LOCUS       Exported               12129 bp ds-DNA     circular SYN 08-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 12129)
  AUTHORS   Raymond Bourdeau
  TITLE     Direct Submission
  JOURNAL   Exported Jan 8, 2018 from SnapGene 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12129
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(560..1375)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1497..2085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2271..2413
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2515..2706)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3515..4597)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(4598..4675)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        4984..5002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    5059..5355
                     /label=copy1
     CDS             5129..5344
                     /codon_start=1
                     /label=GvpA1
                     /translation="MAVEKTNSSSSLAEVIDRILDKGIVIDAWVRVSLVGIELLAIEAR
                     IVIASVETYLKYAEAVGLTQSAAVPA"
     misc_feature    5356..5379
                     /label=copy2_unique
     misc_feature    5380..5676
                     /label=copy2
     CDS             5450..5665
                     /codon_start=1
                     /label=GvpA2
                     /translation="MAVEKTNSSSSLAEVIDRILDKGIVIDAWVRVSLVGIELLAIEAR
                     IVIASVETYLKYAEAVGLTQSAAVPA"
     CDS             5898..6479
                     /codon_start=1
                     /label=GvpC
                     /note="Ana GvpC"
                     /translation="MISLMAKIRQEHQSIAEKVAELSLETREFLSVTTAKRQEQAEKQA
                     QELQAFYKDLQETSQQFLSETAQARIAQAEKQAQELLAFHKELQETSQQFLSATAQARI
                     AQAEKQAQELLAFYQEVRETSQQFLSATAQARIAQAEKQAQELLAFHKELQETSQQFLS
                     ATADARTAQAKEQKESLLKFRQDLFVSIFG"
     misc_feature    5952..6050
                     /label=repeat1
     misc_feature    6051..6149
                     /label=repeat2
     misc_feature    6150..6248
                     /label=repeat3
     misc_feature    6249..6347
                     /label=repeat4
     misc_feature    6348..6446
                     /label=repeat5
     misc_feature    6495..6503
                     /label=RBS
     CDS             6510..6776
                     /codon_start=1
                     /label=GvpR
                     /translation="MEIKKIMQAVNDFFGEHVAPPHKITSVEATEDEGWRVIVEVIEER
                     EYMKKYAKDEMLGTYECFVNKEKEVISFKRLDVRYRSAIGIEA"
     CDS             6805..7731
                     /codon_start=1
                     /label=GvpN
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     CDS             7746..8513
                     /codon_start=1
                     /label=GvpF
                     /translation="MSETNETGIYIFSAIQTDKDEEFGAVEVEGTKAETFLIRYKDAAM
                     VAAEVPMKIYHPNRQNLLMHQNAVAAIMDKNDTVIPISFGNVFKSKEDVKVLLENLYPQ
                     FEKLFPAIKGKIEVGLKVIGKKEWLEKKVNENPELEKVSASVKGKSEAAGYYERIQLGG
                     MAQKMFTSLQKEVKTDVFSPLEEAAEAAKANEPTGETMLLNASFLINREDEAKFDEKVN
                     EAHENWKDKADFHYSGPWPAYNFVNIRLKVEEK"
     CDS             8515..8781
                     /codon_start=1
                     /label=GvpG
                     /translation="VLHKLVTAPINLVVKIGEKVQEEADKQLYDLPTIQQKLIQLQMMF
                     ELGEIPEEAFQEKEDELLMRYEIAKRREIEQWEELTQKRNEES"
     CDS             8782..9591
                     /codon_start=1
                     /label=GvpL
                     /translation="MGELLYLYGLIPTKEAAAIEPFPSYKGFDGEHSLYPIAFDQVTAV
                     VSKLDADTYSEKVIQEKMEQDMSWLQEKAFHHHETVAALYEEFTIIPLKFCTIYKGEES
                     LQAAIEINKEKIENSLTLLQGNEEWNVKIYCDDTELKKGISETNESVKAKKQEISHLSP
                     GRQFFEKKKIDQLIEKELELHKNKVCEEIHDKLKELSLYDSVKKNWSKDVTGAAEQMAW
                     NSVFLLPSLQITKFVNEIEELQQRLENKGWKFEVTGPWPPYHFSSFA"
     misc_feature    9371
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     CDS             9609..9896
                     /codon_start=1
                     /label=GvpS
                     /translation="MSLKQSMENKDIALIDILDVILDKGVAIKGDLIISIAGVDLVYLD
                     LRVLISSVETLVQAKEGNHKPITSEQFDKQKEELMDATGQPSKWTNPLGS"
     CDS             9850..10134
                     /codon_start=1
                     /label=GvpK
                     /translation="MQPVSQANGRIHLDPDQAEQGLAQLVMTVIELLRQIVERHAMRRV
                     EGGTLTDEQIENLGIALMNLEEKMDELKEVFGLDAEDLNIDLGPLGSLL"
     CDS             10156..10458
                     /codon_start=1
                     /label=GvpJ
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     CDS             10495..11373
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     CDS             11397..11813
                     /codon_start=1
                     /label=GvpU
                     /translation="MSTGPSFSTKDNTLEYFVKASNKHGFSLDISLNVNGAVISGTMIS
                     AKEYFDYLSETFEEGSEVAQALSEQFSLASEASESNGEAEAHFIHLKNTKIYCGDSKST
                     PSKGKIFWRGKIAEVDGFFLGKISDAKSTSKKSS"
     terminator      12057..12104
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattccaag cttcgccagg
     5041 gttttcccag tcgagctcat gactcatatt tatagttatt ctcctcattt cccaagtttc
     5101 cgacaacaaa taacaaggaa ttaaaaaaat ggcagttgaa aaaaccaatt cttcctccag
     5161 cttggcagaa gttattgata gaatcctcga caaaggtatc gtaattgacg cttgggttcg
     5221 tgtttcctta gttggtatcg aactactagc aattgaagct cggatcgtta tcgcttccgt
     5281 tgaaacctac ttgaagtatg ctgaagcagt tggtttgacc caatcagcag cagtacctgc
     5341 ttaatttaat taagcaagtt accaagtagg agaataacca tgactcatat ttatagttat
     5401 tctcctcatt tcccaagttt ccgacaacaa ataacaagga attaaaaaaa tggcagttga
     5461 aaaaaccaat tcttcctcca gcttggcaga agttattgat agaatcctcg acaaaggtat
     5521 cgtaattgac gcttgggttc gtgtttcctt agttggtatc gaactactag caattgaagc
     5581 tcggatcgtt atcgcttccg ttgaaaccta cttgaagtat gctgaagcag ttggtttgac
     5641 ccaatcagca gcagtacctg cttaatttaa ttaagctcca taatatcagg attaattaat
     5701 cctgatattg gttctgcttt ttttaattat ctagatattt ggaaatcaac gcttctcaaa
     5761 ggatattaca aaaaagtacg aatccttata atgtagtacc tggagaagca atttctgtta
     5821 ggtctgggag ctcccatggg atcgttatcc cgcggccaag cttgctagcg ctaatttact
     5881 attttttgga gaacttcatg atttctttaa tggcaaaaat ccggcaagaa catcagtcaa
     5941 tagcagagaa agtggctgaa ctatctcttg agaccagaga attcttgtcc gtcacgacag
     6001 cgaaaagaca agagcaagct gaaaaacaag ctcaagaact gcaagcattc tacaaggatc
     6061 ttcaggaaac aagtcagcag tttttatcag aaacagccca agccagaatt gctcaagctg
     6121 aaaaacaagc tcaagaactg ttagcattcc acaaagaact tcaagaaaca agtcagcagt
     6181 ttttatcagc aacagcccaa gccagaattg ctcaagctga aaaacaagcg caagaactgt
     6241 tagcatttta tcaagaagtt cgggaaacaa gtcagcagtt tttatcagca acagcccaag
     6301 caagaattgc tcaagctgaa aaacaagctc aagaactgtt agcattccac aaagaacttc
     6361 aagaaacaag tcagcagttt ttatcagcaa cagccgacgc aagaactgct caagctaagg
     6421 aacagaagga atctctcctg aaattccgtc aggatttgtt tgtgagtatc tttggttaat
     6481 aaagatctaa ctattggagg ctactaaaaa tggaaattaa aaaaattatg caagccgtga
     6541 acgacttttt cggtgaacac gtagctcctc ctcataaaat tacctcggtg gaagctactg
     6601 aagatgaagg ttggagagtt attgttgaag tcattgaaga acgagaatat atgaaaaaat
     6661 acgccaaaga tgaaatgctc ggaacgtacg agtgctttgt aaataaagaa aaagaagtca
     6721 tttcattcaa acgactcgac gtcagatata gaagcgccat tggcattgaa gcataaacga
     6781 taagatggca ggaggaacgt aaaaatgacc gtcttaacag acaaaaggaa aaaaggcagt
     6841 ggagctttta tacaagatga cgagacaaaa gaggttcttt caagagcgct gagctattta
     6901 aaatccggct attccattca ttttacaggt cctgccggcg gaggcaaaac ctctttagcg
     6961 cgagcgcttg ctaaaaagag aaagcgtcct gtaatgctga tgcacgggaa tcacgagctc
     7021 aacaacaaag atttaattgg cgattttacg ggatacacga gcaaaaaagt aatcgaccag
     7081 tacgttcgtt ctgtctataa aaaagatgaa caggtgagtg aaaactggca ggatggccga
     7141 ttgcttgaag ctgtaaaaaa tggctatacg ctgatttacg acgaatttac tcgttctaag
     7201 cctgcgacga ataatatctt tctatcgata ttagaagaag gcgtgctgcc gctgtatgga
     7261 gtaaaaatga ccgatccttt tgtgcgcgtg catcccgatt tccgcgtcat cttcacaagc
     7321 aatccagctg agtatgccgg cgtatatgat acgcaagatg cgcttctcga caggttaatt
     7381 accatgttta ttgattataa agacatcgac agagagacag cgattttaac ggagaaaacg
     7441 gacgtagaag aagatgaagc gcgcacaatt gtaacgctcg tagcaaacgt gcgaaaccgc
     7501 tctggagacg aaaacagcag cggacttagc ctgcgggctt cgcttatgat cgctaccctt
     7561 gccacgcagc aagacattcc tatcgatgga agtgacgaag attttcaaac gttatgtatc
     7621 gatattttgc atcatccgct taccaaatgt ttggatgaag aaaatgcaaa aagcaaagcc
     7681 gaaaaaatca ttttagaaga atgtaagaat atagacactg aagaaaagta aaggagcttg
     7741 aaaacatgag tgaaacaaac gaaacaggta tttatatttt tagcgccatt caaacggata
     7801 aagacgaaga atttggcgcc gtggaagtag aaggaacaaa agctgaaaca tttttgattc
     7861 gctacaaaga cgcggctatg gtagcagctg aagtaccgat gaaaatttat catcctaatc
     7921 gccaaaattt attaatgcat caaaacgcag tagcagcgat tatggacaag aacgatacgg
     7981 ttattccaat cagctttggg aatgtattca aatcaaaaga agacgtaaaa gttcttttgg
     8041 aaaaccttta tccgcagttt gaaaagctgt ttccagcgat caaaggaaaa attgaagtcg
     8101 gtttaaaagt aattgggaaa aaagaatggc ttgagaaaaa agtaaacgaa aatcctgaac
     8161 ttgagaaagt atcagcatcc gtaaaaggaa aatcagaagc agccggttat tatgagcgta
     8221 ttcaacttgg aggaatggct caaaagatgt ttacttccct gcaaaaagaa gtcaagacag
     8281 atgtgttttc tccgcttgaa gaagcagcgg aagcagcaaa agcaaatgag ccaacgggcg
     8341 aaacgatgct tttaaacgcg tctttcttaa ttaaccgaga agatgaagcg aagtttgatg
     8401 aaaaagtaaa tgaagcgcat gaaaactgga aagacaaagc cgattttcat tacagcggtc
     8461 cttggcctgc ttataatttt gtgaacattc gcctaaaagt agaagagaaa taacgtgctt
     8521 cacaaattag taaccgcacc cattaacctt gtagtgaaaa tcggcgaaaa agtacaggaa
     8581 gaagctgata aacagctata tgaccttccg acgattcagc aaaagctcat tcagcttcaa
     8641 atgatgtttg agcttggtga aattccagaa gaagcgtttc aagaaaaaga agatgaattg
     8701 ttaatgaggt acgaaattgc gaaacgcaga gaaattgaac aatgggaaga gctaacacaa
     8761 aaaagaaatg aggaatccta gatgggagaa ttactgtatt tatacggttt aattccaaca
     8821 aaagaagcag cagccataga gccgtttcca tcttataagg ggtttgacgg agaacattca
     8881 ctgtacccaa ttgcgtttga tcaggtgacg gctgtagttt ctaagctgga tgctgacacc
     8941 tattcagaaa aagtgattca agaaaaaatg gagcaggata tgagctggct gcaggaaaaa
     9001 gcatttcatc atcacgaaac ggtagccgct ttgtacgaag aatttacgat cattccatta
     9061 aaattttgca ccatttataa aggtgaagaa agtctgcagg cagctattga gattaacaaa
     9121 gaaaagatag agaattcact gacgctgctt caaggaaatg aagagtggaa tgtgaaaatt
     9181 tactgtgatg atacagagct taaaaaagga atcagcgaaa cgaatgaaag cgtgaaagcg
     9241 aaaaaacaag aaattagtca cttatcacca ggaagacagt tttttgaaaa gaaaaaaata
     9301 gatcagctga ttgaaaaaga attagagctt cacaaaaaca aagtgtgtga agagatacat
     9361 gacaagctaa aagaattatc gctttatgac tctgttaaaa agaattggag caaagacgta
     9421 actggcgcag ctgaacagat ggcgtggaac agcgtgtttc ttctcccgtc tctgcagatt
     9481 actaagttcg taaacgaaat agaagagctt cagcaaaggc ttgaaaataa aggctggaag
     9541 tttgaagtga cgggaccatg gccgccctat catttctcga gctttgcgta aagtgaggaa
     9601 ttaacattat gtctcttaaa caatccatgg agaataaaga tattgctctt attgatattt
     9661 tagatgtcat tttagataaa ggagtcgcca ttaaaggaga cttaatcatt tccatagctg
     9721 gcgtcgattt agtgtatttg gatttgcggg tgcttatttc ttcggttgaa acgcttgtgc
     9781 aagcaaaaga aggaaatcac aaaccaatca cttctgaaca atttgataaa caaaaggagg
     9841 aattaatgga tgcaaccggt cagccaagca aatggacgaa tccacttgga tcctgatcaa
     9901 gctgaacaag gcttagcgca gcttgtgatg acagttattg agctattgag gcaaatcgtt
     9961 gaacgtcatg ccatgaggcg ggtggagggt ggaacgttga cggacgaaca aattgaaaac
    10021 ttaggaattg cactaatgaa cttagaagaa aaaatggacg agttgaaaga ggtgttcggt
    10081 ctggatgcag aagatttaaa tattgatctt ggaccgctag gcagcctgct ttaagcggtc
    10141 agtaggagga acagtatggc agtcgaacat aatatgcagt caagtacgat tgtagatgtg
    10201 ctcgaaaaga ttttggataa aggagtcgtt atagcggggg acatcaccgt aggaattgca
    10261 gatgtcgagc tattaacgat aaagatccgc ttgattgtgg cttcggttga taaggcaaaa
    10321 gaaatcggca tggactggtg ggaaaatgat ccgtatctca gttcaaaagg agccaataac
    10381 aaagcgctcg aagaagaaaa taaaatgctg catgagcggt taaaaacgct tgaagaaaaa
    10441 atagaaacga aacgttaaaa actgtacgct acttaaaaaa tggagggatt tacaatggca
    10501 actgaaacaa aattagataa cacacaggca gaaaacaagg aaaataaaaa tgcggaaaac
    10561 ggttcaaaag aaaagaacgg ttcaaaagca agcaaaacaa caagcagcgg gccaatcaaa
    10621 cgagcggtag caggaggcat catcggtgca acgattggat atgtatcgac tcctgaaaat
    10681 cgaaaaagtc tccttgaccg cattgataca gacgaattaa aaagcaaagc atctgattta
    10741 ggaacaaagg taaaagaaaa atcaaaaagc agcgtggcca gcctgaaaac atctgcggga
    10801 agcttgttta aaaaagataa agataaatca aaagatgatg aagaaaacgt aaattcttct
    10861 agtagcgaaa cagaagacga taacgttcaa gagtacgacg agttaaaaga agaaaatcaa
    10921 actcttcaag atcgcttatc acagcttgaa gaaaaaatga acatgcttgt tgagcttagc
    10981 ctcaataaaa atcaagacga agaagcggaa gatacagatt ccgacgaaga agagaacgat
    11041 gagaacgatg aaaacgatga aaacgagcag gacgatgaaa acgaagaaga aacatctaag
    11101 ccacgtaaaa aggataaaaa agaagctgag gaagaagaaa gtgaaagtga cgaagacagc
    11161 gaggaagaag aggaagattc tcgctcaaac aaaaaaaata aaaaagtaaa aacagaagaa
    11221 gaagacgaag atgaaagcga agaagaaaaa aaggaagcga aaccaaaaaa gtcaacagct
    11281 aaaaaatcaa aaaatacaaa agcaaagaaa aacacggacg aagaagatga tgaagcaaca
    11341 tctctttcta gtgaagacga tacaacagcc taagacgtaa aggaggaaag aaagacatga
    11401 gtacaggccc ttctttttca actaaagaca atacgcttga atactttgtg aaagcttcta
    11461 ataaacacgg cttttcactt gatatttcat taaatgtaaa cggcgctgtg atttccggta
    11521 ccatgatttc agcaaaagaa tactttgatt acttaagcga aacgtttgaa gaaggcagtg
    11581 aagtggctca ggcgctaagc gaacaattct ctttagcaag cgaagcgagc gaatcaaacg
    11641 gagaagcaga agcccatttt attcatttga aaaatacaaa gatttactgt ggagacagta
    11701 aatctactcc ttctaaaggc aaaatctttt ggagagggaa aatagcagaa gtagacgggt
    11761 ttttcttagg aaagatttct gatgcaaaat caacgagtaa aaagagttca taaaaaacgg
    11821 cggggtgatt gccccgccgt tttttagtga tgtgatgaga tgtgcagctt cttttttcga
    11881 tacatgagcg gagtcatctt catttctttt cgaaactgat taataaaata gcttgtacta
    11941 ttaaacccta cttgataggc gacttccgtc acatttgctt ctgcttgctg caggatccgg
    12001 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
    12061 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
    12121 tatccggat
//
