LOCUS       Exported                4540 bp ds-DNA     circular SYN 11-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2018 from SnapGene 4.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="synthetic DNA construct"
     promoter        25..59
                     /label=J23119 promoter
     primer_bind     complement(38..79)
                     /label=NC-3R
     primer_bind     60..101
                     /label=NC-3F
     misc_RNA        80..155
                     /label=gRNA scaffold
     primer_bind     166..207
                     /label=NT-1F
     misc_feature    187..686
                     /label=NicCF
     primer_bind     666..707
                     /label=NT-2F
     misc_feature    687..1186
                     /label=NicCR
     primer_bind     1166..1207
                     /label=NT-3F
     misc_feature    complement(1213..1236)
                     /label=F24
     terminator      1243..1345
                     /label=T0
     gene            1589..2122
                     /label=acC3
     misc_feature    2198..2443
                     /label=oriT
     gene            3177..4019
                     /label=rep
     misc_feature    3771..3790
                     /label=2
     misc_feature    3809..3828
                     /label=1
     misc_feature    3954..3973
                     /label=3
     misc_feature    3979..3998
                     /label=4
     terminator      4435..4539
                     /label=T1
ORIGIN
        1 ttaattaatt gacaccatga attcttgaca gctagctcag tcctaggtat aatgctagca
       61 ggcagaaaat cgcaatcgtg ttttagagct agaaatagca agttaaaata aggctagtcc
      121 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttgaggagct cggtacccgg
      181 ggatccggca gctctaccgc atacaccttg gccttcaaac gctgggcggc ctccatggcg
      241 gcgttgaccg tgcgcgggtc ggagtagtgg ctcttgagca cggccacgct ctgcgtttcg
      301 tccactcgag acagcttcag tacgtgctcg aacatctggg tcagttgtgc attgctcacc
      361 ggcatcgggg ttctcctggg tggcgttgac aggggcgcac catggcagtg cctggtgctg
      421 catctcggtg caaaataata gtgtgtacac cctacttgtg cagatgaaaa cgcatcctgc
      481 cagcgagcgc aagtgatctg ttcaaacgtt acctatccac acattgacca gtatttacgg
      541 taacaatcaa ccggatttcg tttcgatcag ctgtttgaaa aatattttca gctggctctt
      601 ttcaaaggtc atttgtagcg tatacgctct aaatcgtcag gcggttgagc cgctggccac
      661 ctatcagaac aagaggagac atacccgcac cttcgtcgct ggtggtaacg tcagcgccaa
      721 tggcatccgc cagcattacc tgcgctatgg cggcaagggc catgcgctga tcctggtgcc
      781 gggcatcacc agcccagcga tcacctgggg ctttgtcgcc gagcgccttg ggcattattt
      841 cgacacctat gtgctggatg tgcgcgggcg tggcttgtct tcaagcggcc ccgacctgga
      901 ctacggcacc gacgcttgtg ctgcggatat tcccgccttt gccgccgcgc tgggcctgga
      961 tagctaccat ctgctcggtc actcgatggg ggcgcgtttt gccatccgcg cagccgccca
     1021 aggggcaccg ggactgcagc ggctggtgct ggtcgaccca ccggtgtccg ggccgggccg
     1081 ccgcgcctac ccgagcaagt tgccgtggta cgtcgattcg atccgccaag ccacggttgg
     1141 catgagcggc gatgacatgc gcgccttctg tgccacctgg agcgacctgc aggcatgcaa
     1201 gcttgcggcc gcgtcgtgac tgggaaaacc ctggcgacta gtcttggact cctgttgata
     1261 gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg
     1321 gcgtttttta ttggtgagaa tccaggggtc cccaataatt acgatttaaa tttgacataa
     1381 gcctgttcgg ttcgtaaact gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1441 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1501 tgtttttttg tacagcctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     1561 cgatgtttga tgttatggag cagcaacgat gttacgcagc agcaacgatg ttacgcagca
     1621 gggcagtcgc cctaaaacaa agttaggtgg ctcaagtatg ggcatcattc gcacatgtag
     1681 gctcggccct gaccaagtca aatccatgcg ggctgctctt gatcttttcg gtcgtgagtt
     1741 cggagacgta gccacctact cccaacatca gccggactcc gattacctcg ggaacttgct
     1801 ccgtagtaag acattcatcg cgcttgctgc cttcgaccaa gaagcggttg ttggcgctct
     1861 cgcggcttac gttctgccca agtttgagca gccgcgtagt gagatctata tctatgatct
     1921 cgcagtctcc ggagagcacc ggaggcaggg cattgccacc gcgctcatca atctcctcaa
     1981 gcatgaggcc aacgcgcttg gtgcttatgt gatctacgtg caagcagatt acggtgacga
     2041 tcccgcagtg gctctctata caaagttggg catacgggaa gaagtgatgc actttgatat
     2101 cgacccaagt accgccacct aacaattcgt tcaagccgag atcggcttcc cggccgcgga
     2161 gttgttcggt aaattggaca acggtccgcg cgttgtcctt ttccgctgca taaccctgct
     2221 tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat
     2281 tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga
     2341 taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc
     2401 tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaggccggc cgataatctc
     2461 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2521 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2581 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2641 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2701 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2761 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2821 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2881 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2941 acgcttcccg aagggagaaa ggcggacagg catccggtaa gcggcagggt cggaacagga
     3001 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3061 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3121 aaaaacgcca gcaacgcggc cgtgaaaggc aggccggtcc gtggtggcca cggcctctag
     3181 gccagatcca gcggcatctg ggttagtcga gcgcgggccg cttcccatgt ctcaccaggg
     3241 cgagcctgtt tcgcgatctc agcatctgaa atcttcccgg ccttgcgctt cgctggggcc
     3301 ttacccaccg ccttggcggg cttcttcggt ccaaaactga acaacagatg tgtgaccttg
     3361 cgcccggtct ttcgctgcgc ccactccacc tgtagcgggc tgtgctcgtt gatctgcgtc
     3421 acggctggat caagcactcg caacttgaag tccttgatcg agggataccg gccttccagt
     3481 tgaaaccact ttcgcagctg gtcaatttct atttcgcgct ggccgatgct gtcccattgc
     3541 atgagcagct cgtaaagcct gatcgcgtgg gtgctgtcca tcttggccac gtcagccaag
     3601 gcgtatttgg tgaactgttt ggtgagttcc gtcaggtacg gcagcatgtc tttggtgaac
     3661 ctgagttcta cacggccctc accctcccgg tagatgattg tttgcaccca gccggtaatc
     3721 atcacactcg gtcttttccc cttgccattg ggctcttggg ttaaccggac ttcccgccgt
     3781 ttcaggcgca gggccgcttc tttgagctgg ttgtaggaag attcgatagg gacacccgcc
     3841 atcgtcgcta tgtcctccgc cgtcactgaa tacatcactt catcggtgac aggctcgctc
     3901 ctcttcacct ggctaataca ggccagaacg atccgctgtt cctgaacact gaggcgatac
     3961 gcggcctcga ccagggcatt gcttttgtaa accattgggg gtgaggccac gttcgacatt
     4021 ccttgtgtat aaggggacac tgtatctgcg tcccacaata caacaaatcc gtccctttac
     4081 aacaacaaat ccgtcccttc ttaacaacaa atccgtccct taatggcaac aaatccgtcc
     4141 ctttttaaac tctacaggcc acggattacg tggcctgtag acgtcctaaa aggtttaaaa
     4201 gggaaaagga agaaaagggt ggaaacgcaa aaaacgcacc actacgtggc cccgttgggg
     4261 ccgcatttgt gcccctgaag gggcggggga ggcgtctggg caatccccgt tttaccagtc
     4321 ccctatcgcc gcctgagagg gcgcaggaag cgagtaatca gggtatcgag gcggattcac
     4381 ccttggcgtc caaccagcgg caccagcggc gcctgagagg ggcgcgccca gctgtctagg
     4441 gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     4501 cccagtcttt cgactgagcc tttcgtttta tttgatgcct
//
