgtct ggccttcgga tttgctggta tctatgacaa ggatgaagcc gagcgcattg
    11461 tcgaaaatac tgcatacact gcagaacgtc agccggaacg cgacatcact ccggttaacg
    11521 atgaaaccat gcaggagatt aacactctgc tgatcgccct ggataaaaca tgggatgacg
    11581 acttattgcc gctctgttcc cagatatttc gccgcgacat tcgtgcatcg tcagaactga
    11641 cacaggccga agcagtaaaa gctcttggat tcctgaaaca gaaagccgca gagcagaagg
    11701 tggcagcatg acaccggaca ttatcctgca gcgtaccggg atcgatgtga gagctgtcga
    11761 acagggggat gatgcgtggc acaaattacg gctcggcgtc atcaccgctt cagaagttca
    11821 caacgtgata gcaaaacccc gctccggaaa gaagtggcct gacatgaaaa tgtcctactt
    11881 ccacaccctg cttgctgagg tttgcaccgg tgtggctccg gaagttaacg ctaaagcact
    11941 ggcctgggga aaacagtacg agaacgacgc cagaaccctg tttgaattca cttccggcgt
    12001 gaatgttact gaatccccga tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc
    12061 cgatggttta tgcagtgacg gcaacggcct tgaactgaaa tgcccgttta cctcccggga
    12121 tttcatgaag ttccggctcg gtggtttcga ggccataaag tcagcttaca tggcccaggt
    12181 gcagtacagc atgtgggtga cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg
    12241 tatgaagcgt gaaggcctgc attatgtcgt gattgagcgg gatgaaaagt acatggcgag
    12301 ttttgacgag atcgtgccgg agttcatcga aaaaatggac gaggcactgg ctgaaattgg
    12361 ttttgtattt ggggagcaat ggcgatgacg catcctcacg ataatatccg ggtaggcgca
    12421 atcactttcg tctactccgt tacaaagcga ggctgggtat ttcccggcct ttctgttatc
    12481 cgaaatccac tgaaagcaca gcggctggct gaggagataa ataataaacg aggggctgta
    12541 tgcacaaagc atcttctgtt gagttaagaa cgagtatcga gatggcacat agccttgctc
    12601 aaattggaat caggtttgtg ccaataccag tagaaacaga cgaagaatcc atgggtatgg
    12661 acagttttcc ct
//
