LOCUS       [GibAss]_pSV40-mCherry- 5556 bp ds-DNA     circular     09-JAN-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          complement(4091..4110)
                     /label="EBV_rev_primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      1647..1695
                     /label="synth_PA_terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     origin          1078..1384
                     /label="f1_origin"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     gene            complement(69..929)
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        1855..2057
                     /label="SV40_promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      1647..1800
                     /label="up_PA_terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     regulatory      1647..1800
                     /label="TB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          4523..4543
                     /label="pBABE_3_primer"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2795..2860
                     /label="P2A"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2081..2086
                     /label="kozak"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     terminator      complement(4077..4196)
                     /label="SV40_PA_terminator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1964..1983
                     /label="SV40pro_F_primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2087..2794
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2861..3886
                     /label="Hygromycin R"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          1749..1768
                     /label="RVprimer3_primer"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(971..999)
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     origin          1902..1979
                     /label="SV40_origin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     regulatory      4316..4543
                     /label="SV40_enhancer"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
       61 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
      121 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
      181 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
      241 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
      301 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
      361 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
      421 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
      481 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
      541 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
      601 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
      661 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
      721 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
      781 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
      841 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
      901 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
      961 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     1021 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
     1081 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     1141 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     1201 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     1261 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     1321 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     1381 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     1441 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     1501 ttaacgctta caatttgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     1561 gtgcgggcct cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag
     1621 gtacgggagg tacttggagc ggccgcaata aaatatcttt attttcatta catctgtgtg
     1681 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac
     1741 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
     1801 atcgataggt accgagctct tacgcgtgct agcccgggct cgagatctgc gatctgcatc
     1861 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     1921 ccagttccgc ccattctccg ccccatcgct gactaatttt ttttatttat gcagaggccg
     1981 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     2041 gcttttgcaa aaagcttggc attccggtac tgttggtaaa gccaccatgg tgagcaaggg
     2101 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     2161 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     2221 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     2281 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     2341 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     2401 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     2461 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     2521 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     2581 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     2641 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     2701 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     2761 ccactccacc ggcggcatgg acgagctgta caagGGATCC GGCGCAACAA ACTTCTCTCT
     2821 GCTGAAACAA GCCGGAGATG TCGAAGAGAA TCCTGGACCG atgaaaaagc ctgaactcac
     2881 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     2941 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     3001 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     3061 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattta gcgagagcct
     3121 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     3181 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     3241 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     3301 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     3361 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     3421 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     3481 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     3541 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     3601 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     3661 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     3721 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     3781 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     3841 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagttct agacaaacac
     3901 catacaacaa gaaacaaaca ccatacaaca agaaacaaac accatacaac aagaaacaaa
     3961 caccatacaa caagaaacaa acaccataca acaagaaaca aacaccatac aacaagaaac
     4021 aaacaccata caacaagaaa gggcccgtcg gggcggccgg ccgcttcgag cagacatgat
     4081 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     4141 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4201 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     4261 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatctga acgatggagc
     4321 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
     4381 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     4441 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     4501 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagcgg atccgtcgac
     4561 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     4621 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     4681 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4741 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4801 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4861 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4921 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4981 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5041 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5101 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5161 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     5221 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5281 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     5341 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     5401 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     5461 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     5521 ttggtcatga gattatcaaa aaggatcttc acctag
//