LOCUS       pHW533_Peft_3_cG        4313 bp ds-DNA     circular     21-JAN-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     pHW533	Peft-3::NLS::cGAL(DBD)::gp41-1-N-intein::let-858 3'UTR
COMMENT     the 5 end of Peft-3 and the 3 end of the cGAL-gp41-1-N-intein
            confirmed by Han with oHW10f and oHW233r, 20170403
COMMENT     insert:  ~700bp Peft-3 PCR with oHW406f and oHW112r from pHW341
COMMENT     vector:: pAH35(FseI/AscI)
COMMENT     FseI/AscI Ligation
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     4168..4188
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     750..773
                     /label=NLS
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=#66ffcc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      3118..3800
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    4140..4162
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        37..645
                     /label=eft-3 promoter
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1263..1526
                     /label=gp41 N-intein C elegans codon optimzed
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#00ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             2361..3020
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           1566..1998
                     /label=let-858 3UTR
                     /ApEinfo_fwdcolor=#bfddbb
                     /ApEinfo_revcolor=#bfddbb
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        join(4253..4313,1..2)
                     /label=decoy ORF
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          4203..4252
                     /label=decoy intron
                     /ApEinfo_fwdcolor=#b3b3b3
                     /ApEinfo_revcolor=#b3b3b3
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             780..1220
                     /label=SK GAL4
                     /ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_revcolor=#00ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 AAGCTTgcat gcgcggccgc actgactgGG CCGGCCGCAC CTTTGGTCTT TTATTGTCAA
       61 CTTCCATTGG TTCTTCCATT GTTTCTGTTA AATTAATGAA TTTTTCATAA AATAAAGACA
      121 TTATACAATA TAAAAATGAA GAATTTATTG AAAATAAACT GCCAGAGAGA AAAAGTATGC
      181 AACACTCCCG CCGAGAGTGT TTGAAATGGT GTACGGTACA TTTTCGTGCT AGGAGTTAGA
      241 TGTGCAGGCA GCAACGAGAG GGGGAGAGAT TTTTTTGGGC CTTGTGAAAT TAACGTGAGT
      301 TTTCTGGTCA TCTGACTAAT CATGTTGGTT TTTTGTTGGT TTATTTTGTT TTTATCTTTG
      361 TTTTTATCCA GATTAGGAAA TTTAAATTTT ATGAATTTAT AATGAGGTCA AACATTCAGT
      421 CCCAGCGTTT TTCCTGTTCT CACTGTTTAG TCGAATTTTT ATTTTAGGCT TTCAACAAAT
      481 GTTCTAACTG TCTTATTTGT GACCTCACTT TTTATATTTT TTTAATTTTT AAAAATATTA
      541 GAAGTTTCTA GGATAATTTT TTCGACTTTT ATTCTCTCTA CCGTCCGCAC TCTTCTTACT
      601 TTTAAATTAA ATTGTTTTTT TTTCAGTTGG GAAACACTTT GCTCAGGCGC GCCtctagag
      661 gatccccggg attggccaaa ggacccaaag gtatgtttcg aatgatacta acataacata
      721 gaacattttc aggaggaccc ttgGCTAGGa tgggccctaa aaagaagcgt aaaGCTAGCA
      781 TGAAGCTGTT GTCTTCAATG GAACAAGCTT GCGACATATG CCGGTTAAAA AAACTCAGGT
      841 GCTCGAAGGA AAAGCCCAAG TGTGCCAAGT GTCTGAAGAA CAACTCGGAG TGCCGTTATT
      901 CTCCCAAGGC TATGAGGTCC CCACTGACCA GGGCGCATCT GACAGAGGTA GAATCAAGGT
      961 TGGAAAAATT GGAAGATTTG TTTCTGCTCA TGTTTCCTCG GGAAAACCTG GACAGCATTT
     1021 TGAACATGGA CTCTTTGGAC GAAGTGAAAG TAATGTTGAA ACAATTATAT ATGCAGGACT
     1081 ATGGTGACAC GGATGCTGCT ATGGATAGCT TGACCCCTAT GGAAAATGGC ATGTCTGAAG
     1141 GGCCGAGAAA ACACATGAAG ACTGCGACAT CATCGCCAAA AGTGGATGAA GATGGAAGCC
     1201 AAAGTCAGTT GAGTGTATCG ccggaattcc cgCTGCAGtc tggtggcgga ggggctCCTA
     1261 GGTGCCTCGA CCTCAAGACC CAAGTCCAAA CCCCACAAGG AATGAAGGAG ATCTCCAACA
     1321 TCCAAGTCGG AGACCTCGTC CTCTCCAACA CCGGATACAA CGAGGTCCTC AACGTCTTCC
     1381 CAAAGTCCAA GAAGAAGTCC TACAAGATCA CCCTCGAGGA CGGAAAGGAG ATCATCTGCT
     1441 CCGAGGAGCA CCTCTTCCCA ACCCAAACCG GAGAGATGAA CATCTCCGGA GGACTCAAGG
     1501 AGGGAATGTG CCTCTACGTC AAGGAGTAAG GTACCGAATT CGCTAGCCGG CCATACAAGT
     1561 AATCCGGATG ATCGACGCCa ACGTCGTTGA ATTTTCAAAT TTTAAATACT GAATATTTGT
     1621 TTTTTTTCCT ATTATTTATT TATTCTCTTT GTGTTTTTTT TCTTGCTTTC TAAAAAATTA
     1681 ATTCAATCCA AATCTAAaca tttttttttc tctttccgtc tcccaattcg tattccgctc
     1741 ctctcatctg aacacaatgt gcaagtttat ttatcttctc gctttcattt cattaggacg
     1801 tggggggaat tggtggaagg gggaaacaca caaaaggatg atggaaatga aataaggaca
     1861 cacaatatgc aacaacattc aattcagaaa tatggaggaa ggtttaaaag aaaacataaa
     1921 aatatataga ggaggaagga aaactagtaa aaaataagca aagaaattag gcgaacgatg
     1981 AGAATTGTCC TCGCTTGGGC CCAAAAGGCC TTTTTTTTTT GGCGCCGACG TCAGGTGGCA
     2041 CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA
     2101 TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA
     2161 GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC
     2221 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG
     2281 CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC
     2341 CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT
     2401 CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT
     2461 TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT
     2521 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA
     2581 TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC
     2641 TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA
     2701 TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG
     2761 CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC
     2821 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT
     2881 CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT
     2941 ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG
     3001 CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG
     3061 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA
     3121 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA
     3181 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA
     3241 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA
     3301 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT
     3361 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT
     3421 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT
     3481 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT
     3541 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCATTGA GAAAGCGCCA
     3601 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG
     3661 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC
     3721 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA
     3781 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA
     3841 TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG
     3901 CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG
     3961 AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT
     4021 GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT
     4081 AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG
     4141 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC
     4201 Tgtaagttta aacatgatct tactaactaa ctattctcat ttaaattttc agAGCTTAAA
     4261 AATGGCTGAA ATCACTCACA ACGATGGATA CGCTAACAAC TTGGAAATGA AAT
//
