LOCUS       pHW522_Prab_3_cG        4492 bp ds-DNA     circular     21-JAN-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     pHW522	Prab-3::NLS::gp41-1-C-intein::cGAL(AD)::let858 3UTR 
COMMENT     the 5 end and 3 end of Prab-3 and the entire C-intein-VP64
            confirmed by Han with oHW10f and oHW233r, 20170330
COMMENT     insert:  Prab-3 (see pHW393) FseI/AscI
COMMENT     vector: pAH36 (FseI/AscI)
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     4347..4367
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     1349..1372
                     /label=NLS
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=#66ffcc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1550..1582
                     /label=VP16 domain
                     /ApEinfo_fwdcolor=#00ff80
                     /ApEinfo_revcolor=#00ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1589..1621
                     /label=VP16 domain(1)
                     /ApEinfo_label=VP16 domain
                     /ApEinfo_fwdcolor=#00ff80
                     /ApEinfo_revcolor=#00ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      3297..3979
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1628..1660
                     /label=VP16 domain(2)
                     /ApEinfo_label=VP16 domain
                     /ApEinfo_fwdcolor=#00ff80
                     /ApEinfo_revcolor=#00ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1667..1699
                     /label=VP16 domain(3)
                     /ApEinfo_label=VP16 domain
                     /ApEinfo_fwdcolor=#00ff80
                     /ApEinfo_revcolor=#00ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    4319..4341
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             2540..3199
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1379..1492
                     /label=gp41 C-intein C elegans codon optimzed
                     /ApEinfo_fwdcolor=#408080
                     /ApEinfo_revcolor=#00ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           1745..2177
                     /label=let-858 3UTR
                     /ApEinfo_fwdcolor=#bfddbb
                     /ApEinfo_revcolor=#bfddbb
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1493..1504
                     /label=SSDV for intein splicing
                     /ApEinfo_fwdcolor=#800080
                     /ApEinfo_revcolor=#00ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    37..1244
                     /label=1.2kb Rab-3b promoter for Pan-neuronal expression
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        join(4432..4492,1..2)
                     /label=decoy ORF
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          4382..4431
                     /label=decoy intron
                     /ApEinfo_fwdcolor=#b3b3b3
                     /ApEinfo_revcolor=#b3b3b3
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 AAGCTTgcat gcgcggccgc actgactgGG CCGGCCgatc ttcagatggg agcagtggac
       61 tgtctatttg atattcttaa aatgtactct ataaaagatg aaactgtaat gaccaattcc
      121 ctacataaat atttttaact aattggtcgg atatttgggg atcagagaag tacttgtttt
      181 tgcgatatta ataatgatat atttttctag taaaaaaatg ttaagcgatt tgtgaataat
      241 taagttggct tgaaaaagat tttttgatag ctatgtattt tgttaattta aatcagattt
      301 taaaacttta ttattgacta tttatatata ttatatatta tatattttca tggtttaatc
      361 aaaatctatt aaattatttc taaaatctct aaatatcgaa ttctctaaaa agcttcatca
      421 acaaaagcat ttcaattatc taacacaatg agaataattc aagaactgaa atagaaagaa
      481 atctagaaaa aaaagagagt gaggtgccct attaaatcgg tgctctcctc tctaacagaa
      541 gcgcctacac gcacccaaga gagctctttt tctttctttt ttcggagcac acatacacgc
      601 atacactcct cagaagtgta tagagaaaat gggagaagaa gcgaaagaag acgaagaagc
      661 ggttgtgatg tatgcatcgg gcgtttctat ctctctccgt gagcaacgag ctagtcaacc
      721 catcgatcca tttttgtgag cacacacaga gagagactca aacacactca cagcttcaca
      781 ctgagctcca agagctctgt agagagaagc cagagtatgc tttcctcgat tttcctaacg
      841 tcttcttctt ctgctttttt ctttttccca acgagttgta gtccactaac ttcaattttt
      901 cctccagaga aagagtttta gagagacagc tgataaatac actagagaaa taaaaagaat
      961 gataaaaatt tgctatttta atcagttccc tctcgtttct ctaagttttg ttgttgggtt
     1021 gggaaattaa aattggaaaa tggaaataac gtttttttct gtctaatctt gaattctcca
     1081 gacatttctt atttcctttt ttgttgcaag tttttcttcc ttctctaatt tttaatcaaa
     1141 gatatctcgc tatgaacttt gaatttttga tattttgatt ctataaatac ataaagtaat
     1201 ctaaatgctc ttttaaaata aatctacagt agccctattt tcagGGCGCG CCtctagagg
     1261 atccccggga ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag
     1321 aacattttca ggaggaccct tgGCTAGGat gggccctaaa aagaagcgta aaGCTAGCAT
     1381 GATGTTGAAG AAGATTCTCA AAATTGAAGA ACTGGATGAG CGTGAGCTCA TCGACATCGA
     1441 GGTCTCCGGA AACCACCTCT TCTACGCCAA CGACATCCTC ACCCACAACT CCTCCTCCGA
     1501 TGTACTGCAG tctggtggcg gaggggctCC TAGGGGTTCC GGACGGGCTG ACGCATTGGA
     1561 CGATTTTGAT CTGGATATGC TGGGAAGTGA CGCCCTCGAT GATTTTGACC TTGACATGCT
     1621 TGGTTCGGAT GCCCTTGATG ACTTTGACCT CGACATGCTC GGCAGTGACG CCCTTGATGA
     1681 TTTCGACCTG GACATGCTGA TTAACTAAGG TACCGAATTC GCTAGCCGGC CATACAAGTA
     1741 ATCCGGATGA TCGACGCCaA CGTCGTTGAA TTTTCAAATT TTAAATACTG AATATTTGTT
     1801 TTTTTTCCTA TTATTTATTT ATTCTCTTTG TGTTTTTTTT CTTGCTTTCT AAAAAATTAA
     1861 TTCAATCCAA ATCTAAacat ttttttttct ctttccgtct cccaattcgt attccgctcc
     1921 tctcatctga acacaatgtg caagtttatt tatcttctcg ctttcatttc attaggacgt
     1981 ggggggaatt ggtggaaggg ggaaacacac aaaaggatga tggaaatgaa ataaggacac
     2041 acaatatgca acaacattca attcagaaat atggaggaag gtttaaaaga aaacataaaa
     2101 atatatagag gaggaaggaa aactagtaaa aaataagcaa agaaattagg cgaacgatgA
     2161 GAATTGTCCT CGCTTGGGCC CAAAAGGCCT TTTTTTTTTG GCGCCGACGT CAGGTGGCAC
     2221 TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT
     2281 GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG
     2341 TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC
     2401 TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC
     2461 ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC
     2521 CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC
     2581 CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT
     2641 GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT
     2701 ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT
     2761 CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT
     2821 TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT
     2881 GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC
     2941 TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG
     3001 CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC
     3061 TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA
     3121 CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC
     3181 CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA
     3241 TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT
     3301 GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT
     3361 CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA
     3421 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA
     3481 GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT
     3541 AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT
     3601 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA
     3661 GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT
     3721 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC
     3781 GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA
     3841 GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG
     3901 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA
     3961 AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT
     4021 GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC
     4081 TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA
     4141 AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG
     4201 GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA
     4261 GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG
     4321 AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT
     4381 gtaagtttaa acatgatctt actaactaac tattctcatt taaattttca gAGCTTAAAA
     4441 ATGGCTGAAA TCACTCACAA CGATGGATAC GCTAACAACT TGGAAATGAA AT
//
