LOCUS       AAV-nEF-dC   7711 bp    DNA   circular             7-JAN-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|-12105707|
COMMENT     VNTDBDATE|-12105707|
COMMENT     LSOWNER|
COMMENT     VNTNAME|AAV-nEF-dCas9|
COMMENT     VNTAUTHORNAME|Ken|
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BASE COUNT     2086 a      1922 c      1945 g      1758 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggcctct agaaaggatc tgcgatcgct ccggtgcccg 
      181 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa 
      241 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg 
      301 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa 
      361 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat 
      421 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt 
      481 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa 
      541 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg 
      601 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt 
      661 tctgcgccgt tacagatcca agctgtgacc ggcgcctaca ccggtgccac catgtaccca 
      721 tacgatgttc cagattacgc ttcgccgaag aaaaagcgca aggtcgaagc gtccgacaag 
      781 aagtactcca ttgggctcgc tatcggcaca aacagcgtcg gctgggccgt cattacggac 
      841 gagtacaagg tgccgagcaa aaaattcaaa gttctgggca ataccgatcg ccacagcata 
      901 aagaagaacc tcattggcgc cctcctgttc gactccgggg agacggccga agccacgcgg 
      961 ctcaaaagaa cagcacggcg cagatatacc cgcagaaaga atcggatctg ctacctgcag 
     1021 gagatcttta gtaatgagat ggctaaggtg gatgactctt tcttccatag gctggaggag 
     1081 tcctttttgg tggaggagga taaaaagcac gagcgccacc caatctttgg caatatcgtg 
     1141 gacgaggtgg cgtaccatga aaagtaccca accatatatc atctgaggaa gaagcttgta 
     1201 gacagtactg ataaggctga cttgcggttg atctatctcg cgctggcgca tatgatcaaa 
     1261 tttcggggac acttcctcat cgagggggac ctgaacccag acaacagcga tgtcgacaaa 
     1321 ctctttatcc aactggttca gacttacaat cagcttttcg aagagaaccc gatcaacgca 
     1381 tccggagttg acgccaaagc aatcctgagc gctaggctgt ccaaatcccg gcggctcgaa 
     1441 aacctcatcg cacagctccc tggggagaag aagaacggcc tgtttggtaa tcttatcgcc 
     1501 ctgtcactcg ggctgacccc caactttaaa tctaacttcg acctggccga agatgccaag 
     1561 cttcaactga gcaaagacac ctacgatgat gatctcgaca atctgctggc ccagatcggc 
     1621 gaccagtacg cagacctttt tttggcggca aagaacctgt cagacgccat tctgctgagt 
     1681 gatattctgc gagtgaacac ggagatcacc aaagctccgc tgagcgctag tatgatcaag 
     1741 cgctatgatg agcaccacca agacttgact ttgctgaagg cccttgtcag acagcaactg 
     1801 cctgagaagt acaaggaaat tttcttcgat cagtctaaaa atggctacgc cggatacatt 
     1861 gacggcggag caagccagga ggaattttac aaatttatta agcccatctt ggaaaaaatg 
     1921 gacggcaccg aggagctgct ggtaaagctt aacagagaag atctgttgcg caaacagcgc 
     1981 actttcgaca atggaagcat cccccaccag attcacctgg gcgaactgca cgctatcctc 
     2041 aggcggcaag aggatttcta cccctttttg aaagataaca gggaaaagat tgagaaaatc 
     2101 ctcacatttc ggatacccta ctatgtaggc cccctcgccc ggggaaattc cagattcgcg 
     2161 tggatgactc gcaaatcaga agagaccatc actccctgga acttcgagga agtcgtggat 
     2221 aagggggcct ctgcccagtc cttcatcgaa aggatgacta actttgataa aaatctgcct 
     2281 aacgaaaagg tgcttcctaa acactctctg ctgtacgagt acttcacagt ttataacgag 
     2341 ctcaccaagg tcaaatacgt cacagaaggg atgagaaagc cagcattcct gtctggagag 
     2401 cagaagaaag ctatcgtgga cctcctcttc aagacgaacc ggaaagttac cgtgaaacag 
     2461 ctcaaagaag actatttcaa aaagattgaa tgtttcgact ctgttgaaat cagcggagtg 
     2521 gaggatcgct tcaacgcatc cctgggaacg tatcacgatc tcctgaaaat cattaaagac 
     2581 aaggacttcc tggacaatga ggagaacgag gacattcttg aggacattgt cctcaccctt 
     2641 acgttgtttg aagataggga gatgattgaa gaacgcttga aaacttacgc tcatctcttc 
     2701 gacgacaaag tcatgaaaca gctcaagagg cgccgatata caggatgggg gcggctgtca 
     2761 agaaaactga tcaatgggat ccgagacaag cagagtggaa agacaatcct ggattttctt 
     2821 aagtccgatg gatttgccaa ccggaacttc atgcagttga tccatgatga ctctctcacc 
     2881 tttaaggagg acatccagaa agcacaagtt tctggccagg gggacagtct tcacgagcac 
     2941 atcgctaatc ttgcaggtag cccagctatc aaaaagggaa tactgcagac cgttaaggtc 
     3001 gtggatgaac tcgtcaaagt aatgggaagg cataagcccg agaatatcgt tatcgagatg 
     3061 gcccgagaga accaaactac ccagaaggga cagaagaaca gtagggaaag gatgaagagg 
     3121 attgaagagg gtataaaaga actggggtcc caaatcctta aggaacaccc agttgaaaac 
     3181 acccagcttc agaatgagaa gctctacctg tactacctgc agaacggcag ggacatgtac 
     3241 gtggatcagg aactggacat caatcggctc tccgactacg acgtggctgc tatcgtgccc 
     3301 cagtcttttc tcaaagatga ttctattgat aataaagtgt tgacaagatc cgataaagct 
     3361 agagggaaga gtgataacgt cccctcagaa gaagttgtca agaaaatgaa aaattattgg 
     3421 cggcagctgc tgaacgccaa actgatcaca caacggaagt tcgataatct gactaaggct 
     3481 gaacgaggtg gcctgtctga gttggataaa gccggcttca tcaaaaggca gcttgttgag 
     3541 acacgccaga tcaccaagca cgtggcccaa attctcgatt cacgcatgaa caccaagtac 
     3601 gatgaaaatg acaaactgat tcgagaggtg aaagttatta ctctgaagtc taagctggtc 
     3661 tcagatttca gaaaggactt tcagttttat aaggtgagag agatcaacaa ttaccaccat 
     3721 gcgcatgatg cctacctgaa tgcagtggta ggcactgcac ttatcaaaaa atatcccaag 
     3781 cttgaatctg aatttgttta cggagactat aaagtgtacg atgttaggaa aatgatcgca 
     3841 aagtctgagc aggaaatagg caaggccacc gctaagtact tcttttacag caatattatg 
     3901 aattttttca agaccgagat tacactggcc aatggagaga ttcggaagcg accacttatc 
     3961 gaaacaaacg gagaaacagg agaaatcgtg tgggacaagg gtagggattt cgcgacagtc 
     4021 cggaaggtcc tgtccatgcc gcaggtgaac atcgttaaaa agaccgaagt acagaccgga 
     4081 ggcttctcca aggaaagtat cctcccgaaa aggaacagcg acaagctgat cgcacgcaaa 
     4141 aaagattggg accccaagaa atacggcgga ttcgattctc ctacagtcgc ttacagtgta 
     4201 ctggttgtgg ccaaagtgga gaaagggaag tctaaaaaac tcaaaagcgt caaggaactg 
     4261 ctgggcatca caatcatgga gcgatcaagc ttcgaaaaaa accccatcga ctttctcgag 
     4321 gcgaaaggat ataaagaggt caaaaaagac ctcatcatta agcttcccaa gtactctctc 
     4381 tttgagcttg aaaacggccg gaaacgaatg ctcgctagtg cgggcgagct gcagaaaggt 
     4441 aacgagctgg cactgccctc taaatacgtt aatttcttgt atctggccag ccactatgaa 
     4501 aagctcaaag ggtctcccga agataatgag cagaagcagc tgttcgtgga acaacacaaa 
     4561 cactaccttg atgagatcat cgagcaaata agcgaattct ccaaaagagt gatcctcgcc 
     4621 gacgctaacc tcgataaggt gctttctgct tacaataagc acagggataa gcccatcagg 
     4681 gagcaggcag aaaacattat ccacttgttt actctgacca acttgggcgc gcctgcagcc 
     4741 ttcaagtact tcgacaccac catagacaga aagcggtaca cctctacaaa ggaggtcctg 
     4801 gacgccacac tgattcatca gtcaattacg gggctctatg aaacaagaat cgacctctct 
     4861 cagctcggtg gagacagccc caagaagaag agaaaggtgg aggccagcta agaattcaat 
     4921 aaaagatctt tattttcatt agatctgtgt gttggttttt tgtgtgcggc cgcaggaacc 
     4981 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg 
     5041 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg 
     5101 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc 
     5161 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg 
     5221 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 
     5281 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 
     5341 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 
     5401 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 
     5461 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 
     5521 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 
     5581 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca 
     5641 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga 
     5701 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 
     5761 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 
     5821 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata 
     5881 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 
     5941 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 
     6001 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 
     6061 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 
     6121 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 
     6181 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 
     6241 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 
     6301 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 
     6361 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 
     6421 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 
     6481 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 
     6541 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 
     6601 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 
     6661 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 
     6721 gataaatctg gagccggtga gcgtggaagc cgcggtatca ttgcagcact ggggccagat 
     6781 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 
     6841 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 
     6901 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 
     6961 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 
     7021 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 
     7081 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 
     7141 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 
     7201 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 
     7261 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 
     7321 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 
     7381 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 
     7441 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 
     7501 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 
     7561 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 
     7621 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 
     7681 ctggcctttt gctggccttt tgctcacatg t 
//
