LOCUS       Exported                7393 bp ds-DNA     circular SYN 08-MAY-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 8, 2018 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        236..852
                     /label=CMV_immearly_promoter
     misc_feature    315..602
                     /label=CAG_enhancer
     misc_feature    769..789
                     /label=CMV_fwd_primer
     promoter        863..881
                     /label=T7_promoter
     misc_feature    938..2164
                     /label=bArr2
     misc_feature    2084..2086
                     /label=383
     misc_feature    2111..2113
                     /label=392
     misc_feature    2114..2116
                     /label=393
     misc_feature    2171..2899
                     /label=TEV-NIaP
     misc_feature    complement(2970..2987)
                     /label=BGH_rev_primer
     terminator      2973..3200
                     /label=bGH_PA_terminator
     rep_origin      3263..3569
                     /label=f1_origin
     misc_feature    complement(3683..3703)
                     /label=pBABE_3_primer
     misc_feature    complement(3689..3904)
                     /label=SV40_enhancer
     promoter        3701..3969
                     /label=SV40_promoter
     rep_origin      3868..3945
                     /label=SV40_origin
     misc_feature    3930..3949
                     /label=SV40pro_F_primer
     CDS             4101..4895
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      5072..5191
                     /label=SV40_PA_terminator
     misc_feature    5160..5179
                     /label=EBV_rev_primer
     promoter        complement(5235..5253)
                     /label=M13_reverse_primer
     misc_feature    complement(5252..5274)
                     /label=M13_pUC_rev_primer
     promoter        complement(5288..5317)
                     /label=lac_promoter
     gene            complement(6397..7257)
                     /label=Ampicillin
     promoter        complement(7299..7327)
                     /label=AmpR_promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccaccatg ggggagaaac ccgggaccag
      961 ggtcttcaag aagtcgagcc ctaactgcaa gctcaccgtg tacttgggca agcgggactt
     1021 cgtagatcac ctggacaaag tggaccctgt agatggcgtg gtgcttgtgg accctgacta
     1081 cctgaaggac cgcaaagtgt ttgtgaccct cacctgcgcc ttccgctatg gccgtgaaga
     1141 cctggatgtg ctgggcttgt ccttccgcaa agacctgttc atcgccacct accaggcctt
     1201 ccccccggtg cccaacccac cccggccccc cacccgcctg caggaccggc tgctgaggaa
     1261 gctgggccag catgcccacc ccttcttctt caccataccc cagaatcttc catgctccgt
     1321 cacactgcag ccaggcccag aggatacagg aaaggcctgc ggcgtagact ttgagattcg
     1381 agccttctgt gctaaatcac tagaagagaa aagccacaaa aggaactctg tgcggctggt
     1441 gatccgaaag gtgcagttcg ccccggagaa acccggcccc cagccttcag ccgaaaccac
     1501 acgccacttc ctcatgtctg accggtccct gcacctcgag gcttccctgg acaaggagct
     1561 gtactaccat ggggagcccc tcaatgtaaa tgtccacgtc accaacaact ccaccaagac
     1621 cgtcaagaag atcaaagtct ctgtgagaca gtacgccgac atctgcctct tcagcaccgc
     1681 ccagtacaag tgtcctgtgg ctcaactcga acaagatgac caggtatctc ccagctccac
     1741 attctgtaag gtgtacacca taaccccact gctcagcgac aaccgggaga agcggggtct
     1801 cgccctggat gggaaactca agcacgagga caccaacctg gcttccagca ccatcgtgaa
     1861 ggagggtgcc aacaaggagg tgctgggaat cctggtgtcc tacagggtca aggtgaagct
     1921 ggtggtgtct cgaggcgggg atgtctctgt ggagctgcct tttgttctta tgcaccccaa
     1981 gccccacgac cacatccccc tccccagacc ccagtcagcc gctccggaga cagatgtccc
     2041 tgtggacacc aacctcattg aatttgatac caactatgcc acagatgatg acattgtgtt
     2101 tgaggacttt gcccggcttc ggctgaaggg gatgaaggat gacgactatg atgatcaact
     2161 ctgcggatcc ggtgagagtc tcttcaaagg gccgcgcgat tataacccca tctcatccac
     2221 aatttgtcac ctcacaaatg agtccgatgg acatacaaca agcctttacg gcattggctt
     2281 cggtccattc atcatcacca acaagcatct tttcagacgc aataacggca ccttgctggt
     2341 gcagtccctc cacggcgtct ttaaagtcaa gaacaccacg acccttcagc aacacctgat
     2401 cgacggaagg gatatgatta tcattagaat gcccaaagat ttcccgccat ttcctcagaa
     2461 actcaagttt agagagccac agagggaaga aaggatctgc ttggtgacaa caaattttca
     2521 aactaagtct atgtcatcca tggtatcaga taccagctgt acgtttccta gttcagacgg
     2581 aatattctgg aagcattgga tacagacaaa ggacggccaa tgcggtagtc cactggtgtc
     2641 tactcgggat ggtttcatcg tcggcattca tagcgccagc aacttcacca atactaataa
     2701 ctacttcacc agcgtgccca agaatttcat ggagcttctg acaaaccagg aagcccagca
     2761 gtgggtttct ggatggcgct tgaacgctga ctccgttctg tggggaggcc ataaagtgtt
     2821 tatgagtaaa cctgaagaac cctttcagcc ggtgaaagag gccactcagc ttatgaatga
     2881 gctggtttat agccagtagg aattctgcag atatccagca cagtggcggc cgctcgagtc
     2941 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     3001 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     3061 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     3121 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     3181 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     3241 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3301 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3361 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3421 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     3481 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     3541 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     3601 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     3661 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     3721 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     3781 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3841 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3901 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     3961 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     4021 ctcccgggag cttgtatatc cattttcgga tctgatcaat tttcggatct gatcaagaga
     4081 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     4141 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     4201 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     4261 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     4321 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     4381 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     4441 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     4501 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     4561 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     4621 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     4681 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     4741 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     4801 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     4861 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     4921 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     4981 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     5041 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     5101 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5161 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     5221 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5281 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5341 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     5401 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5461 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5521 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5581 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5641 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5701 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5761 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5821 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5881 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5941 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6001 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6061 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6121 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt
     6181 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6241 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6301 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6361 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     6421 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6481 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6541 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6601 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6661 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6721 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6781 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6841 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6901 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6961 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7021 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7081 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7141 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7201 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7261 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     7321 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     7381 gccacctgac gtc
//
