LOCUS       Exported                6339 bp ds-DNA     circular SYN 09-OCT-2017
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 27, 2017 from SnapGene 4.1.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    863..879
                     /label=T7 promoter primer
     misc_feature    895..902
                     /label=NotI
     CDS             907..909
                     /codon_start=1
                     /label=start
                     /translation="M"
     CDS             910..936
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             940..966
                     /codon_start=1
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             970..996
                     /codon_start=1
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    997..1002
                     /label=NheI
     CDS             1003..1773
                     /codon_start=1
                     /label=BirA (humanized)
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    1006..1008
                     /label=Q65P
     misc_feature    1072..1074
                     /label=I87V
     misc_feature    1156..1158
                     /label=G115G
     misc_feature    1165..1167
                     /label=R118S
     misc_feature    1231..1233
                     /label=E141K
     misc_difference 1234..1236
                     /label=Q142R
     misc_feature    1258..1260
                     /label=S150G
     misc_feature    1261..1263
                     /label=L151P
     misc_feature    1288..1290
                     /label=V160A
     misc_feature    1384..1386
                     /label=T192A
     misc_feature    1390..1392
                     /label=K194I
     misc_feature    1435..1437
                     /label=M209V
     misc_structure  1681..1683
                     /label=G291G
     misc_feature    1723..1725
                     /label=I305V
     misc_feature    1774..1779
                     /label=EcoRI
     misc_feature    1780..1860
                     /label=NLS
     misc_feature    1861..1863
                     /label=Stop
     misc_feature    1864..1869
                     /label=XhoI
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gccaccatgt acccgtatga tgttccggat tacgctggct atccctacga cgtgcccgac
      961 tatgccgggt acccctatga cgtcccagac tacgcagcta gcatcccgct gctgaacgct
     1021 aaacagattc tgggacagct ggacggcggg agcgtggcag tcctgcctgt ggtcgactcc
     1081 accaatcagt acctgctgga tcgaatcggc gagctgaaga gtggggatgc ttgcattgca
     1141 gaatatcagc aggcagggag aggaagcaga gggaggaaat ggttctctcc ttttggagct
     1201 aacctgtacc tgagtatgtt ttggcgcctg aagcggggac cagcagcaat cggcctgggc
     1261 ccggtcatcg gaattgtcat ggcagaagcg ctgcgaaagc tgggagcaga caaggtgcga
     1321 gtcaaatggc ccaatgacct gtatctgcag gatagaaagc tggcaggcat cctggtggag
     1381 ctggccggaa taacaggcga tgctgcacag atcgtcattg gcgccgggat taacgtggct
     1441 atgaggcgcg tggaggaaag cgtggtcaat cagggctgga tcacactgca ggaagcaggg
     1501 attaacctgg acaggaatac tctggccgct atgctgatcc gagagctgcg ggcagccctg
     1561 gaactgttcg agcaggaagg cctggctcca tatctgtcac ggtgggagaa gctggataac
     1621 ttcatcaata gacccgtgaa gctgatcatt ggggacaaag agattttcgg gattagccgg
     1681 gggattgata aacagggagc cctgctgctg gaacaggacg gagttatcaa accctggatg
     1741 ggcggagaaa tcagtctgcg gtctgccgaa aaggaattca gcagggccga ccccaagaag
     1801 aagaggaagg tggaccccaa gaagaagagg aaggtggacc ccaagaagaa gaggaaggtg
     1861 tgactcgagc atgcatctag agggccctat tctatagtgt cacctaaatg ctagagctcg
     1921 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     1981 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     2041 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     2101 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     2161 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     2221 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     2281 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     2341 ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct
     2401 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     2461 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     2521 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat
     2581 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     2641 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat
     2701 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     2761 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     2821 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     2881 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     2941 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     3001 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     3061 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     3121 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     3181 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     3241 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     3301 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     3361 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     3421 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     3481 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     3541 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     3601 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     3661 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     3721 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     3781 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     3841 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     3901 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     3961 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     4021 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4081 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     4141 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     4201 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4261 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4321 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     4381 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4441 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4501 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4561 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4621 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4681 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4741 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     4801 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4861 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4921 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4981 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     5041 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5101 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5161 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5221 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5281 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5341 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5401 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5461 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5521 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5581 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5641 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5701 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5761 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5821 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5881 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5941 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6001 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6061 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6121 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6181 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6241 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6301 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
