LOCUS pAH36_Pmyo_2_cGA 4246 bp ds-DNA circular 21-JAN-2018 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT pAH36 Pmyo-2::NLS::gp41-1-C-intein::cGAL(AD)::let-858 3'UTR COMMENT sequence made by Han COMMENT confirmed by sequencing with oHW10f and oHW233r, Laragen# 125489, at 20160928 COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind 4101..4121 /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_signal 1103..1126 /label=NLS /ApEinfo_fwdcolor=#ff80ff /ApEinfo_revcolor=#66ffcc /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1304..1336 /label=VP16 domain /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1343..1375 /label=VP16 domain(1) /ApEinfo_label=VP16 domain /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 3051..3733 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 37..64 /label=p.myo2F2 primer /ApEinfo_fwdcolor=#ff8040 /ApEinfo_revcolor=#ff8040 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1382..1414 /label=VP16 domain(2) /ApEinfo_label=VP16 domain /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 976..998 /label=p.myo2R2 primer /ApEinfo_fwdcolor=#004080 /ApEinfo_revcolor=#004080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1421..1453 /label=VP16 domain(3) /ApEinfo_label=VP16 domain /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 37..998 /label=pMyo-2 /ApEinfo_fwdcolor=#8000ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding 4073..4095 /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 2294..2953 /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1133..1246 /label=gp41 C-intein C elegans codon optimzed /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#00ff80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 3'UTR 1499..1931 /label=let-858 3UTR /ApEinfo_fwdcolor=#bfddbb /ApEinfo_revcolor=#bfddbb /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1247..1258 /label=SSDV for intein splicing /ApEinfo_fwdcolor=#800080 /ApEinfo_revcolor=#00ff80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter join(4186..4246,1..2) /label=decoy ORF /ApEinfo_fwdcolor=#999999 /ApEinfo_revcolor=#999999 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 intron 4136..4185 /label=decoy intron /ApEinfo_fwdcolor=#b3b3b3 /ApEinfo_revcolor=#b3b3b3 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 AAGCTTgcat gcgcggccgc actgactgGG CCGGCCgtag tatcctttgc tttaaatgtc 61 cataaaacta attttataat caataaaaca acgtttgtaa atcaactgag tttacaagta 121 gagacattga gggatacttt cactatgcta aagtgataat cgaccaaata ataacttcac 181 tttggtattt attcctgtct tataaatgtt atgtatgaat taaattcata tgcatatggc 241 tcactctgac aatttttaat aatcttccag atcaatattg actaccgatg cgggtggtct 301 tttgctttga attctgctga actttacacc ccgaacagca atgtgtgctt cagcctaaaa 361 aaaagtaagt gtgttaatca gtgcccccga ttcttcattt tttgcccctc tctcccgttt 421 cgtcggcaaa agaagagaaa ataaagataa gtctcaagat aggttggtaa tcgctaaagt 481 ggttgtgtgg ataagagtag caaaatggca ggaagagcac tttgcgcgca cacactgtac 541 tcattgttct ggataaaatt ctctcgttgt ttgccgtcgg atgtctgcct ctctgcattg 601 agccggcttc ttcactatct ttagttaacc taaaatgccg tttcttttct cgtatcccca 661 ctatcccgtt gaggttctct gctctcttcg ctccctaccg ccagcgagca actatccgtg 721 ggggcgcctt gctcggaaga tgggggggaa gaaagaagat ttttgctatt tgcacttgag 781 aaagagactt ttcctgcgtc gatggttaga gaacagtgtg cagacacttt tcagctacct 841 agaattacaa ttggatatcc ccgcctccca atccacccac ccagggaaaa agaagggctc 901 gccgaaaatc aaagttatct ccaggctcgc gcatcccacc gagcggttga cttctctcca 961 ccacttttca ttttaaccct cgatcgtcag acacagaaGG CGCGCCtcta gaggatcccc 1021 gggattggcc aaaggaccca aaggtatgtt tcgaatgata ctaacataac atagaacatt 1081 ttcaggagga cccttgGCTA GGatgggccc taaaaagaag cgtaaaGCTA GCATGATGTT 1141 GAAGAAGATT CTCAAAATTG AAGAACTGGA TGAGCGTGAG CTCATCGACA TCGAGGTCTC 1201 CGGAAACCAC CTCTTCTACG CCAACGACAT CCTCACCCAC AACTCCTCCT CCGATGTACT 1261 GCAGtctggt ggcggagggg ctCCTAGGGG TTCCGGACGG GCTGACGCAT TGGACGATTT 1321 TGATCTGGAT ATGCTGGGAA GTGACGCCCT CGATGATTTT GACCTTGACA TGCTTGGTTC 1381 GGATGCCCTT GATGACTTTG ACCTCGACAT GCTCGGCAGT GACGCCCTTG ATGATTTCGA 1441 CCTGGACATG CTGATTAACT AAGGTACCGA ATTCGCTAGC CGGCCATACA AGTAATCCGG 1501 ATGATCGACG CCaACGTCGT TGAATTTTCA AATTTTAAAT ACTGAATATT TGTTTTTTTT 1561 CCTATTATTT ATTTATTCTC TTTGTGTTTT TTTTCTTGCT TTCTAAAAAA TTAATTCAAT 1621 CCAAATCTAA acattttttt ttctctttcc gtctcccaat tcgtattccg ctcctctcat 1681 ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca tttcattagg acgtgggggg 1741 aattggtgga agggggaaac acacaaaagg atgatggaaa tgaaataagg acacacaata 1801 tgcaacaaca ttcaattcag aaatatggag gaaggtttaa aagaaaacat aaaaatatat 1861 agaggaggaa ggaaaactag taaaaaataa gcaaagaaat taggcgaacg atgAGAATTG 1921 TCCTCGCTTG GGCCCAAAAG GCCTTTTTTT TTTGGCGCCG ACGTCAGGTG GCACTTTTCG 1981 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 2041 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG 2101 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT 2161 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT 2221 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA 2281 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT 2341 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA 2401 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG 2461 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG 2521 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG 2581 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT 2641 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG 2701 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC 2761 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG 2821 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC 2881 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 2941 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA 3001 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA 3061 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 3121 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 3181 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 3241 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA 3301 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 3361 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 3421 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 3481 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC 3541 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 3601 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 3661 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 3721 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT 3781 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC 3841 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG 3901 CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA 3961 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC 4021 TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT 4081 GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTgtaagt 4141 ttaaacatga tcttactaac taactattct catttaaatt ttcagAGCTT AAAAATGGCT 4201 GAAATCACTC ACAACGATGG ATACGCTAAC AACTTGGAAA TGAAAT //