LOCUS       Exported                6432 bp ds-DNA     circular SYN 24-JAN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2017 from SnapGene 4.0.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    769..789
                     /label=CMV_F
     CDS             863..879
                     /label=T7 promoter primer
     misc_feature    895..902
                     /label=NotI(1)
     misc_feature    895..896
                     /label=NotI
     misc_feature    910..951
                     /label=V5 tag
     misc_feature    952..957
                     /label=NheI
     CDS             958..1914
                     /codon_start=1
                     /label=BirA (humanized)
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    1147..1149
                     /label=Q65P
     misc_feature    1213..1215
                     /label=I87V
     misc_feature    1297..1299
                     /label=G115G
     misc_feature    1306..1308
                     /label=R118S
     misc_feature    1372..1374
                     /label=E141K
     misc_difference 1375..1377
                     /label=Q142R
     misc_feature    1399..1401
                     /label=S150G
     misc_feature    1402..1404
                     /label=L151P
     misc_feature    1429..1431
                     /label=V160A
     misc_feature    1525..1527
                     /label=T192A
     misc_difference 1531..1533
                     /label=K194I
     misc_feature    1576..1578
                     /label=M209V
     misc_feature    1672..1674
                     /label=M241T
     misc_feature    1738..1740
                     /label=S263P
     misc_structure  1822..1824
                     /label=G291G
     misc_feature    1864..1866
                     /label=I305V
     misc_feature    join(1915..1925,1927..1950)
                     /label=NES
     misc_feature    1951..1953
                     /label=Stop
     misc_feature    1954..1956
                     /label=STOP
     misc_feature    1957..1962
                     /label=XhoI site
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gccaccatgg gcaagcccat ccccaacccc ctgctgggcc tggacagcac cgctagcaaa
      961 gacaatactg tgcctctgaa gctgatcgct ctcctggcta atggcgagtt ccatagtggc
     1021 gaacagctgg gagaaaccct gggcatgtcc agggccgcta tcaacaagca cattcagact
     1081 ctgcgcgact ggggcgtgga cgtgttcacc gtgcccggaa agggctactc tctgcccgag
     1141 cctatcccgc tgctgaacgc taaacagatt ctgggacagc tggacggcgg gagcgtggca
     1201 gtcctgcctg tggtcgactc caccaatcag tacctgctgg atcgaatcgg cgagctgaag
     1261 agtggggatg cttgcattgc agaatatcag caggcaggga gaggaagcag agggaggaaa
     1321 tggttctctc cttttggagc taacctgtac ctgagtatgt tttggcgcct gaagcgggga
     1381 ccagcagcaa tcggcctggg cccggtcatc ggaattgtca tggcagaagc gctgcgaaag
     1441 ctgggagcag acaaggtgcg agtcaaatgg cccaatgacc tgtatctgca ggatagaaag
     1501 ctggcaggca tcctggtgga gctggccgga ataacaggcg atgctgcaca gatcgtcatt
     1561 ggcgccggga ttaacgtggc tatgaggcgc gtggaggaaa gcgtggtcaa tcagggctgg
     1621 atcacactgc aggaagcagg gattaacctg gacaggaata ctctggccgc tacgctgatc
     1681 cgagagctgc gggcagccct ggaactgttc gagcaggaag gcctggctcc atatctgcca
     1741 cggtgggaga agctggataa cttcatcaat agacccgtga agctgatcat tggggacaaa
     1801 gagattttcg ggattagccg ggggattgat aaacagggag ccctgctgct ggaacaggac
     1861 ggagttatca aaccctggat gggcggagaa atcagtctgc ggtctgccga aaagctgcag
     1921 ctgcctcccc tggagcgcct gaccctggac taatagctcg agcatgcatc tagagggccc
     1981 tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag
     2041 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     2101 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     2161 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     2221 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     2281 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     2341 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     2401 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggcatccc
     2461 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     2521 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     2581 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     2641 ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa aaaatgagct
     2701 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     2761 aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca attagtcagc
     2821 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2881 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     2941 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3001 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3061 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     3121 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     3181 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     3241 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     3301 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     3361 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     3421 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     3481 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     3541 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     3601 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     3661 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     3721 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     3781 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     3841 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     3901 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     3961 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4021 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4081 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     4141 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4201 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     4261 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     4321 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     4381 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     4441 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4501 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4561 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4621 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4681 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4741 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4801 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4861 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4921 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4981 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5041 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5101 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     5161 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5221 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5281 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5341 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5401 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5461 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5521 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5581 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5641 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5701 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5761 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5821 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5881 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5941 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6001 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6061 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6121 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6181 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6241 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6301 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6361 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6421 ccacctgacg tc
//
