LOCUS       [1GibAss]_CROPseq-EFS- 13350 bp ds-DNA     circular     08-JAN-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    9183..9217
                     /label="Gib Assemb 3` overlap"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    5757..5804
                     /label="NLS"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(9267..9293)
                     /label="CROPseq_libQC_i7:1 overlap"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     origin          11673..12292
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1653..5756
                     /label="hSpCas9"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     feature         9923..10071
                     /label="lacZ_a"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     feature         1115..1130
                     /label="cPPT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     origin          9709..9807
                     /label="SV40_origin"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     feature         9311..9491
                     /label="truncHIV-1_3_LTR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     gene            5895..6293
                     /label="blast"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    9176..9181
                     /label="BsmBI recog site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        7062..7294
                     /label="hU6_promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7242..7255
                     /label="GeCKO: extra adapter added on with PCR amplification"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        complement(9914..9930)
                     /label="M13_forward20_primer"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     gene            10658..11518
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        12665..12683
                     /label="M13_reverse_primer"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(9923..9945)
                     /label="M13_pUC_fwd_primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1647..1652
                     /label="Kozak"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     origin          10089..10395
                     /label="f1_origin"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    7303..9182
                     /label="filler (to be replaced with sgRNA after digest with BsmBI)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    7280..7302
                     /label="illumina cropseq_libQC_i5_s1 overlap"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    9226..9242
                     /label="GeCKO: extra adapter added during PCR amplification"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    9183..9264
                     /label="sgRNA scaffold"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     feature         9311..9491
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          7224..7243
                     /label="LKO1_5_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    7284..7302
                     /label="Gib Assemb 5` overlap"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     feature         12973..13153
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          9660..9679
                     /label="EBV_rev_primer"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    7242..7255
                     /label="GeCKO: extra adapter added on with PCR amplification"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    9183..9225
                     /label="GeCKO sgRNA library flanking sequence"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     feature         6952..6973
                     /label="U3PPT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     feature         12973..13153
                     /label="truncHIV-1_3_LTR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    7280..7301
                     /label="NGS-Lib-KO-Fwd-1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(9886..9904)
                     /label="T7_promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1374..1629
                     /label="EFS (EF1a short promoter)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     feature         5805..5828
                     /label="FLAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     regulatory      468..701
                     /label="RRE"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    7303..7308
                     /label="BsmBI recog site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          12644..12661
                     /label="M13_pUC_rev_primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        10588..10616
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      9572..9691
                     /label="SV40_PA_terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    7256..7301
                     /label="GeCKO sgRNA library flanking sequence"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     feature         6311..6886
                     /label="WPRE"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        12700..12719
                     /label="T3_promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5829..5894
                     /label="P2A"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     feature         13264..13308
                     /label="HIV-1_psi_pack"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          9792..9811
                     /label="SV40pro_F_primer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        12601..12630
                     /label="lac_promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     feature         6952..6967
                     /label="cPPT"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
       61 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
      121 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
      181 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
      241 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
      301 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
      361 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
      421 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
      481 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
      541 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
      601 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
      661 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
      721 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
      781 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
      841 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
      901 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
      961 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     1021 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagaat
     1081 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     1141 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     1201 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     1261 ttggcgccgg ctcgaggggg cccgggtgca aagatggata aagttttaaa cagagaggaa
     1321 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccgTAGGTCT
     1381 TGAAAGGAGT GGGAATTGGC TCCGGTGCCC GTCAGTGGGC AGAGCGCACA TCGCCCACAG
     1441 TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGATCCGG TGCCTAGAGA AGGTGGCGCG
     1501 GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG GGTGGGGGAG
     1561 AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG TTTGCCGCCA
     1621 GAACACAGGA CCGGTTCTAG AGCGCTGCCA CCATGGACAA GAAGTACAGC ATCGGCCTGG
     1681 ACATCGGCAC CAACTCTGTG GGCTGGGCCG TGATCACCGA CGAGTACAAG GTGCCCAGCA
     1741 AGAAATTCAA GGTGCTGGGC AACACCGACC GGCACAGCAT CAAGAAGAAC CTGATCGGAG
     1801 CCCTGCTGTT CGACAGCGGC GAAACAGCCG AGGCCACCCG GCTGAAGAGA ACCGCCAGAA
     1861 GAAGATACAC CAGACGGAAG AACCGGATCT GCTATCTGCA AGAGATCTTC AGCAACGAGA
     1921 TGGCCAAGGT GGACGACAGC TTCTTCCACA GACTGGAAGA GTCCTTCCTG GTGGAAGAGG
     1981 ATAAGAAGCA CGAGCGGCAC CCCATCTTCG GCAACATCGT GGACGAGGTG GCCTACCACG
     2041 AGAAGTACCC CACCATCTAC CACCTGAGAA AGAAACTGGT GGACAGCACC GACAAGGCCG
     2101 ACCTGCGGCT GATCTATCTG GCCCTGGCCC ACATGATCAA GTTCCGGGGC CACTTCCTGA
     2161 TCGAGGGCGA CCTGAACCCC GACAACAGCG ACGTGGACAA GCTGTTCATC CAGCTGGTGC
     2221 AGACCTACAA CCAGCTGTTC GAGGAAAACC CCATCAACGC CAGCGGCGTG GACGCCAAGG
     2281 CCATCCTGTC TGCCAGACTG AGCAAGAGCA GACGGCTGGA AAATCTGATC GCCCAGCTGC
     2341 CCGGCGAGAA GAAGAATGGC CTGTTCGGAA ACCTGATTGC CCTGAGCCTG GGCCTGACCC
     2401 CCAACTTCAA GAGCAACTTC GACCTGGCCG AGGATGCCAA ACTGCAGCTG AGCAAGGACA
     2461 CCTACGACGA CGACCTGGAC AACCTGCTGG CCCAGATCGG CGACCAGTAC GCCGACCTGT
     2521 TTCTGGCCGC CAAGAACCTG TCCGACGCCA TCCTGCTGAG CGACATCCTG AGAGTGAACA
     2581 CCGAGATCAC CAAGGCCCCC CTGAGCGCCT CTATGATCAA GAGATACGAC GAGCACCACC
     2641 AGGACCTGAC CCTGCTGAAA GCTCTCGTGC GGCAGCAGCT GCCTGAGAAG TACAAAGAGA
     2701 TTTTCTTCGA CCAGAGCAAG AACGGCTACG CCGGCTACAT TGACGGCGGA GCCAGCCAGG
     2761 AAGAGTTCTA CAAGTTCATC AAGCCCATCC TGGAAAAGAT GGACGGCACC GAGGAACTGC
     2821 TCGTGAAGCT GAACAGAGAG GACCTGCTGC GGAAGCAGCG GACCTTCGAC AACGGCAGCA
     2881 TCCCCCACCA GATCCACCTG GGAGAGCTGC ACGCCATTCT GCGGCGGCAG GAAGATTTTT
     2941 ACCCATTCCT GAAGGACAAC CGGGAAAAGA TCGAGAAGAT CCTGACCTTC CGCATCCCCT
     3001 ACTACGTGGG CCCTCTGGCC AGGGGAAACA GCAGATTCGC CTGGATGACC AGAAAGAGCG
     3061 AGGAAACCAT CACCCCCTGG AACTTCGAGG AAGTGGTGGA CAAGGGCGCT TCCGCCCAGA
     3121 GCTTCATCGA GCGGATGACC AACTTCGATA AGAACCTGCC CAACGAGAAG GTGCTGCCCA
     3181 AGCACAGCCT GCTGTACGAG TACTTCACCG TGTATAACGA GCTGACCAAA GTGAAATACG
     3241 TGACCGAGGG AATGAGAAAG CCCGCCTTCC TGAGCGGCGA GCAGAAAAAG GCCATCGTGG
     3301 ACCTGCTGTT CAAGACCAAC CGGAAAGTGA CCGTGAAGCA GCTGAAAGAG GACTACTTCA
     3361 AGAAAATCGA GTGCTTCGAC TCCGTGGAAA TCTCCGGCGT GGAAGATCGG TTCAACGCCT
     3421 CCCTGGGCAC ATACCACGAT CTGCTGAAAA TTATCAAGGA CAAGGACTTC CTGGACAATG
     3481 AGGAAAACGA GGACATTCTG GAAGATATCG TGCTGACCCT GACACTGTTT GAGGACAGAG
     3541 AGATGATCGA GGAACGGCTG AAAACCTATG CCCACCTGTT CGACGACAAA GTGATGAAGC
     3601 AGCTGAAGCG GCGGAGATAC ACCGGCTGGG GCAGGCTGAG CCGGAAGCTG ATCAACGGCA
     3661 TCCGGGACAA GCAGTCCGGC AAGACAATCC TGGATTTCCT GAAGTCCGAC GGCTTCGCCA
     3721 ACAGAAACTT CATGCAGCTG ATCCACGACG ACAGCCTGAC CTTTAAAGAG GACATCCAGA
     3781 AAGCCCAGGT GTCCGGCCAG GGCGATAGCC TGCACGAGCA CATTGCCAAT CTGGCCGGCA
     3841 GCCCCGCCAT TAAGAAGGGC ATCCTGCAGA CAGTGAAGGT GGTGGACGAG CTCGTGAAAG
     3901 TGATGGGCCG GCACAAGCCC GAGAACATCG TGATCGAAAT GGCCAGAGAG AACCAGACCA
     3961 CCCAGAAGGG ACAGAAGAAC AGCCGCGAGA GAATGAAGCG GATCGAAGAG GGCATCAAAG
     4021 AGCTGGGCAG CCAGATCCTG AAAGAACACC CCGTGGAAAA CACCCAGCTG CAGAACGAGA
     4081 AGCTGTACCT GTACTACCTG CAGAATGGGC GGGATATGTA CGTGGACCAG GAACTGGACA
     4141 TCAACCGGCT GTCCGACTAC GATGTGGACC ATATCGTGCC TCAGAGCTTT CTGAAGGACG
     4201 ACTCCATCGA CAACAAGGTG CTGACCAGAA GCGACAAGAA CCGGGGCAAG AGCGACAACG
     4261 TGCCCTCCGA AGAGGTCGTG AAGAAGATGA AGAACTACTG GCGGCAGCTG CTGAACGCCA
     4321 AGCTGATTAC CCAGAGAAAG TTCGACAATC TGACCAAGGC CGAGAGAGGC GGCCTGAGCG
     4381 AACTGGATAA GGCCGGCTTC ATCAAGAGAC AGCTGGTGGA AACCCGGCAG ATCACAAAGC
     4441 ACGTGGCACA GATCCTGGAC TCCCGGATGA ACACTAAGTA CGACGAGAAT GACAAGCTGA
     4501 TCCGGGAAGT GAAAGTGATC ACCCTGAAGT CCAAGCTGGT GTCCGATTTC CGGAAGGATT
     4561 TCCAGTTTTA CAAAGTGCGC GAGATCAACA ACTACCACCA CGCCCACGAC GCCTACCTGA
     4621 ACGCCGTCGT GGGAACCGCC CTGATCAAAA AGTACCCTAA GCTGGAAAGC GAGTTCGTGT
     4681 ACGGCGACTA CAAGGTGTAC GACGTGCGGA AGATGATCGC CAAGAGCGAG CAGGAAATCG
     4741 GCAAGGCTAC CGCCAAGTAC TTCTTCTACA GCAACATCAT GAACTTTTTC AAGACCGAGA
     4801 TTACCCTGGC CAACGGCGAG ATCCGGAAGC GGCCTCTGAT CGAGACAAAC GGCGAAACCG
     4861 GGGAGATCGT GTGGGATAAG GGCCGGGATT TTGCCACCGT GCGGAAAGTG CTGAGCATGC
     4921 CCCAAGTGAA TATCGTGAAA AAGACCGAGG TGCAGACAGG CGGCTTCAGC AAAGAGTCTA
     4981 TCCTGCCCAA GAGGAACAGC GATAAGCTGA TCGCCAGAAA GAAGGACTGG GACCCTAAGA
     5041 AGTACGGCGG CTTCGACAGC CCCACCGTGG CCTATTCTGT GCTGGTGGTG GCCAAAGTGG
     5101 AAAAGGGCAA GTCCAAGAAA CTGAAGAGTG TGAAAGAGCT GCTGGGGATC ACCATCATGG
     5161 AAAGAAGCAG CTTCGAGAAG AATCCCATCG ACTTTCTGGA AGCCAAGGGC TACAAAGAAG
     5221 TGAAAAAGGA CCTGATCATC AAGCTGCCTA AGTACTCCCT GTTCGAGCTG GAAAACGGCC
     5281 GGAAGAGAAT GCTGGCCTCT GCCGGCGAAC TGCAGAAGGG AAACGAACTG GCCCTGCCCT
     5341 CCAAATATGT GAACTTCCTG TACCTGGCCA GCCACTATGA GAAGCTGAAG GGCTCCCCCG
     5401 AGGATAATGA GCAGAAACAG CTGTTTGTGG AACAGCACAA GCACTACCTG GACGAGATCA
     5461 TCGAGCAGAT CAGCGAGTTC TCCAAGAGAG TGATCCTGGC CGACGCTAAT CTGGACAAAG
     5521 TGCTGTCCGC CTACAACAAG CACCGGGATA AGCCCATCAG AGAGCAGGCC GAGAATATCA
     5581 TCCACCTGTT TACCCTGACC AATCTGGGAG CCCCTGCCGC CTTCAAGTAC TTTGACACCA
     5641 CCATCGACCG GAAGAGGTAC ACCAGCACCA AAGAGGTGCT GGACGCCACC CTGATCCACC
     5701 AGAGCATCAC CGGCCTGTAC GAGACACGGA TCGACCTGTC TCAGCTGGGA GGCGACAAGC
     5761 GACCTGCCGC CACAAAGAAG GCTGGACAGG CTAAGAAGAA GAAAGATTAC AAAGACGATG
     5821 ACGATAAGGG ATCCGGCGCA ACAAACTTCT CTCTGCTGAA ACAAGCCGGA GATGTCGAAG
     5881 AGAATCCTGG ACCGatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     5941 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     6001 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     6061 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     6121 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     6181 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     6241 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taaacgcgtt
     6301 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6361 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6421 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6481 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6541 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6601 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6661 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     6721 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6781 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6841 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     6901 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6961 ggggggactg gaagggctaa ttcactccca acgaagacaa gatttcacca ttatcgtttc
     7021 agacccacct cccaaccccg aggggaccca gagagggcct atttcccatg attccttcat
     7081 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
     7141 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     7201 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     7261 atttcttggc tttatatatc ttgtggaaag gacgaaacac cggagacggt tgtaaatgag
     7321 cacacaaaat acacatgcta aaatattata ttctatgacc tttataaaat caaccaaaat
     7381 cttcttttta ataactttag tatcaataat tagaattttt atgttccttt ttgcaaactt
     7441 ttaataaaaa tgagcaaaat aaaaaaacgc tagttttagt aactcgcgtt gttttcttca
     7501 cctttaataa tagctactcc accacttgtt cctaagcggt cagctcctgc ttcaatcatt
     7561 ttttgagcat cttcaaatgt tctaactcca ccagctgctt taactaaagc attgtcttta
     7621 acaactgact tcattagttt aacatcttca aatgttgcac ctgattttga aaatcctgtt
     7681 gatgttttaa caaattctaa tccagcttca acagctattt cacaagcttt catgatttct
     7741 tcttttgtta ataaacaatt ttccataata catttaacaa catgtgatcc agctgctttt
     7801 tttacagctt tcatgtcttc taaaactaat tcataatttt tgtcttttaa tgcaccaata
     7861 tttaatacca tatcaatttc tgttgcacca tctttaattg cttcagaaac ttcgaatgct
     7921 tttgtagctg ttgtgcatgc acctagagga aaacctacaa catttgttat tcctacattt
     7981 gtgcctttta ataattcttt acaatagctt gttcaatatg aattaacaca aactgttgca
     8041 aaatcaaatt caattgcttc atcacataat tgtttaattt cagctttcgt agcatcttgt
     8101 tttaataatg tgtgatctat atatttgttt agtttcattt tttctcctat atattcattt
     8161 ttaattttaa ttctttaata atttcgtcta ctttaacttt agcgttttga acagattcac
     8221 caacacctat aaaataaatt tttagtttag gttcagttcc acttgggcga acagcaaatc
     8281 atgacttatc ttctaaataa aattttagta agtcttgtcc tggcatatta tacattccat
     8341 cgatgtagtc ttcaacatta acaactttaa gtccagcaat ttgagttaag ggtgttgctc
     8401 tcaatgattt cattaatggt tcaattttta atttcttttc ttctggttta aaattcaagt
     8461 ttaaagtgaa agtgtaatat gcacccattt ctttaaataa atcttctaaa tagtctacta
     8521 atgttttatt ttgtttttta taaaatcaag cagcctctgc tattaatata gaagcttgta
     8581 ttccatcttt atctctagct gagtcatcaa ttacatatcc ataactttct tcataagcaa
     8641 aaacaaaatt taatccgtta tcttcttctt tagcaatttc tctacccatt catttaaatc
     8701 cagttaaagt ttttacaata ttaactccat atttttcatg agcgattcta tcacccaaat
     8761 cacttgttac aaaacttgaa tatagagccg gattttttgg aatgctattt aagcgtttta
     8821 gatttgataa ttttcaatca attaaaattg gtcctgtttg atttccatct aatcttacaa
     8881 aatgaccatc atgttttatt gccattccaa atctgtcagc atctgggtca ttcataataa
     8941 taatatctgc atcatgttta ataccatatt caagcggtat ttttcatgca ggatcaaatt
     9001 ctggatttgg atttacaaca tttttaaatg tttcatcttc aaatgcatgc tcttcaacct
     9061 caataacgtt atatcctgat tcacgtaata tttttggggt aaatttagtt cctgttccat
     9121 taactgcgct aaaaataatt tttaaatctt ttttagcttc ttgctctttt ttgtacgtct
     9181 ctgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
     9241 gtggcaccga gtcggtgctt ttttaagctt ggcgtaacta gatcttgaga cactgctttt
     9301 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     9361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     9421 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     9481 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     9541 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     9601 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9661 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     9721 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9781 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9841 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     9901 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     9961 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
    10021 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
    10081 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
    10141 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
    10201 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
    10261 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
    10321 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
    10381 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
    10441 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
    10501 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
    10561 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
    10621 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
    10681 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
    10741 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
    10801 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
    10861 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
    10921 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
    10981 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
    11041 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
    11101 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
    11161 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
    11221 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    11281 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    11341 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    11401 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    11461 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    11521 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    11581 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    11641 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    11701 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    11761 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    11821 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    11881 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    11941 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    12001 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    12061 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    12121 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    12181 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    12241 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    12301 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    12361 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
    12421 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
    12481 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
    12541 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    12601 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    12661 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
    12721 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
    12781 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
    12841 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
    12901 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
    12961 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    13021 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    13081 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    13141 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
    13201 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
    13261 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
    13321 gcgtcagtat taagcggggg agaattagat
//