LOCUS       Exported                8454 bp ds-DNA     circular SYN 08-MAY-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 8, 2018 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
     misc_feature    156..4556
                     /label=from p1
     misc_feature    156..640
                     /label=Human Synapsin promoter
     misc_feature    671..704
                     /label=lox2722
     misc_feature    755..788
                     /label=loxP
     misc_feature    complement(803..1519)
                     /label=mCherry
     misc_feature    complement(1556..2449)
                     /label=ABIcs
     misc_feature    complement(2450..2485)
                     /label=linker
     misc_feature    complement(2486..2566)
                     /label=V2 tail
     misc_feature    complement(2567..4342)
                     /label=hM3Dq
     misc_feature    complement(4343..4414)
                     /label=Flag
     misc_feature    complement(4439..4472)
                     /label=lox2722
     misc_feature    complement(4523..4556)
                     /label=loxP
     misc_feature    4571..5179
                     /label=WPRE
     polyA_signal    5199..5677
                     /label=hGH polyA
     misc_feature    5717..5857
                     /label=R-ITR
     rep_origin      5949..6255
                     /label=f1 Origin
     misc_feature    6774..7631
                     /label=AmpR
     rep_origin      7782..8449
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat cctctagagt
      661 cgactccgga ataacttcgt ataggatact ttatacgaag ttatgcagaa tggtagctgg
      721 attgtagctg ctattagcaa tatgaaacct cttaataact tcgtatagca tacattatac
      781 gaagttatgg cgcgccgaat tcctaaccgg tcttgtagag ctcatccata cctccggtgg
      841 aatggcggcc ttcggctctc tcatattgtt ccacgatagt gtagtcctca ttgtgagagg
      901 tgatgtccag cttgatattg acattataag cgccagggag ctgcacaggt ttcttggcct
      961 tgtatgtggt tttgacctct gcgtcgtagt gacctccgtc tttcaatttc agtctctgtt
     1021 tgatttctcc tttcagagcg ccatcctcgg gatacattcg ttctgaagat gcctcccagc
     1081 ccatagtctt tttctgcatt acgggtccgt cagaggggaa gttagtgccg cgcaatttca
     1141 ctttatagat aaactcaccg tcctgcaggg aagaatcctg ggtcaccgtc accacccctc
     1201 catcctcgaa attcatcacc cgctcccact taaaaccttc ggggaaagac agtttcaggt
     1261 agtcaggtat atctgctggg tgtttgacat aggccttgct tccgtacata aattgggggg
     1321 agagaatgtc ccaggcaaat gggagaggac cccccttggt gactttgagt ttagcagttt
     1381 gagtcccttc atacggtcga ccttcccctt cgccctctat ttcgaactcg tggccattga
     1441 cgctaccctc catatgaact ttgaaccgca taaactcctt tattatggcc atgttgtcct
     1501 cctctccctt ggacaccata ccggtggacc cgcccccaga gccgccacct gtacacttca
     1561 gatccaccac gaccacagag atgttatcct tagacccccg ctgaatggcg agctttgaca
     1621 ggtattctgc ggcactcatg gccgcggggt ccttgccttc cttccgtctt tcatcggcca
     1681 gcagagatgc gtcgcctgcc acagcgtttt ttttatgcca gagcagaatc cgttttcttg
     1741 ccatttcaca ggcctcctcg tcagtcatca cgtcccaaac tccgtcagaa gccagaatca
     1801 gacagtcgtc ttccttaacg cgtttcacgg cagtgacttc agggtcgggg atgatggagg
     1861 gtttcagata ccgatcgccg atggacctac tcatagcaag aaccccgaat acgcgggctc
     1921 cattccactg gatcacctta ccacctgcgg cctcgatcct ggcggcctcg tcttctcgat
     1981 cgggcttgtg atccacggac aagggcaggg cagttttccc tcgacacagc actgccctgg
     2041 agtctccaca gttggccaca aagatatgtg aagggaacac gactgccact acagaagtgc
     2101 tccccacggt ctcgggagcc acgctttcaa tctctgagtc cactcgcaaa aaactgttga
     2161 agagcgcttt tttccacttt tccagccagg tgtctccgtc acagagcatc ggcttttctt
     2221 tagctatttc ctctgcgaga gcgagatgca tgcgctccct gcagtaatta gccacctgag
     2281 atccgccatg cccgtcataa acgccgaaaa agtgagcagc tgactgagga tcaaatcggc
     2341 cgtcaagcat agacccggag ctggactgaa ggaatcgtgg aatggtagaa acggcagcct
     2401 ccatttccgg tcttctcccg cagatggacg tgaatccata gaggggcact gtacagcttc
     2461 cccccccact tccccctcca tcgatcgatg aagtgtcctt ggccagggag gagctggcgg
     2521 tggtgcagga ctcatcttgg ggacccaggt cgggtgggtc gcgtcccagg gcttgttcgg
     2581 gggctcgctt gtggaaaatc acggactgtc tctgctggta ctgctgcttc cgtctctttt
     2641 tcttgtcgca ttggcacaaa aggagcatct tgaatgtcgt cctgaaggtt ttgttgcaca
     2701 aggcgtaaca aacaggattc acggtagagt tgatgtaaca cagccagtag ccaagattcc
     2761 agaaggtttt aggaatacag gaatcacaaa atgtgttcac gagcaccatg atgttgtaag
     2821 gggtccatgt tatgatgaat gccaacagga ttgcactaag tgtttgggcg gctttttttt
     2881 ccttgaccag gctcatcctt tttctcttag tgatctggga ccgggtcttc agcgcgaacc
     2941 tctttgcgag agttgcttcc ttaaaggaca agggcagggt agcggtagat ttgccgacgc
     3001 tgctgttcac gtcactagtt tttgcagtgt ctacagcaga ctcgagttga attggcagct
     3061 tagagaaaga cttggggaaa ctacctccat catcaacgga tttctgggcc tgcagtttat
     3121 ctgctttgcg ctcgaggtcg accatgccca gttcctcctc gggcacctgg aggttatcgc
     3181 tgctgggaag cttagttgag ttcaggatag tggaatgtcc tggcaatttc agcacaatac
     3241 tgtagatagc gcgggtttca ctgccgatat cctcttcgtc agagcttgcg gagttttcca
     3301 gggaagcggc agcgtcgtta ttattccagg agtcactact tgagtgatcc tggtccatct
     3361 gttctgagct gggtttccaa gacttggtgg tgaaccaaaa atgacacctt ccatatttcc
     3421 gccggttgga gcgcttcatg gactgctgtt ggagctcgta tgatgagcaa gatcgagaag
     3481 agccggtggg atgcacaaag ttctctgttt ccgcctccgt cccgcttgct tgaaggccgg
     3541 ccagctcttt agtccgcttc tcagtctctt tgtagatgcg ccaataaagg atggtcatga
     3601 tggtgacagg catgtaaaag cccgcgattg cagtaccgaa ggtgatagta ggttcggaca
     3661 agaactgtat aaaacactcg ccggggggca cggtccgctt cccaacgaaa tactgccaga
     3721 acaggatagc tggggcccag agtacgaagg aaatcaccca ggccaggcca atcataactc
     3781 cagcccgttt ggttgtcctc ttagctcgat aggtcaaagg cctggtaatg gagaaatagc
     3841 gatcaaaaga tatgacgagg agattcatga ctgaagcgtt tgaggccaca cagtctatgg
     3901 ccagccagag gtcgcacgcg aggtttccca gtgcccagcg gttcataatg atgtaggtgg
     3961 taaagagatt catagagatc actccgatga tcaagtcagc gcacgccagg ctcagcagga
     4021 agtaattatt cacggtttta agctgcttat tgactttaaa ggagacaatc accaggatgt
     4081 tccctattat cgttacgaga gccaaaatgc cagtgaggaa agcaatgaaa accacctgcc
     4141 aaacggtgtg ccctcccagt gggtcatcag tggtgccgtc gggggaggaa aaatttccag
     4201 ctgcccttga aacattatag gagccgaagt gagtcacggt gcctggaggc aggcctgcgt
     4261 cggatgggct atgaatccaa gagctggaga tattgggaaa gagtggactg gtcgtagaat
     4321 tattgtgaag agtcatatcg atcttgtcat catcgtcttt atagtcggcg aaaaccaagc
     4381 agaaaatata ggacagagcg atgatagttt tcatggtggc tctagaggat ccgctagcat
     4441 aacttcgtat aaagtatcct atacgaagtt atttgcctta acccagaaat tatcactgtt
     4501 attctttaga atggtgcaaa gaataacttc gtataatgta tgctatacga agttatgaat
     4561 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     4621 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4681 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4741 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4801 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4861 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4921 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4981 cgtcctttcc ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct
     5041 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5101 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5161 cctccccgca tcgataccga gcgctgctcg agagatctac gggtggcatc cctgtgaccc
     5221 ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct
     5281 aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg
     5341 gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt
     5401 ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc
     5461 ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat
     5521 gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag
     5581 gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg
     5641 attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg
     5701 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     5761 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     5821 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     5881 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     5941 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6001 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6061 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6121 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     6181 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6241 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     6301 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6361 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     6421 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     6481 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     6541 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     6601 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     6661 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6721 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6781 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6841 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6901 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6961 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7021 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7081 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7141 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7201 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7261 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7321 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7381 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7441 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7501 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7561 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7621 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7681 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7741 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7801 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7861 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7921 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     7981 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8041 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8101 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8161 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8221 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8281 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8341 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8401 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
