LOCUS Exported 8669 bp ds-DNA circular SYN 24-SEP-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8669) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 12, 2018 from SnapGene 4.1.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8669 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 14..566 /label=CMV_immearly_promoter misc_feature 523..543 /label=CMVf misc_feature 523..543 /label=CMVf(1) misc_feature 595..602 /label=attR1 misc_feature 595..602 /label=attR1+teminus misc_feature 603..619 /label=attB1 misc_feature 619..624 /label=BstBI misc_feature 622..631 /label=Shine-Dalgarno misc_feature 634..636 /label=kozak CDS 637..639 /codon_start=1 /label=Start /label=Mito2 /translation="M" CDS 640..717 /label=C1(1-29)P450 CDS 718..729 /label=GS linker(1) CDS 731..738 /label=NotI CDS 739..795 /label=GS linker CDS 796..1566 /codon_start=1 /label=BirA (humanized) /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK" misc_feature 799..801 /label=Q65P misc_feature 865..867 /label=I87V misc_feature 949..951 /label=G115G misc_feature 958..960 /label=R118S misc_feature 1024..1026 /label=E141K misc_difference 1027..1029 /label=Q142R misc_feature 1051..1053 /label=S150G misc_feature 1054..1056 /label=L151P misc_feature 1081..1083 /label=V160A misc_feature 1177..1179 /label=T192A misc_difference 1183..1185 /label=K194I misc_feature 1228..1230 /label=M209V misc_structure 1474..1476 /label=G291G misc_feature 1516..1518 /label=I305V misc_feature 1567..1572 /label=NheI misc_feature 1573..1614 /label=V5 tag misc_feature 1615..1617 /label=STOP misc_feature complement(1619..1628) /label=attB2 misc_feature 1629..1643 /label=attR2 misc_feature 1644..1649 /label=NheI misc_feature 2341..2365 /label=Sequencing primer_fwd_3059 misc_feature 2389..2569 /label=HIV1 3' LTR primer_bind complement(2963..2983) /label=T7 primer_bind complement(2992..3009) /label=M13-fwd CDS 3080..3148 /label=LacZ alpha rep_origin 3167..3473 /label=F1 ori CDS 3934..4593 /label=AmpR rep_origin 4691..5373 /label=ColE1 origin misc_binding 5713..5735 /label=LacO primer_bind 5741..5761 /label=M13-rev primer_bind 5779..5798 /label=T3 misc_feature 6002..6027 /label=Fwd sequencing primer_6720 misc_feature 6051..6231 /label=HIV1 5'LTR misc_feature 6342..6386 /label=Psi Packaging Signal misc_feature 6896..7129 /label=RRE misc_feature 7153..7175 /label=Sequencing primer_Rev_7893 ORIGIN 1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt 601 ttgtacaaaa aagcaggctt cgaaggagat agaaccatgg accctgtggt cgtgctgggg 661 ctgtgcctga gctgtctgct gctgctgagc ctgtggaagc agtcctacgg aggcggggga 721 tcaggaagcg gcggccgcgg atcaggaagc ggatcaggaa gcggatcagg aagcggatca 781 ggaagcggat caggaatccc gctgctgaac gctaaacaga ttctgggaca gctggacggc 841 gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc agtacctgct ggatcgaatc 901 ggcgagctga agagtgggga tgcttgcatt gcagaatatc agcaggcagg gagaggaagc 961 agagggagga aatggttctc tccttttgga gctaacctgt acctgagtat gttttggcgc 1021 ctgaagcggg gaccagcagc aatcggcctg ggcccggtca tcggaattgt catggcagaa 1081 gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat ggcccaatga cctgtatctg 1141 caggatagaa agctggcagg catcctggtg gagctggccg gaataacagg cgatgctgca 1201 cagatcgtca ttggcgccgg gattaacgtg gctatgaggc gcgtggagga aagcgtggtc 1261 aatcagggct ggatcacact gcaggaagca gggattaacc tggacaggaa tactctggcc 1321 gctatgctga tccgagagct gcgggcagcc ctggaactgt tcgagcagga aggcctggct 1381 ccatatctgt cacggtggga gaagctggat aacttcatca atagacccgt gaagctgatc 1441 attggggaca aagagatttt cgggattagc cgggggattg ataaacaggg agccctgctg 1501 ctggaacagg acggagttat caaaccctgg atgggcggag aaatcagtct gcggtctgcc 1561 gaaaaggcta gcggcaagcc catccccaac cccctgctgg gcctggacag cacctaagac 1621 ccagctttct tgtacaaagt ggtgctagcg ctaaccggtg gcgcgttaag tcgacaatca 1681 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 1741 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 1801 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 1861 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 1921 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 1981 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 2041 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg 2101 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 2161 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 2221 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca 2281 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 2341 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg 2401 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 2461 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 2521 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt 2581 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 2641 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 2701 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 2761 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa 2821 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 2881 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 2941 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt 3001 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 3061 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 3121 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc 3181 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 3241 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 3301 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 3361 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 3421 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 3481 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 3541 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac 3601 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 3661 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 3721 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 3781 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 3841 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 3901 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 3961 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 4021 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 4081 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 4141 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 4201 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 4261 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 4321 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 4381 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 4441 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 4501 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 4561 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 4621 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 4681 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 4741 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 4801 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 4861 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 4921 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 4981 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 5041 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 5101 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 5161 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 5221 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 5281 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 5341 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 5401 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 5461 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 5521 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 5581 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 5641 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 5701 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat 5761 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct 5821 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac 5881 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg 5941 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat 6001 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct 6061 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 6121 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 6181 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc 6241 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct 6301 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 6361 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 6421 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 6481 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 6541 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 6601 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 6661 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 6721 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg 6781 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 6841 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 6901 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc 6961 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 7021 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 7081 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 7141 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 7201 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 7261 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 7321 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 7381 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 7441 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 7501 ttcagaccca cctcccaacc ccgaggggac ccttgcgcct tttccaaggc agccctgggt 7561 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg 7621 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg 7681 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg 7741 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac 7801 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct 7861 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg 7921 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc 7981 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggtac 8041 caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac 8101 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga 8161 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact 8221 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat 8281 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga 8341 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg 8401 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ctgcagctgc agtagtaaga 8461 attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 8521 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 8581 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 8641 agatccactt tggcgccggc tcgaggggg //