LOCUS [GibAss]_pSV40-mCherry- 5556 bp ds-DNA circular 09-JAN-2018 DEFINITION . FEATURES Location/Qualifiers primer complement(4091..4110) /label="EBV_rev_primer" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e terminator 1647..1695 /label="synth_PA_terminator" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e origin 1078..1384 /label="f1_origin" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 gene complement(69..929) /label="Ampicillin" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 promoter 1855..2057 /label="SV40_promoter" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 terminator 1647..1800 /label="up_PA_terminator" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 regulatory 1647..1800 /label="TB" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer 4523..4543 /label="pBABE_3_primer" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 2795..2860 /label="P2A" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 2081..2086 /label="kozak" /ApEinfo_revcolor=#7ea7d8 /ApEinfo_fwdcolor=#7ea7d8 terminator complement(4077..4196) /label="SV40_PA_terminator" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer 1964..1983 /label="SV40pro_F_primer" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 2087..2794 /label="mCherry" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 2861..3886 /label="Hygromycin R" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer 1749..1768 /label="RVprimer3_primer" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc promoter complement(971..999) /label="AmpR_promoter" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 origin 1902..1979 /label="SV40_origin" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 regulatory 4316..4543 /label="SV40_enhancer" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 ORIGIN 1 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 61 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 121 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 181 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 241 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 301 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 361 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 421 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 481 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 541 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 601 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 661 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 721 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 781 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 841 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 901 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 961 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 1021 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca 1081 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 1141 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 1201 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 1261 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 1321 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 1381 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 1441 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata 1501 ttaacgctta caatttgcca ttcgccattc aggctgcgca actgttggga agggcgatcg 1561 gtgcgggcct cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag 1621 gtacgggagg tacttggagc ggccgcaata aaatatcttt attttcatta catctgtgtg 1681 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac 1741 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct 1801 atcgataggt accgagctct tacgcgtgct agcccgggct cgagatctgc gatctgcatc 1861 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 1921 ccagttccgc ccattctccg ccccatcgct gactaatttt ttttatttat gcagaggccg 1981 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 2041 gcttttgcaa aaagcttggc attccggtac tgttggtaaa gccaccatgg tgagcaaggg 2101 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 2161 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 2221 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 2281 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 2341 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 2401 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta 2461 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 2521 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 2581 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 2641 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 2701 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 2761 ccactccacc ggcggcatgg acgagctgta caagGGATCC GGCGCAACAA ACTTCTCTCT 2821 GCTGAAACAA GCCGGAGATG TCGAAGAGAA TCCTGGACCG atgaaaaagc ctgaactcac 2881 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca 2941 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt 3001 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt 3061 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattta gcgagagcct 3121 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga 3181 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct 3241 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg 3301 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga 3361 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga 3421 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga 3481 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata 3541 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg 3601 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct 3661 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc 3721 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac 3781 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga 3841 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagttct agacaaacac 3901 catacaacaa gaaacaaaca ccatacaaca agaaacaaac accatacaac aagaaacaaa 3961 caccatacaa caagaaacaa acaccataca acaagaaaca aacaccatac aacaagaaac 4021 aaacaccata caacaagaaa gggcccgtcg gggcggccgg ccgcttcgag cagacatgat 4081 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat 4141 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt 4201 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt 4261 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatctga acgatggagc 4321 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact 4381 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg 4441 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct 4501 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagcgg atccgtcgac 4561 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta 4621 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag 4681 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 4741 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 4801 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 4861 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 4921 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 4981 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 5041 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 5101 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 5161 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 5221 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 5281 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 5341 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 5401 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 5461 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 5521 ttggtcatga gattatcaaa aaggatcttc acctag //