LOCUS       pAH34_Phsp16_cGA        4086 bp ds-DNA     circular     21-JAN-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     pAH34	Phsp-16.41::NLS::cGAL(DBD)::gp41-1-N-intein::let-858 3'UTR
COMMENT     FseI-Phsp-16.41 fwd: gaaGGCCGGCCACGTTGAGCTGGACGGAAATAG
COMMENT     AscI-Phsp-16.41 rev: gtgGGCGCGCCTTTCGAAGTTTTTTAGATGCACTAG
COMMENT     All three clones are correct
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     3941..3961
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     523..546
                     /label=NLS
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=#66ffcc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      2891..3573
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        37..418
                     /label=Phsp-16.41
                     /ApEinfo_fwdcolor=#ccff66
                     /ApEinfo_revcolor=#ccff66
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    3913..3935
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1036..1299
                     /label=gp41 N-intein C elegans codon optimzed
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#00ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             2134..2793
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           1339..1771
                     /label=let-858 3UTR
                     /ApEinfo_fwdcolor=#bfddbb
                     /ApEinfo_revcolor=#bfddbb
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        join(4026..4086,1..2)
                     /label=decoy ORF
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          3976..4025
                     /label=decoy intron
                     /ApEinfo_fwdcolor=#b3b3b3
                     /ApEinfo_revcolor=#b3b3b3
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             553..993
                     /label=SK GAL4
                     /ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_revcolor=#00ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 AAGCTTgcat gcgcggccgc actgactgGG CCGGCCACGT TGAGCTGGAC GGAAATAGTG
       61 GTAAAGTGAC ATGATTATAG TTTGAAGATT TCTAATTTCA CAATTAGAGC AAATGTTGTT
      121 CGGTATTTAT TTTCAACGGT ATTTATACTA TTTTCCACCT TTTTCTAGAA CATTCGAGCT
      181 GCTTGTTGCA AAAGGAGGGC GACTCACATT CGGTACATGG AAAAGTAGTG TACACAATAA
      241 AGAGACCCAG ATACATTTTC CGTCTGCGTC TCTTTGCACC CACCGGGAGT ATTTTCAAAC
      301 GAATGCATCT AGGACCTTCT AGAACATTCT GTAAGGCTGC AGAATGCGGG TATATAAGGA
      361 AAGCGGGCTC AGAGGAAGCC AACACGCTTT GTTCTAGTGC ATCTAAAAAA CTTCGAAAGG
      421 CGCGCCtcta gaggatcccc gggattggcc aaaggaccca aaggtatgtt tcgaatgata
      481 ctaacataac atagaacatt ttcaggagga cccttgGCTA GGatgggccc taaaaagaag
      541 cgtaaaGCTA GCATGAAGCT GTTGTCTTCA ATGGAACAAG CTTGCGACAT ATGCCGGTTA
      601 AAAAAACTCA GGTGCTCGAA GGAAAAGCCC AAGTGTGCCA AGTGTCTGAA GAACAACTCG
      661 GAGTGCCGTT ATTCTCCCAA GGCTATGAGG TCCCCACTGA CCAGGGCGCA TCTGACAGAG
      721 GTAGAATCAA GGTTGGAAAA ATTGGAAGAT TTGTTTCTGC TCATGTTTCC TCGGGAAAAC
      781 CTGGACAGCA TTTTGAACAT GGACTCTTTG GACGAAGTGA AAGTAATGTT GAAACAATTA
      841 TATATGCAGG ACTATGGTGA CACGGATGCT GCTATGGATA GCTTGACCCC TATGGAAAAT
      901 GGCATGTCTG AAGGGCCGAG AAAACACATG AAGACTGCGA CATCATCGCC AAAAGTGGAT
      961 GAAGATGGAA GCCAAAGTCA GTTGAGTGTA TCGccggaat tcccgCTGCA Gtctggtggc
     1021 ggaggggctC CTAGGTGCCT CGACCTCAAG ACCCAAGTCC AAACCCCACA AGGAATGAAG
     1081 GAGATCTCCA ACATCCAAGT CGGAGACCTC GTCCTCTCCA ACACCGGATA CAACGAGGTC
     1141 CTCAACGTCT TCCCAAAGTC CAAGAAGAAG TCCTACAAGA TCACCCTCGA GGACGGAAAG
     1201 GAGATCATCT GCTCCGAGGA GCACCTCTTC CCAACCCAAA CCGGAGAGAT GAACATCTCC
     1261 GGAGGACTCA AGGAGGGAAT GTGCCTCTAC GTCAAGGAGT AAGGTACCGA ATTCGCTAGC
     1321 CGGCCATACA AGTAATCCGG ATGATCGACG CCaACGTCGT TGAATTTTCA AATTTTAAAT
     1381 ACTGAATATT TGTTTTTTTT CCTATTATTT ATTTATTCTC TTTGTGTTTT TTTTCTTGCT
     1441 TTCTAAAAAA TTAATTCAAT CCAAATCTAA acattttttt ttctctttcc gtctcccaat
     1501 tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca
     1561 tttcattagg acgtgggggg aattggtgga agggggaaac acacaaaagg atgatggaaa
     1621 tgaaataagg acacacaata tgcaacaaca ttcaattcag aaatatggag gaaggtttaa
     1681 aagaaaacat aaaaatatat agaggaggaa ggaaaactag taaaaaataa gcaaagaaat
     1741 taggcgaacg atgAGAATTG TCCTCGCTTG GGCCCAAAAG GCCTTTTTTT TTTGGCGCCG
     1801 ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA
     1861 ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT
     1921 TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG
     1981 GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA
     2041 GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT
     2101 GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT
     2161 GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT
     2221 TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG
     2281 ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA
     2341 CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT
     2401 CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG
     2461 CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA
     2521 CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA
     2581 GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC
     2641 GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT
     2701 ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC
     2761 GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT
     2821 ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT
     2881 TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC
     2941 CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC
     3001 TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA
     3061 ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA
     3121 GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT
     3181 CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG
     3241 GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC
     3301 ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT
     3361 TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG
     3421 GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT
     3481 CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG
     3541 CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG
     3601 CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC
     3661 GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG
     3721 AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT
     3781 CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA
     3841 ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT
     3901 CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT
     3961 GATTACGCCA AGCTgtaagt ttaaacatga tcttactaac taactattct catttaaatt
     4021 ttcagAGCTT AAAAATGGCT GAAATCACTC ACAACGATGG ATACGCTAAC AACTTGGAAA
     4081 TGAAAT     
//
